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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

23卷

期数:

2017年02期

页码:

121-123

栏目:

基础医学

出版日期:

2017-03-20

文章信息/Info

Title:

miR-185 inhibits the migration and angiogenesis of HUVECs induced by VEGF by targeting MRTF-A

文章编号:

1006-8147（2017）02-0121-03

作者:

张 蕊; 李玉明; 赵明峰

(天津市第一中心医院血液科实验室，天津 300457)

Author(s):

ZHANG Rui;  LI Yu-ming;  ZHAO Ming-feng

(Laboratory of Hematology , Tianjin First Central Hospital, Tianjin 300457, China)

关键词:

人脐静脉内皮细胞 ; 迁移; 血管形成; 血管内皮生长因子 ; miR-185

Keywords:

HUVECs;  migration;  angiogenesis;  VEGF;  miR-185

分类号:

R730

DOI:

-

文献标志码:

A

摘要:

目的：探究过表达miR-185是否会抑制血管内皮生长因子 （ VEGF）诱导人脐静脉内皮细胞（HUVECs）迁移及血管形成。方法：在HUVECs中过表达miR-185并利用50 ng/mL的VEGF处理,划痕实验和transwell实验检测HUVECs的迁移情况；Matrigel血管形成实验检测其血管形成情况。 结果：划痕实验和transwell实验表明过表达miR-185后,抑制了VEGF引起的HUVECs迁移, 并降低迁移标志基因MYL9（ MYL9）、CYR61（ CYR61）的表达；Matrigel血管形成实验表明过表达miR-185抑制了VEGF引起的HUVECs血管形成。结论：过表达miR-185可以抑制VEGF引起的HUVECs迁移和血管形成。

Abstract:

Objective: To investigate whether the migration and angiogenesis of HUVECs treated by VEGF could be inhibited by overexpression miR-185. Methods: HUVECs were transfected with miR-185 mimics and treated with 50 ng/mL of VEGF. HUVECs migration ability was tested by wound heal assay and transwell assay; Angiogenesis was tested by Matrigel assay. Results: Wound heal assay and transwell assay confirmed that miR-185 mimics inhibited VEGF-induced HUVECs migration and reduced the expression of migration marker genes MYL9 and CYR61. Meanwhile, Matrigel assay showed that miR-185 mimics inhibited VEGF-induced HUVECs angiogenesis. Conclusion: Overexpression miR-185 could inhibit the migration and angiogenesis of HUVECs induced by VEGF.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目? 天津市卫计委科技基金项目重点项目(2015K215)

作者简介? 张蕊（1988—），女，博士, 研究方向：间充质干细胞向内皮细胞分化及血管形成、肿瘤干细胞、肿瘤机制研究;

通信作者：赵明峰， E-mail: mingfengzhao@sina.com.

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更新日期/Last Update: 2017-03-28