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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

26卷

期数:

2020年01期

页码:

8-12

栏目:

基础医学

出版日期:

2020-04-06

文章信息/Info

Title:

miR-3685 inhibits migration, invasion and growth of cervical cancer cells by targeting CTTN

文章编号:

1006-8147(2020)01-0008-05

作者:

闫晓芳; 谢 虹; 汤 华

（天津医科大学基础医学院病原生物学系，天津市生命科学中心实验室，天津市炎症生物学重点实验室，天津 300070）

Author(s):

YAN Xiao-fang; XIE Hong; TANG Hua

（Department of Pathogenic Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin Life Science Research Center, Tianjin Key Laboratory of Inflammation Biology, Tianjin 300070, China )

关键词:

miR-3685; 宫颈癌; CTTN; 上皮间质转化; 细胞周期

Keywords:

miR-3685;  cervical cancer;  CTTN;  EMT;  cell cycle NA Not available

分类号:

R737.33

DOI:

-

文献标志码:

A

摘要:

目的：微小RNA (microRNA，miRNA)通常通过与其靶基因的3′非翻译区（3′UTR）结合而调控肿瘤细胞的恶性行为，目前miR-3685对肿瘤细胞恶性行为的影响鲜有报道，本文旨在探讨miR-3685对宫颈癌细胞恶性行为及其分子机制的影响。方法：生物信息学软件预测miR-3685的靶基因，用EGFP报告系统验证。实时荧光定量PCR(RT-qPCR)技术检测miR-3685和CTTN(cortactin)在人类宫颈癌细胞中的表达，用transwell迁移/侵袭实验、Western blot实验、集落形成实验、细胞周期进程实验分别检测了miR-3685和CTTN对HeLa细胞和SiHa细胞的迁移、侵袭能力、EMT进程、细胞增殖能力及细胞周期进程的影响。结果：与对照组相比，过表达miR-3685可抑制宫颈癌HeLa和SiHa细胞的迁移、侵袭及生长，封闭miR-3685，得到相反的结果。过表达CTTN可促进宫颈癌HeLa和SiHa细胞的迁移、侵袭及生长，EGFP报告系统验证CTTN是miR-3685的直接靶基因（*P＜0.05，**P＜0.01，***P＜0.001）。结论：miR-3685通过抑制CTTN的表达而抑制宫颈癌HeLa和SiHa细胞的迁移、侵袭及生长。

Abstract:

Objective: MicroRNAs(miRNAs) usually regulate the malignant behavior of tumor cells by binding to the 3′ untranslated region(3′UTR) of their target genes. But he effect of miR-3685 on the malignant behavior of cervical cancer cells has not been reported. This study aims to investigate the effect of miR-3685 on the malignant behavior and molecular mechanism of cervical cancer cells. Methods: The bioinformatics software was used to predict the target gene of miR-3685 and verified it with the EGFP reporter system.Real-time quantitative PCR (RT-qPCR) technique was used to detect the expression of miR-3685 and CTTN (cortactin) in human cervical cancer cells.Transwell migration/invasion assay, Western blot assay, colony formation assay and flow cytometry assay were used to detect migration, invasion, EMT progression, cell proliferation and cell cycle process of miR-3685 and CTTN in HeLa cells and SiHa cells. Results: Compared with the control group, overexpression of miR-3685 inhibited migration, invasion and growth of HeLa cells and SiHa cells, while blocking miR-3685 gave the opposite result. Overexpression of CTTN promoted migration and invasion and growth of cervical cancer HeLa and SiHa cells. The EGFP reporter system verified that CTTN is a direct target gene for miR-3685. Conclusion: miR-3685 inhibits the migration, invasion and growth of HeLa cells and SiHa cells by inhibiting the expression of CTTN.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金重大研究计划集成项目（91629302）
作者简介 闫晓芳（1993-），女，硕士在读，研究方向：病原生物学；通信作者：汤华，E-mail: htang2002@yahoo.com。

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更新日期/Last Update: 2020-04-16