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[1]景换利,姚 远,高卫平.应用高效毛细管电泳法测定2型糖尿病肾病患者尿微量白蛋白的含量[J].天津医科大学学报,2017,23(05):475-479.
 JING Huan-li,YAO Yuan,GAO Wei-ping.A simple determination of microalbuminuria urine of patients with type 2 diabetic nephropathy by capillary electrophoresis[J].Journal of Tianjin Medical University,2017,23(05):475-479.
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应用高效毛细管电泳法测定2型糖尿病肾病患者尿微量白蛋白的含量(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23
期数:
2017年05期
页码:
475-479
栏目:
技术与方法
出版日期:
2017-09-20

文章信息/Info

Title:
A simple determination of microalbuminuria urine of patients with type 2 diabetic nephropathy by capillary electrophoresis
文章编号:
1006-8147(2017)05-0475-05
作者:
景换利姚 远高卫平
(天津医科大学生物医学工程与技术学院,天津300070)
Author(s):
JING Huan-li YAO YuanGAO Wei-ping
(College of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, China)
关键词:
毛细管电泳2型糖尿病肾病尿微量白蛋白
Keywords:
capillary electrophoresis type 2 diabetes nephropathy microalbuminuria
分类号:
R318.6
DOI:
-
文献标志码:
A
摘要:
目的:应用毛细管电泳耦合二极管阵列检测器(CE-DAD)技术检测2型糖尿病肾病患者尿微量白蛋白(mALB)的含量。方法:实验过程中,对缓冲液的浓度、pH值、分离电压、最佳波长以及添加剂十二烷基硫酸钠(SDS)的浓度进行系统的研究,得到了最佳分离条件,即运行缓冲溶液为10 mmol/L硼酸盐+70 mmol/L SDS,pH=9.25, 检测波长194 nm, 分离电压15 kV, 分离温度25 ℃的条件下,通过毛细管电泳压力进样8 s, 使空白对照组、糖尿病组和肾病组的mALB在15 min内均被完全分离。结果:糖尿病肾病组患者中mALB的含量均高于健康对照组,差异有显著统计学意义,并且随着患者病情的加重,患者体内的mALB的含量明显增高。结论:应用CE-DAD检测方法能够实现mALB良好分离,是一种可靠、高效的分离方法。
Abstract:
Objective: To establish a new method for determination of the microalbuminuria (mALB) in urine of patients with type 2 diabetic nephropathy (T2DN) by capillary electrophoresis (CE) coupled with diode array detector (DAD).Methods: Experimental parameters such as concentration and pH of buffer solution, the separation voltage, optimal wavelength and SDS concentration were systematically investigated. The optimum separation conditions were determined as: 10 mmol/L borate buffer and 70 mmol/L SDS at pH 9.25 with 8 s hydrodynamic injection at 50 mbar, optimal wavelength 194 nm,applied voltage were set at 15 kV and working temperature at 25 ℃, and the mALB were completely separated within 15 min. Results: There was a significantly difference between the healthy control and the patients, and the concentration of mALB had significantly increased as the disease became worse. Conclusion: This CE-DAD method can achieve good accuracy and repetition for analyzing mALB.

参考文献/References:

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[8]Christians S, van Treel N D, Bieniara G, et al. Evaluation of capillary zone electrophoresis for the determination of protein composition in therapeutic immunoglobulins and human albumins[J].Biologicals,2016, 44(4):234

[9]Tian Y, Li Y, Mei J, et al.Simultaneous separation of acidic and basic proteins using gemini pyrrolidinium surfactants and hexafluoroiso- propanol as dynamic coating additives in capillary electrophore- sis[J].Journal of Chromatogr A, 2015,1412(18):151

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[2]姚 远,景换利,高卫平.毛细管电泳差异性检测尿液蛋白方法初探[J].天津医科大学学报,2016,22(04):359.
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备注/Memo

备注/Memo:
作者简介 景换利(1989-),女,硕士在读,研究方向:分析化学毛细管电泳;

通信作者:高卫平,E-mail:weipinggao88@126.com

更新日期/Last Update: 2017-09-21