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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

22卷

期数:

2016年04期

页码:

288-290

栏目:

基础医学

出版日期:

2016-07-19

文章信息/Info

Title:

miR-186 inhibits cervical cancer migration by targetting CEA

文章编号:

1006-8147（2016）04-0288-03

作者:

孙 艳; 齐 政; 王德华; 张艳芳

(天津医科大学总医院妇产科, 天津300052)

Author(s):

SUN Yan; QI Zheng; WANG De-hua; ZHANG Yan-fang

(Department of Obstetrics and Gynecology, General Hospital, Tiajin Medical University, Tianjin 300052,China)

关键词:

miR-186; 宫颈癌; 癌胚抗原; 转移

Keywords:

miR-186; cervical cancer; CEA; migration

分类号:

Q7

DOI:

-

文献标志码:

A

摘要:

目的:验证miR-186通过靶定CEA对宫颈癌细胞生物功能的影响。方法:利用表达谱芯片及荧光定量PCR验证miR-186在宫颈癌中的表达水平；通过Transwell及划痕试验阐述miR-186靶定CEA对宫颈癌细胞转移能力的影响；通过双荧光素酶报告基因方法验证miR-186对癌胚抗原(CEA)的靶定关系。结果:转移性宫颈癌组织中miR-186的表达水平较非转移性宫颈癌组织中偏低；在宫颈癌细胞中上调miR-186的表达水平并靶定CEA能够抑制其转移能力；荧光报告载体试验证实CEA为miR-186的靶基因。结论:miR-186抑制宫颈癌的转移，CEA为miR-186的靶基因。

Abstract:

Objective: To analyze the function of miR-186 dysregulation in cervical cancer. Methods: MicroRNA microarray and qRT-PCR were performed to analyze the expression level of miR-186, then transwell and Wound Healing assay were performed to analyze the effect of miR-186 over-expression on cervical cell lines. In addition, luciferase reporter assay was used to locate the target gene of miR-186. Results: The expression of miR-186 in matastasis cervical cancer was lower than that in matastasis cervical cancer tissues. Ectopic expression of miR-186 inhibited cervical cell migration. The result of Luciferase reporter assay showed that CEA was directly regulated by miR-186. Conclusion: MiR-186 has low expression in matastasis cervical cancer and inhibited cervical cancer cell migration by targeting CEA.

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备注/Memo

备注/Memo:

作者简介 孙艳(1981-),女,住院医师,硕士,研究方向:妇科肿瘤;

通信作者:齐政, E-mail:qizheng@eyou.com。