|本期目录/Table of Contents|

[1]文明明,马跃美,赵秀兰,等.炎症因子IL-23 对黑色素瘤细胞增殖侵袭及双向分化的影响[J].天津医科大学学报,2017,23(04):295-299.
 WEN Ming-ming,MA Yue-mei,ZHAO Xiu-lan,et al.Effects of IL-23 on the herin proliferation,invasion and bi-differentiation? of melanoma cells[J].Journal of Tianjin Medical University,2017,23(04):295-299.
点击复制

炎症因子IL-23 对黑色素瘤细胞 增殖侵袭及双向分化的影响(PDF)
分享到:

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23
期数:
2017年04期
页码:
295-299
栏目:
基础医学
出版日期:
2017-07-02

文章信息/Info

Title:
Effects of IL-23 on the herin proliferation,invasion and bi-differentiation? of melanoma cells
文章编号:
1006-8147(2017)04-0295-05
作者:
文明明1马跃美1赵秀兰23张丹芳2赵 楠2李岩磊2林 贤2董学易2刘 芳2
(1.天津医科大学外科手术学教研室,天津 300070;2.天津医科大学病理学教研室,天津 300070;3.天津医科大学总医院病理科,天津 300052 )
Author(s):
WEN Ming-ming1MA Yue-mei1 ZHAO Xiu-lan23 ZHANG Dan-fang2ZHAO Nan 2 LI Yan-lei2 LIN Xian2 DONG Xue-yi2 LIU Fang2
(1.Department of Operative Surgery, Tianjin Medical University, Tianjin 300070, China;2.Department of Pathology, Tianjin Medical University, Tianjin 300070, China; 3.Department of Pathology, General Hospital , Tianjin Medical University, Tianjin 300052, China)
关键词:
黑色素瘤IL-23增殖迁移侵袭
Keywords:
malignant melanomaIL-23 proliferationmigration invasion
分类号:
R36
DOI:
-
文献标志码:
A
摘要:
目的:检测白细胞介素-23(IL-23)在黑色素瘤中的表达并探讨IL-23 对黑色素瘤细胞双向分化及增殖、侵袭能力的影响。方法:将IL-23表达质粒转染至黑色素瘤细胞系MUM-2B中,使MUM-2B中IL-23外源性降表达,观察IL-23转染前、后MUM-2B细胞形态学改变;MTT、细胞划痕和Transwell侵袭实验检测对增殖、迁移及侵袭能力的影响;Western blot检测转染前、后MUM-2B中IL-23、相关蛋白(Vimentin、Snail、N-cadherin和MMP-2)表达变化情况,免疫荧光检测IL-23、Vimentin和MMP-2表达部位,明胶酶谱检测MMP-2,MMP-9活性。结果:转染后MUM-2B的细胞形态发生明显改变。细胞的增殖、迁移及侵袭能力显著减弱。IL-23降表达,N-cad、Vimentin、NF-κβ、Snail和MMP-2表达明显下调,MMP-2,MMP-9活性显著降低。结论:IL-23 可促进黑色素瘤细胞向间叶样表型转变,并促进黑色素瘤的增殖、迁移和侵袭。
Abstract:
Objective:To examine the expression of IL-23 in melanoma cells and its effecton proliferation,invasion and bi-differentiation.

Methods: To establish stable IL-23 knockdown cells, MUM-2B cells were stably transfected with sh-IL-23 and sh-Control vectors.The changes of MUM-2B cells morphology were detected after IL-23transfection by using microscope;The proliferation of MUM-2Bcells was determined by MTT,the migration ofMUM-2B cells was determined by the wound healing assay. And the invasion of MUM-2B cells was detected by Transwellexperiment.The changes of the expression of related protein(Vimentin,Snail,N-cadherin and MMP-2)in MUM-2Bcells were detected by Western blot after transfection ofIL-23;Using immunofluorescence, the sites of the expression of related protein(IL-23,Vimentin and MMP-2) were detected;The expression activities of MMP-2 and MMP-9 were analyzed by Gelatin Zymography. Results:After IL-23 knockdown, the morphology ofMUM-2B cells showed significant changes; IL-23 knockdown significantly decreased the abilities of MUM-2B cells to proliferate ,invade and migrate;Compared with MUM-2B-Control cells, knockdown expression of IL-23 significantly down-regulated N-cad, Vimentin, NF-κβ,Snailand MMP-2. Importantly, MUM-2B cells knockdown IL-23 demonstrated decreasedtheexpressionactivities of MMP-2 and MMP-9.Conclusion: IL-23 contributes to transfection of mesenchymal-like phenotypeand facilitates the proliferation,migration and invasionof melanoma cells.

参考文献/References:

[1] Caini S, Boniol M, Botteri E, et al. The risk of developing a second primary cancer in melanoma patients: a comprehensive review of the literature and meta-analysis[J]. J Dermatol Sci, 2014, 75(1): 3

[2] Zbytek B, Carlson JA, Granese J, et al. Current concepts of metastasis in melanoma[J]. Expert Rev Dermatol, 2008. 3(5): 569

[3] 杨举伦. 双向分化肿瘤的病理诊断[J]. 诊断病理学杂志, 1997, 4(3): 164

[4] Schreiber RD, Old LJ, Smyth MJ. Cancer immunoediting: integrating immunity’s roles in cancer suppression and promotion[J]. Science. 2011, 331(6024): 1565

[5] Feng S, Chen XM, Wang JF, et al. Th17 cells associated cytokines and cancer[J]. Eur Rev Med Pharmacol Sci, 2016, 20(19): 4032

[6] von SB, Leung PS, Yong MC, et al. Combined anti-CD40 and anti-IL-23 monoclonal antibody therapy effectively suppresses tumor growth and metastases[J]. Cancer Res, 2014, 74(9): 2412

[7] Lyakh L, Trinchieri G, Provezza L,et al. Regulation of interleukin-12/interleukin-23 production and the T-helper 17 response in humans[J]. Immunol Rev, 2008, 226: 112

[8] Boniface K, Blom B, Liu YJ, de Waal Malefyt R. From interleukin-23 to T-helper 17 cells: human T-helper cell differentiation revisited[J]. Immunol Rev, 2008, 226: 132

[9] 赵尔增, 岳茂兴. 肛管直肠原发性恶性黑色素瘤临床病理特点[J]. 世界华人消化杂志, 2004, 12(2): 225

[10] 纪小龙, 申明识. 黏膜黑色素瘤的常见临床病理特点[J]. 诊断病理学杂志, 2002 ,9 (2): 50

[11] Keibel A, Singh V, Sharma MC. Inflammation, microenvironment, and the immune system in cancer progression[J]. Curr Pharm Des, 2009, 15(17): 1949

[12] Atsumi T, Singh R, Sabharwal L, et al. Inflammation amplifier, a new paradigm in cancer biology[J]. Cancer Res, 2014, 74(1): 8

[13] Capece D, Fischietti M, Verzella D, et al. The inflammatory microenvironment in hepatocellular carcinoma: a pivotal role for tumor-associated macrophages[J]. Biomed Res Int, 2013, 2013: 187204

[14] Langowski JL, Zhang X, Wu L, et al. IL-23 promotes tumour incidence and growth[J]. Nature, 2006,442(7101): 461

[15] Zhang L, Li J, Li L, et al. IL-23 selectively promotes the metastasis of colorectal carcinoma cells with impaired Socs3 expression via the STAT5 pathway[J]. Carcinogenesis, 2014, 35(6): 1330

[16] Seftor RE, Seftor EA, Koshikawa N, et al. Cooperative interactions of laminin 5 gamma2 chain, matrix metalloproteinase-2, and membrane type-1-matrix/metalloproteinase are required for mimicry of embryonic vasculogenesis by aggressive melanoma[J]. Cancer Res, 2001, 61(17): 637



相似文献/References:

[1]田原,马跃美,刘艳荣,等.炎症因子IL-23对黑色素瘤B16F10细胞增殖和侵袭能力的影响[J].天津医科大学学报,2014,20(01):8.
 TIAN Yuan,MA Yue-mei,LIU Yan-rong,et al.Effects of IL-23 on proliferation and invasion of B16 melanoma cells[J].Journal of Tianjin Medical University,2014,20(04):8.

备注/Memo

备注/Memo:
基金项目 国家自然科学基金面上项目基金资助(81572872)

作者简介 文明明(1989-),男,硕士在读,研究方向:炎症与肿瘤血管生成关系的研究;

通信作者:马跃美,E-mail:maym@tmu.edu.cn

更新日期/Last Update: 2017-06-30