|本期目录/Table of Contents|

[1]孙秀梅,张 飞,田 然,等.Nanog表达上调促进乳腺癌细胞MCF-7的增殖和侵袭[J].天津医科大学学报,2014,20(06):421-425.
 SUN Xiu-mei,ZHANG Fei,TIAN Ran,et al.Up-regulation of Nanog promotes cell proliferation and invasion in breast cancer cells MCF-7[J].Journal of Tianjin Medical University,2014,20(06):421-425.
点击复制

Nanog表达上调促进乳腺癌细胞MCF-7的增殖和侵袭(PDF)
分享到:

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20卷
期数:
2014年06期
页码:
421-425
栏目:
基础医学
出版日期:
2014-11-20

文章信息/Info

Title:
Up-regulation of Nanog promotes cell proliferation and invasion in breast cancer cells MCF-7
文章编号:
1006-8147(2014)06-0421-05
作者:
孙秀梅张 飞田 然刘 媛王智勇杨 毅魏熙胤牛瑞芳
(天津医科大学肿瘤医院公共实验室,国家肿瘤临床医学研究中心,天津市“肿瘤防治”重点实验室,乳腺癌防治教育部重点实验室,天津 300060)
Author(s):
SUN Xiu-mei ZHANG Fei TIAN Ran LIU Yuan WANG Zhi-yong YANG Yi WEI Xi-yin NIU Rui-fang
(Department of Public Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin 300060,China)
关键词:
Nanog增殖迁移侵袭MCF-7乳腺癌
Keywords:
NanogproliferationmigrationinvasionMCF-7breast cancer
分类号:
R737.9
DOI:
-
文献标志码:
A
摘要:
目的:观察Nanog高表达对乳腺癌细胞MCF-7生物学特性的影响。方法:构建重组质粒pcDNA3.1(-)-Nanog,利用免疫荧光观察Nanog高表达后,乳腺癌细胞MCF-7中Nanog的定位。利用平皿克隆分离法获得Nanog高表达的乳腺癌细胞系MCF-7。应用克隆形成实验、Transwell迁移和侵袭实验观察Nanog高表达对乳腺癌细胞MCF-7生物学特性的影响。结果:Nanog在MCF-7细胞中定位于核,Nanog高表达后,乳腺癌细胞MCF-7的增殖、迁移和侵袭能力显著增强。结论:Nanog能够促进乳腺癌细胞MCF-7的增殖、迁移和侵袭能力。
Abstract:
Objective: To observe the effects of up-regulation of Nanog on the biological characteristics of breast cancer cells MCF-7. Methods: A recombinant plasmid pcDNA3.1(-)-Nanog was constructed. Using immunofluorescence, Nanog positioning in MCF-7 was observed after transfected with plasmid pcDNA3.1(-)-Nanog. Breast cancer cell line MCF-7 high expression Nanog was obtained by using cloning separation plate. The effects of up-regulation of Nanog on the biological characteristics of MCF-7 breast cancer cells were observed by clonogenic assay, Transwell migration and invasion assay. Results: Nanog was localized in the nucleus in MCF-7 cells. MCF-7 high expression of Nanog had more significant effect on cell proliferation, migration and invasion. Conclusion: Up-regulation of Nanog could promote cell proliferation, migration and invasion of MCF-7

参考文献/References:

[1]Vergne, Y, Matta J, et al. Breast Cancer and DNA repair capacity:association with use of multivitamin and Calcium supplements[J]. Integr Med (Encinitas), 2013, 12(3): 38[2]Cavaleri F, Sch?ler H R. Nanog: a new recruit to the embryonic stem cell orchestra[J]. Cell, 2003, 113(5): 551
[3]Zhang J, Espinoza L A, Kinders R J, et al. NANOG modulates stemness in human colorectal Cancer[J]. Oncogene, 2013, 32(37): 4397
[4]Ezeh U I, Turek P J, Reijo R A, et al. Human embryonic stem cell genes OCT4, NANOG, STELLAR, and GDF3 are expressed in both seminoma and breast carcinoma[J]. Cancer, 2005, 104(10): 2255
[5]Ye F,?Zhou C,?Cheng Q, et al. Stem-cell-abundant proteins Nanog, Nucleostemin and Musashi1 are highly expressed in malignant cervical epithelial cells[J]. BMC Cancer, 2008, 8: 108
[6]Ibrahim E E, Babaei-Jadidi R, Saadeddin A, et al. Embryonic NANOG activity defines colorectal Cancer stem cells and modulates through AP1- and TCF-dependent mechanisms[J]. Stem Cells, 2012, 30(10): 2076
[7]Han Jing HUA, Zhang Fei, Yu Man, et al. RNA interference-mediated silencing of NANOG reduces cell proliferation and induces G0/G1 cell cycle arrest in breast Cancer cells[J]. Cancer Lett, 2012, 321(1): 80
[8]Shan J,?Shen J,?Liu L , et al. Nanog regulates self-renewal of cancer stem cells through the insulin-like growth factor pathway in human hepatocellular carcinoma[J]. Hepatology, 2012, 56(3): 1004
[9]Ishiguro T, Sato A, Ohata H, et al. Differential expression of nanog1 and nanogp8 in colon Cancer cells[J]. Biochem Biophys Res Commun, 2012, 418(2): 199
[10]Chiou S H, Wang M L, Chou Yu TING, et al. Coexpression of Oct4 and nanog enhances malignancy in lung adenocarcinoma by inducing Cancer stem Cell-Like properties and Epithelial-Mesenchymal transdifferentiation[J]. Cancer Res, 2010, 70(24): 10433
[11]Chiou S H, Yu C C, Huang Chi YANG, et al. Positive correlations of Oct-4 and Nanog in oral Cancer stem-like cells and high-grade oral squamous cell carcinoma[J]. Clin Cancer Res, 2008, 14(13): 4085
[12]Nagata T. Prognostic significance of NANOG and KLF4 for breast Cancer[J]. Breast Cancer, 2012, 30(15, S): 96
[13]Lee M, Nam E J, Kim S W, et al. Prognostic impact of the Cancer stem Cell-Related marker NANOG in ovarian serous carcinoma[J]. Int J Gynecol Cancer, 2012, 22(9): 1489
[14]Meng H M, Zheng P, Wang X Y, et al. Over-expression of Nanog predicts tumor progression and poor prognosis in colorectal Cancer[J]. Cancer Biol Ther, 2010, 9(4): 295
[15]Cao J, Li L, Chen C, et al. RNA interference-mediated silencing of NANOG leads to reduced proliferation and self-renewal, cell cycle arrest and apoptosis in T-cell acute lymphoblastic leukemia cells via the p53 signaling pathway[J]. Leuk Res, 2013, 37(9): 1170.
[16]Sun C, Sun L, Jiang K, et al. NANOG promotes liver Cancer cell invasion by inducing epithelial-mesenchymal transition through NODAL/SMAD3 signaling pathway[J]. Int J Biochem Cell Biol, 2013, 45(6): 1099.
[17]Siu M , Wong E , Kong D . S. H, et al. Stem cell transcription factor NANOG controls cell migration and invasion via dysregulation of E-cadherin and FoxJ1 and contributes to adverse clinical outcome in ovarian cancers[J]. Oncogene, 2013, 32(30): 3500
[18]Luo W R, Li S Y, Peng B L, et al. Embryonic stem cells markers SOX2, OCT4 and Nanog expression and their correlations with epithelial-mesenchymal transition in nasopharyngeal carcinoma[J]. PLoS One, 2013, 8(2): e56324
[19]Siu M , Wong E , Kong D . S. H, et al. Stem cell transcription factor NANOG controls cell migration and invasion via dysregulation of E-cadherin and FoxJ1 and contributes to adverse clinical outcome in ovarian cancers[J]. Oncogene, 2013, 32(30): 3500
[20]Jeter C R, Liu B, Liu X, et al. NANOG promotes Cancer stem cell characteristics and prostate Cancer resistance to androgen deprivation[J]. Oncogene, 2011, 30(36): 3833
[21]Bourguignon L , Earle C, Wong G, et al. Stem cell marker (Nanog) and Stat-3 signaling promote MicroRNA-21 expression and chemoresistance in hyaluronan/CD44-activated head and neck squamous cell carcinoma cells[J]. Oncogene, 2012, 31(2): 149
[22]Zhang J, Espinoza L A, Kinders R J, et al. NANOG modulates stemness in human colorectal Cancer[J]. Oncogene, 2013, 32(37): 4397
[23]Zbinden M, Duquet A, Lorente-Trigos A, et al. NANOG regulates glioma stem cells and is essential in vivo acting in a cross-functional network with GLI1 and p53[J]. EMBO J, 2010, 29(15): 2659
[24]Lu X, Mazur S J, Lin T, et al. The pluripotency factor nanog promotes breast Cancer tumorigenesis and metastasis[J]. Oncogene, 2014, 33(20): 2655

相似文献/References:

[1]马淑晶,张晓燕,徐亚洁,等.青蒿琥酯通过上调神经酰胺抑制肝星状细胞增殖并诱导其凋亡[J].天津医科大学学报,2014,20(04):253.
 MA Shu-jing,ZHANG Xiao-yan,XU Ya-jie,et al.Artesunate inhibits the proliferation of hepatic stellate cells and induces apoptosis by upregulating ceramide[J].Journal of Tianjin Medical University,2014,20(06):253.
[2]王 勇,陈雅婧 综述,岳 丹审校.YB-1对肿瘤增殖调控的研究进展[J].天津医科大学学报,2014,20(04):332.
[3]伦淑敏.肌细胞增强因子2A基因真核表达质粒的构建及对乳腺癌细胞MCF-7增殖能力的影响[J].天津医科大学学报,2014,20(06):429.
 LUN Shu-min.Construction of myocyte enhancer factor 2A eukaryotic expression plasmid and effects on cell proliferation in breast cancer cell line MCF7[J].Journal of Tianjin Medical University,2014,20(06):429.
[4]孙秀梅,张 飞 综 述,牛瑞芳 审校. Nanog及其假基因NanogP8在肿瘤中的研究进展[J].天津医科大学学报,2015,21(03):90.
[5]任宗娜.沉默Notch4基因对乳腺癌细胞系MDA-MB-231增殖和迁移侵袭能力的影响[J].天津医科大学学报,2015,21(06):469.
 REN Zong-na.Inhibition effect of?silencing?? Notch4 gene on the proliferation and migration and invasion activity of? breast cancer cell line?MDA-MB-231[J].Journal of Tianjin Medical University,2015,21(06):469.
[6]姚 芳,张 鹏,王元国.5-杂氮-2′-脱氧胞苷上调CK13表达并抑制人肺鳞癌YTMLC-9细胞增殖[J].天津医科大学学报,2015,21(06):474.
 YAO Fang,ZHANG Peng,WANG Yuan-guo. 5-Aza-2 ′-deoxycytidine inhibits human lung squamous carcinoma YTMLC-9 cell by epigenetically silenced CK13 gene in vitro [J].Journal of Tianjin Medical University,2015,21(06):474.
[7]周岩,宋伟杰,张飞,等.人附睾蛋白4在乳腺癌发生发展中的机制研究[J].天津医科大学学报,2015,21(06):466.
 ZHOU Yan,SONG Wei-jie,ZHANG Fei,et al.Mechanism of human epididymis protein 4 in development and progression of breast cancer[J].Journal of Tianjin Medical University,2015,21(06):466.
[8]黄环静综述,冯玉梅审校.NF-κB信号通路促进乳腺癌细胞增殖和转移机制的研究进展[J].天津医科大学学报,2016,22(03):270.
[9]黄环静.慢病毒载体PLKO.1-shp65的构建及在乳腺癌细胞MDA-MB-231中功能的研究[J].天津医科大学学报,2016,22(05):386.
 HUANG Huan-jing.Construction of p65 shRNA knockdown vector and its effect on breast cancer cell MDA-MB-231[J].Journal of Tianjin Medical University,2016,22(06):386.
[10]文明明,马跃美,赵秀兰,等.炎症因子IL-23 对黑色素瘤细胞增殖侵袭及双向分化的影响[J].天津医科大学学报,2017,23(04):295.
 WEN Ming-ming,MA Yue-mei,ZHAO Xiu-lan,et al.Effects of IL-23 on the herin proliferation,invasion and bi-differentiation? of melanoma cells[J].Journal of Tianjin Medical University,2017,23(06):295.

备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目(81372844: 81071731)

作者简介 孙秀梅(1985-),女,硕士在读,研究方向:生物化学与分子生物学;通信作者:牛瑞芳,E-mail:niuruifang@tjmuch.com。



 

更新日期/Last Update: 2014-12-03