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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

28卷

期数:

2022年04期

页码:

343-347

栏目:

外泌体专题

出版日期:

2022-07-20

文章信息/Info

Title:

The effect of M2 macrophage-derived exosomes on skin wound healing

文章编号:

1006-8147（2022）04-0343-05

作者:

王敏¹; 邢逸¹; 徐海楠¹; 王子祎¹; 孙逊²; 赵艳梅³; 赵艳红¹

（1.天津医科大学口腔医院正畸科，天津 300070；2.天津大学天津医院脊柱外科，天津300211；3.天津大学应急医学研究院，天津300072）

Author(s):

WANG Min¹; XING Yi¹; XU Hai-nan¹; WANG Zi-yi¹; SUN Xun²; ZHAO Yan-mei³; ZHAO Yan-hong¹

（1.Department of Orthodontics，Stomatology Hospital，Tianjin Medical University，Tianjin 300070，China; 2.Department of Spinal Surgery，Tianjin Hospital，Tianjin University，Tianjin 300211，China; 3.Academy of Emergency Medicine，Tianjin University，Tianjin 300072，China）

关键词:

M2型巨噬细胞; 外泌体; L929真皮成纤维细胞; 创面愈合

Keywords:

M2 macrophage;  exosomes;  L929 dermal fibroblasts;  wound healing

分类号:

R782.4

DOI:

-

文献标志码:

A

摘要:

目的：探究M2型巨噬细胞来源的外泌体（M2exos）对成纤维细胞增殖、迁移的影响。方法：极化RAW264.7细胞并收集M2型巨噬细胞上清，提取并鉴定M2exos。通过免疫荧光观察L929真皮成纤维细胞对M2exos的摄取情况，设对照组和M2exos组，通过CCK-8、细胞划痕实验分别观察在0、24、48、72 h对成纤维细胞的增殖和迁移作用。建立皮肤创口模型，随机将12只C57BL/6J小鼠分为PBS组和M2exos组。两组分别注射200 μL PBS、M2exos（500 μg/mL），在第1、4、7天观察、拍摄记录，并取材进行HE染色。结果：成功极化得到M2型巨噬细胞，提取和鉴定了M2exos。与对照组相比，M2exos组在48、72 h对成纤维细胞具有促进增殖（t=26.73，P＜0.000 1；t=6.648，P＜0.01）、迁移（t=5.196，P＜0.05；t=22.00，P＜0.000 1）作用。对C57BL/6J小鼠的研究显示，与PBS组相比，在第4、7天时M2exos组创面显著缩小（t=32.80、 51.16，均P＜0.000 1）。结论：M2exos通过促进成纤维细胞增殖和迁移，对小鼠创面愈合有显著的促进作用。

Abstract:

Objective：To investigate the effect of M2 macrophage-derived exosomes（M2exos）on the proliferation and migration of fibroblasts. Methods：RAW264.7 cells were polarized and M2 macrophages supernatant was collected to extract and identify M2exos. The uptake of M2exos by L929 dermal fibroblasts was observed by immunofluorescence，and control group and M2exos group were set up，the proliferation and migration of fibroblasts were observed by CCK-8 assay and cell scratch assay at 0，24，48 and 72 h .The skin wound model was established. Twelve C57BL/6J mice were randomly divided into PBS group and M2exos group. The two groups were injected with 200 μL PBS and M2exos（500 μg/mL），respectively. The mice were observed and recorded on the 1st，4th and 7th day，and samples were taken for HE staining. Results：M2 macrophages were successfully polarized，extracted and identified M2exos. CCK-8 assay showed that M2exos promoted the proliferation （t=26.73，P＜0.000 1；t=6.648，P＜0.01）and migration（t =5.196，P＜0.05；t=22.00，P＜0.000 1）of fibroblasts at 48 h and 72 h compared with PBS group. The study of C57BL / 6J mice showed that，the wound surface of M2exos group was significantly smaller than those in PBS group on the 4th and 7th day（t=32.80，51.16，all P＜0.000 1）. Conclusion：M2exos can promote wound healing in mice by promoting the proliferation and migration of fibroblasts.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金（81800931）；天津市科技计划项目（20JCYBJC01440）；天津自然科学基金（18JCJQJC47900）
作者简介:王敏（1996-），女，硕士在读，研究方向：口腔正畸学及口腔相关的骨、软骨组织再生；通信作者：赵艳红，E-mail：leafzh@126.com。

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更新日期/Last Update: 2022-07-20