|本期目录/Table of Contents|

[1]姚 远,景换利,高卫平.毛细管电泳差异性检测尿液蛋白方法初探[J].天津医科大学学报,2016,22(04):359-363.
　YAO Yuan,JING Huan-li,GAO Wei-ping.Study?on the difference detection method? for urinary proteomic by CE-DAD technology[J].Journal of Tianjin Medical University,2016,22(04):359-363.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
22卷
期数:
2016年04期
页码:
359-363
栏目:
技术与方法
出版日期:
2016-07-19

文章信息/Info

Title:
Study?on the difference detection method? for urinary proteomic by CE-DAD technology
文章编号:
1006-8147（2016）04-0359-05
作者:
姚 远;  景换利; 高卫平
( 天津医科大学生物医学工程学院, 天津 300070)
Author(s):

YAO Yuan;  JING Huan-li;  GAO Wei-ping

(College of Biomedical Engineering, Tianjin Medical University, Tianjin, 300070, China )
关键词:
毛细管电泳; 尿液蛋白; 差异检测; 电渗流?
Keywords:
capillary electrophoresis;  urinary proteomic;  difference detection;  electro-osmotic flow
分类号:
R318.6
DOI:
-
文献标志码:
A
摘要:
?目的：使用毛细管电泳(CE)与二极管阵列检测(DAD)联用技术(CE-DAD)的差异检测方法，优化膀胱癌患者尿液特异性蛋白的检测条件。方法：对5例膀胱癌患者及5例健康志愿者尿液蛋白中的低丰度蛋白进行含量的差异检测，并使用电解质添加剂对原缓冲体系进行了优化，对电渗流进行有效控制，提高低丰度蛋白的分离效率。结果：确立了使用毛细管电泳与二极管阵列检测联用技术对正常人组与非肌层浸润性膀胱癌患组尿液蛋白的差异检测最佳条件，并经ELISA法平行试验，显示两组低丰度蛋白存在差异相关性。结论：对CE-DAD的差异检测条件进行了初步的探索，为未来标准化检测方法的建立奠定了基础。
Abstract:
Objective: To explore an optimized method for difference detection of urinary proteomics in patients with Bladder Cancer (BCs) by Capillary Electrophoresis separation technique coupled with diode array detector (CE-DAD). Methods: For detecting the difference in the contents of each urinary low-abundance proteins between the two groups, electrolytic additives were used to improve the buffer system, and the concentration of electro-osmotic flow was well controlled in this condition, in order to increase the separation efficiency. Results: The optimal conditions were established to detect the difference between the bladder cancer and the control group by CE-DAD, and the difference and correlation were also tested by the parallel experiment of ELISA method. Conclusion: This paper makes a preliminary exploration on the condition of difference detection by CE-DAD, and establishes the foundation for the institution of standardized experimental method in the future.

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相似文献/References:

[1]宋 珑,范清杰,包日煌,等.毛细管电泳-发光二极管诱导荧光分析尿液蛋白[J].天津医科大学学报,2014,20(05):404.
　SONG Long,FAN Qing-jie,BAO Ri-huang,et al.Analyzing urine proteins by capillary electrophoresis combined with light-emitting diode induced fluorescence[J].Journal of Tianjin Medical University,2014,20(04):404.
[2]景换利,姚 远,高卫平.应用高效毛细管电泳法测定2型糖尿病肾病患者尿微量白蛋白的含量[J].天津医科大学学报,2017,23(05):475.
　JING Huan-li,YAO Yuan,GAO Wei-ping.A simple determination of microalbuminuria urine of patients with type 2 diabetic nephropathy by capillary electrophoresis[J].Journal of Tianjin Medical University,2017,23(04):475.

备注/Memo

备注/Memo:

作者简介 姚远（1991-），男，硕士在读，研究方向：医学仪器；通信作者：高卫平，E-mail: gaoweiping@tijmu.edu.cn。

更新日期/Last Update: 2016-07-13