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[1]任梦萌,王伟占,方 涛,等.替米沙坦对胰岛素抵抗大鼠PPAR-?蛋白表达的影响[J].天津医科大学学报,2017,23(02):124-127.
 REN Meng-meng,WANG Wei-zhan,FANG Tao,et al.Effects of telmisartan on the expression of PPAR-? protein in OLETF rats with insulin resistance[J].Journal of Tianjin Medical University,2017,23(02):124-127.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年02期
页码:
124-127
栏目:
基础医学
出版日期:
2017-03-20

文章信息/Info

Title:
Effects of telmisartan on the expression of PPAR-? protein in OLETF rats with insulin resistance
文章编号:
1006-8147(2017)02-0124-04
作者:
任梦萌12王伟占2方 涛3田凤石2
(1.天津医科大学研究生院,天津 300070;2.天津市第四中心医院心内科,天津 300140;3. 天津市第四中心医院中心实验室,天津 300140)
Author(s):
REN Meng-meng12WANG Wei-zhan2 FANG Tao3 TIAN Feng-shi2
(1.Graduate School, Tianjin Medical University, Tianjin 300070, China; 2. Department of Cardiology, The Fourth Center Hospital of Tianjin, Tianjin 300140, China; 3. Center Laboratory, The Fourth Center Hospital of Tianjin, Tianjin 300140, China)
关键词:
胰岛素抵抗替米沙坦吡格列酮过氧化物酶体增殖物激活受体? 大鼠
Keywords:
insulin resistance telmisartan pioglitazone peroxisomerat proliferators-activated receptor ? yat
分类号:
R58
DOI:
-
文献标志码:
A
摘要:
目的: 观察替米沙坦对胰岛素抵抗(IR)大鼠肝脏、肾周脂肪和骨骼肌组织PPAR?蛋白表达的影响。方法: 雄性OLETF大鼠40只,高脂饲养20周建立IR模型后,随机分为4组(n=10只),即高脂对照组(O)、吡格列酮组(P)、低剂量替米沙坦组(VT)和替米沙坦组(T),进行药物干预,O组单纯高脂喂养,P组给予高脂加吡格列酮,VT组给予高脂加低剂量替米沙坦,T组给予高脂加替米沙坦。LETO大鼠12只为NC(正常对照)组,单纯予标准饲料。干预20周后测定各组大鼠FBG、FINS、HOMA-IR、ISI、TC、TG、HDL-C、LDL-C水平。Western blot测定各组织PPAR-?蛋白表达。结果: 40只OLETF大鼠均出现IR,造模成功。干预20周后,O组 FBG、 FINS、 HOMAIR、 TC、 TG、和LDL-C水平均高于P、VT和T组, ISI、 HDL-C均低于P、VT和T组,根据Western结果可看出P、VT、T组所取各组织PPAR-?蛋白表达均高于O组(均P<0.05);VT组 FBG、 FINS、 HOMAIR、 TC、 TG、和LDL-C水平均高于T组,ISI、 HDL-C水平低于T组,且T组比VT组各组织PPAR-?蛋白表达较高(均 P<0.05)。结论: 替米沙坦可通过上调肝脏、肾周脂肪及骨骼肌组织PPAR-?蛋白的表达来改善IR,且改善程度可能与替米沙坦剂量有关。
Abstract:
Objective: To observe the effects of telmisartan on the expression of PPAR-? protein in rats tissues including liver, muscle and perirenal fat with IR. Methods: Forty male OLETF rats were fed with high-fat diet for 20 weeks to establish the IR model, then randomly assigned into four groups: the high-fat diet set(O) were given pure high-fat diet, the Pioglitazone group (P) were fed with high-fat diet and Pioglitazone, the low dose Telmisartan group (VT) were fed with high-fat diet and low dose Telmisartan, and rats in the Telmisartan group (T) were fed with high-fat diet and Telmisartan. Twelve LETO rats raised by standard feeding served as normal control group (NC). Following 6 weeks-drug intervention, the plasma levels of FBG, FINS, HOMA-IR, ISI, TC, TG, HDL-C, LDL-C were detected in four groups, and the expression level of PPAR-? protein was measured using Western blot in each tissues . Results: Insulin resistance was observed in all 40 rats and buildings were established. Compared with group P, VT and T, the FBG, FINS, HOMA-IR, TC, TG, and LDL-C in O group were significantly increased. ISI and HDL-C were decreased (P <0.05). The expressions of PPAR? protein in group P, VT and T were higher than group O. And the group T was lower than VT group in biochemical index results except for ISI and HDL-C; and the expression of PPAR? protein was higher. Conclusion: Telmisartan can significantly improve IR through increasing the expression of PPAR-? protein in liver, perirenal fat and skeletal muscle tissues; and the improvement degree may be associated with telmisartan dose.

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备注/Memo

备注/Memo:
基金项目 天津市应用基础与前沿技术研究计划(青年项目)基金资助(15JCQNJC13100)
作者简介 任梦萌(1990-),女,硕士在读,研究方向:心血管内科;

通信作者:田凤石,Email:fengshitian0801@hotmail.com

更新日期/Last Update: 2017-03-28