|本期目录/Table of Contents|

[1]张 洁,张 飞,冀 为,等. SHP2不同突变体对乳腺癌细胞的迁移和侵袭能力的影响[J].天津医科大学学报,2015,21(02):93-96.
 ZHANG Jie,ZHANG Fei,JI Wei,et al. Effect of different SHP2 mutants on breast cancer cell migration and invasion[J].Journal of Tianjin Medical University,2015,21(02):93-96.
点击复制

 SHP2不同突变体对乳腺癌细胞的迁移和侵袭能力的影响(PDF)
分享到:

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21
期数:
2015年02期
页码:
93-96
栏目:
基础医学
出版日期:
2015-03-20

文章信息/Info

Title:
 Effect of different SHP2 mutants on breast cancer cell migration and invasion
文章编号:
1006-8147(2015)02-0093-04
作者:
 张 洁张 飞冀 为赵元元牛瑞芳
 (天津医科大学肿瘤医院公共实验室,国家肿瘤临床医学研究中心,天津市“肿瘤防治”重点实验室,天津 300060)
Author(s):
 ZHANG Jie ZHANG Fei JI Wei ZHAO Yuan-yuan NIU Rui-fang
 (Public Laboratory, Cancer Institute and Hospital, Tianjin Medical University ,National Clinical Research Center of Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China)

关键词:
乳腺癌 SHP2 磷酸酶活性迁移侵袭
Keywords:
 breast cancer SHP2 phosphatase activity invasion migration

分类号:
R737.9
DOI:
-
文献标志码:
A
摘要:
 目的:采用慢病毒感染的方式将SHP2基因和SHP2突变体导入乳腺癌细胞系MDA-MB-231中, 研究SHP2及其各突变体对乳腺癌细胞迁移和侵袭能力的影响。方法:构建SHP2-WT和SHP2-T468M、SHP2-N308D和SHP2-2YF 3个突变体的慢病毒载体,完成慢病毒包装后,感染乳腺癌细胞系MDA-MB-231稳定表达。通过蛋白免疫印记检测高表达情况,免疫荧光观察定位情况,划痕、Transwell实验检测细胞的迁移和侵袭能力。结果:SHP2-WT增强了乳腺癌细胞迁移和侵袭能力,SHP2-T468M,SHP2-N308D与对照相比无显著影响,SHP2-2YF与对照相比降低了乳腺癌细胞的迁移和侵袭能力。结论:SHP2-WT能显著增强乳腺癌细胞迁移和侵袭能力,这可能与其磷酸酶活性无关,而与其542位和580酪氨酸的磷酸化有关。
Abstract:
Objective: To explore the effects of SHP2 and its mutants on migration and invasion of breast cancer cells by transfecting SHP2 and SHP2 mutants into breast cancer cell line MDA-MB-231. Methods: First inglentiviral vector SHP2-WT and three mutants of SHP2-T468M, SHP2-N308D and SHP2-2YF were constructed, and  Then the lentiviral packaging was completed to infect breast cancer cell line MDA-MB-231 for stable expression. The expression, location, cell migration and invasion were detected by western blotting, immunofluorescence, wound-healing and transwell assay respectively. Results: Migration and invasion abilities breast cancer cell of were enhanced by SHP2-WT. SHP2-T468M, SHP2-N308D had no significant effect compared with the control group. The SHP2-2YF lessened the migration and invasion in breast cancer cells. Conclusion: SHP2-WT can significantly increase the invasion and migration of breast cancer cells, which may not be related to the phosphatase activity, but associate with 542 and 580 tyrosine phosphorylation .

参考文献/References:

[1]Jemal A, Bray F, Center M M, et al. Global cancer statistics[J].CA Cancer J Clin, 2011,61(2):69

[2]Chan G, Kalaitzidis D, Neel B G. The tyrosine phosphatase Shp2 (PTPN11) in cancer[J]. Cancer Metastasis Rev,2008,27(2):179

[3]Dance M, Montagner A, Salles J P, et al. The molecular functions of Shp2 in the Ras/Mitogen-activated protein kinase (ERK1/2) pathway[J].Cell Signal,2008,20(3):453

[4]Grossmann K S, Rosário M, Birchmeier C, et al.The tyrosine phosphatase Shp2 in development and Cancer[J].Adv Cancer Res,2010,106:53

[5]Aceto N, Sausgruber N, Brinkhaus H, et al. Tyrosine phosphatase SHP2 promotes breast cancer progression and maintains tumor-initiating cells via activation of key transcription factors and a positive feedback signaling loop[J].Nat Med,2012,18(4):529

[6]Hartman Z R, Schaller M D, Agazie Y M. The tyrosine phosphatase SHP2 regulates focal adhesion kinase to promote EGF-induced lamellipodia persistence and cell migration[J].Mol Cancer Res,2013,11(6):651

[7]Tidyman W E, Rauen K A. The RAS opathies: developmental syndromes of Ras/MAPK pathway dysregulation [J].CurrOpin Genet Dev,2009,19(3):230

[8]Tartaglia M, Gelb B D. Disorders of dysregulated signal traffic through the RAS-MAPK pathway: phenotypic spectrum and molecular mechanisms[J].Ann N Y Acad Sci,2010,1214:99

[9]Araki T, Nawa H, Neel B G.T yrosyl phosphorylation of Shp2 is required for normal ERK activation in response to some, but not all, growth factors[J].J Biol Chem,2003,278(43):41677

[10]Cleghon V, Feldmann P, Ghiglione C, et al. Opposing actions of CSW and RasGAP modulate the strength of Torso RTK signaling in the Drosophila terminal pathway[J].Mol Cell,1998,2(6):719

[11]Miura K, Wakayama Y, Tanino M, et al. Involvement of EphA2-mediated tyrosine phosphorylation of Shp2 in Shp2-regulated activation of extracellular signal-regulated kinase[J].Oncogene,2013,32(45):5292

相似文献/References:

[1]朱悦,张诗武,张丹芳,等.TA2小鼠自发乳腺癌血清蛋白质组学研究[J].天津医科大学学报,2013,19(05):373.
[2]刘 营,孙保存,刘铁菊,等.AURKA蛋白激酶在三阴乳腺癌干细胞形成血管拟态中的实验研究[J].天津医科大学学报,2013,19(06):437.
 LIU Ying,SUN Bao-cun,LIU Tie-ju,et al.Experimental study of AURKA protein kinase in the formation of vascular mimicry in triple-negative breast cancer stem cells[J].Journal of Tianjin Medical University,2013,19(02):437.
[3]伦淑敏.HOXA5基因真核表达质粒的构建及在乳腺癌细胞中的功能研究[J].天津医科大学学报,2014,20(05):337.
 LUN Shu-min. Construction of HOXA5 eukaryotic expression plasmid of and its biological significance in breast cancer cells[J].Journal of Tianjin Medical University,2014,20(02):337.
[4]伦淑敏.肌细胞增强因子2A基因真核表达质粒的构建及对乳腺癌细胞MCF-7增殖能力的影响[J].天津医科大学学报,2014,20(06):429.
 LUN Shu-min.Construction of myocyte enhancer factor 2A eukaryotic expression plasmid and effects on cell proliferation in breast cancer cell line MCF7[J].Journal of Tianjin Medical University,2014,20(02):429.
[5]孙秀梅,张 飞,田 然,等.Nanog表达上调促进乳腺癌细胞MCF-7的增殖和侵袭[J].天津医科大学学报,2014,20(06):421.
 SUN Xiu-mei,ZHANG Fei,TIAN Ran,et al.Up-regulation of Nanog promotes cell proliferation and invasion in breast cancer cells MCF-7[J].Journal of Tianjin Medical University,2014,20(02):421.
[6]蔡 隽. FOXQ1稳定表达乳腺癌细胞系的建立及鉴定[J].天津医科大学学报,2015,21(04):292.
 CAI Jun.Establishment and identification of cell lines with stable expression of FOXQ1 in MDA-MB-231-luc[J].Journal of Tianjin Medical University,2015,21(02):292.
[7]蔡 隽 综述,冯玉梅 审校.叉头框转录因子调控乳腺癌生物学特性的研究进展[J].天津医科大学学报,2015,21(05):455.
[8]任宗娜.沉默Notch4基因对乳腺癌细胞系MDA-MB-231增殖和迁移侵袭能力的影响[J].天津医科大学学报,2015,21(06):469.
 REN Zong-na.Inhibition effect of?silencing?? Notch4 gene on the proliferation and migration and invasion activity of? breast cancer cell line?MDA-MB-231[J].Journal of Tianjin Medical University,2015,21(02):469.
[9]周岩,宋伟杰,张飞,等.人附睾蛋白4在乳腺癌发生发展中的机制研究[J].天津医科大学学报,2015,21(06):466.
 ZHOU Yan,SONG Wei-jie,ZHANG Fei,et al.Mechanism of human epididymis protein 4 in development and progression of breast cancer[J].Journal of Tianjin Medical University,2015,21(02):466.
[10]刘晓丽,黄 勇,杨丽敏,等.二氢嘧啶脱氢酶基因的T85C和A1627G联合突变对乳腺癌患者预后的影响[J].天津医科大学学报,2016,22(05):412.
 LIU Xiao-li,HUANG Yong,YANG Li-min,et al.Effect of combined mutations of T85C and A1627G in dihydropyrimidine dehydrogenase gene on prognosis of patients with breast cancer[J].Journal of Tianjin Medical University,2016,22(02):412.

备注/Memo

备注/Memo:
 基金项目 国家自然科学基金资助项目(81372844);教育部博士点基金资助项目(20131202110002);天津医科大学科研基金资助项目(2009ky21)

作者简介 张洁(1989-),女,硕士在读,研究方向:生物化学与分子生物学;

通信作者:牛瑞芳,E-mail:niuruifang@tjmuch.com

更新日期/Last Update: 2015-03-24