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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

26卷

期数:

2020年02期

页码:

103-107

栏目:

基础医学

出版日期:

2020-04-30

文章信息/Info

Title:

TNF-α-induced circMAN1A2 promotes migration, invasion and proliferation of cervical cancer cells

文章编号:

1006-8147(2020)02-0103-05

作者:

白丽丽; 汤华

（天津医科大学基础医学院病原生物学系，天津市生命科学中心实验室，天津市炎症生物学重点实验室，天津300070）

Author(s):

BAI Li-li; TANG Hua

（Department of Pathogenic Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin Life Science Research Center, Tianjin Key Laboratory of Inflammation Biology, Tianjin 300070, China）

关键词:

circMAN1A2; 宫颈癌; 迁移; 侵袭; 增殖

Keywords:

circMAN1A2;  cervical cancer;  migration;  invasion;  proliferation

分类号:

R737.33

DOI:

-

文献标志码:

A

摘要:

目的：探讨肿瘤坏死因子（TNF）-α诱导的circMAN1A2对宫颈癌细胞恶性行为的影响。方法：RNA高通量测序检测TNF-α处理后人宫颈癌HeLa细胞中circRNA的表达差异性。RT-qPCR方法验证TNF-α对circMAN1A2的影响。RNase R耐受性实验验证circMAN1A2是否耐受RNase R消化。核质RNA分离实验研究circMAN1A2在HeLa细胞的定位；Transwell迁移侵袭实验、MTT实验、集落形成实验研究circMAN1A2对宫颈癌细胞恶性行为的影响。结果：RNA高通量测序检测到98种表达上调和63种表达下调的circRNA。TNF-α处理后circMAN1A2表达上调，其能够耐受RNase R消化，主要定位于HeLa细胞胞核中。细胞功能实验表明circMAN1A2能够促进宫颈癌细胞的迁移、侵袭和增殖。结论：TNF-α诱导的circMAN1A2促进宫颈癌细胞的迁移、侵袭和增殖。

Abstract:

Objective: To identify the effect of tumor necrosis factor(TNF)-α-induced circMAN1A2 on the malignant behavior of cervical cancer cells. Methods: RNA high-throughput sequencing was used to detect the difference of circRNA expression in human cervical cancer HeLa cells treated with TNF-α. RT-qPCR was used to verify the effect of TNF-α on circMAN1A2. The RNase R resistance assay verified whether circMAN1A2 was resistant to RNase R digestion. RNA isolation experiment was performed to investigate the localization of circMAN1A2 in HeLa cells. Transwell migration/invasion assay, MTT assay, and colony formation assay were used to study the effects of circMAN1A2 on the malignant behavior of cervical cancer cells. Results: RNA high-throughput sequencing found 98 up-regulated and 63 down-regulated circRNA. circMAN1A2 was up-regulated after TNF-α treatment, which was resistant to RNase R digestion and was mainly localized in the nuclear of HeLa cells. Cell function experiments showed that circMAN1A2 could promote the migration, invasion and proliferation of cervical cancer cells. Conclusion: TNF-α-induced circMAN1A2 promotes the migration, invasion and proliferation of cervical cancer cells.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金重大研究计划集成项目（91629302）
作者简介 白丽丽（1988-），女，硕士在读，研究方向：病原生物学；
通信作者: 汤华，E-mail:htang2002@yahoo.com。

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更新日期/Last Update: 2020-06-02