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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年05期

页码:

404-407

栏目:

技术与方法

出版日期:

2014-09-20

文章信息/Info

Title:

Analyzing urine proteins by capillary electrophoresis combined with light-emitting diode induced fluorescence

文章编号:

1006-8147(2014)05-0404-04

作者:

宋 珑¹; 范清杰²; 包日煌¹; 姚 远¹; 高卫平¹

(1.天津医科大学生物医学工程,天津300070; 2. 天津市兰力科化学电子高技术有限公司,天津 300384）

Author(s):

SONG Long¹;  FAN Qing-jie²;  BAO Ri-huang¹;  YAO Yuan¹;  GAO Wei-ping¹

(1. Department of Biomedical Engineering，Tianjin Medical University, Tianjin 300070, China; 2. Tianjin Lanlike Chemistry and Electron High Technology Co. , Ltd. , Tianjin 300384, China)

关键词:

 ; 毛细管电泳; 发光二极管; 诱导荧光; 尿液蛋白质

Keywords:

capillary electrophoresis;  light-emitting diode;  induced fluorescence;  urine proteins

分类号:

R318.04

DOI:

-

文献标志码:

A

摘要:

目的：建立人体尿液蛋白质毛细管电泳分析方法，检测正常人与膀胱癌患者尿液蛋白质成分的差异性。方法：对正常人与膀胱癌患者尿液蛋白质用异硫氰酸荧光素进行荧光标记，进行毛细管电泳-发光二极管诱导荧光法分析，获得人尿液蛋白质电泳图，比较两种电泳图的差异。结果：建立了人体尿液蛋白质毛细管电泳分析方法，成功将正常人与膀胱癌患者尿液中的多种蛋白质分离并得到相应电泳图谱，正常人与膀胱癌患者尿液蛋白质电泳图谱存在差异。结论：毛细管电泳-发光二极管诱导荧光法能检测到正常人与膀胱癌患者尿液蛋白质的差异。

Abstract:

Objective: To study the value of capillary electrophoresis combined with light-emitting diode induced fluorescence detecting to detect the difference between normal human protein and bladder cancer urine protein. Methods: Proteins in normal and bladder cancer urine were labeled using fluorescein isothiocyanate and analyzed by capillary electrophoresis. Optimum conditions were developed. Electrophoretograms were applied. Results: Capillary electrophoresis was successfully applied to human urine proteins. Series of proteins in both normal and bladder cancer urine were detected and electrophoretograms were achieved simultaneously. Significant distinctions between normal and bladder cancer were found in the electrophoretograms. Conclusion: This method can detect the difference between normal protein and bladder cancer protein. Capillary electrophoresis combined with light-emitting diode induced fluorescence may be a useful method for urine protein analysis.

参考文献/References:

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备注/Memo

备注/Memo:

作者简介 宋珑（1988-），男，研士在读，研究方向：医学仪器；通信作者：高卫平，E-mail： weipinggao88@126.com。

常用功能

更新日期/Last Update: 2014-09-25