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[1]薄志强,孙桂江,王 丽,等.CtBP2异常表达对前列腺癌PC3细胞增殖效应影响的研究[J].天津医科大学学报,2017,23(02):103-107.
　BO Zhi-qiang,SUNGui-jiang,WANG Li,et al.Effect of abnormal expression of CtBP2 on the proliferation of prostate cancer cell line PC3[J].Journal of Tianjin Medical University,2017,23(02):103-107.
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CtBP2异常表达对前列腺癌PC3细胞增殖效应影响的研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年02期
页码:
103-107
栏目:
专题报道
出版日期:
2017-03-20

文章信息/Info

Title:
Effect of abnormal expression of CtBP2 on the proliferation of prostate cancer cell line PC3
文章编号:
1006-8147(2017)02-0103-05
作者:
薄志强1; 孙桂江2; 王 丽3; 张昌文1; 张志宏1
(天津医科大学第二医院1.泌尿外科，天津市泌尿外科研究所；2.血液透析中心；3.产科，天津300211）
Author(s):
BO Zhi-qiang; SUNGui-jiang; WANG Li; ZHANG Chang-wen; ZHANG Zhi-hong
(1.Department of Urology, The Second Hospital，Tianjin Medical University, Urology Research Institute of Tianjin；2.Hemodialysis Center；3.Department of Obstetrics , The Second Hospital ,Tianjin Medical University,Tianjin 300211,China)
关键词:
前列腺癌; CtBP2; 细胞增殖
Keywords:
prostate cancer; CtBP2; cell proliferation
分类号:
R737.25
DOI:
-
文献标志码:
A
摘要:
目的：探讨CtBP2在前列腺癌中的表达情况，并明确其异常表达对前列腺癌PC3细胞增殖效应的影响。方法：采用免疫组化、qPCR及Western blot检测CtBP2的表达情况，采用细胞计数法、单克隆集落形成实验及MTT法检测细胞的增殖能力。结果：在前列腺癌组织中CtBP2 蛋白高表达率为97.5%（156/160），表达水平较良性前列腺增生组织显著升高（P＜0.05），细胞计数及MTT实验结果表明CtBP2 Silencer组较Vector control组及Parent control组，其增殖能力降低分别为70%及75%（P＜0.05），单克隆集落形成实验结果显示Blank Control 组、Vector control组和Silencer组PC3细胞培养12 d后可见克隆数分别为（136±11）、（128±12）和（56±9）（P＜0.05），克隆面积为（342±22）mm2、（322±23）mm2和（122±16）mm2（P＜0.05）。结论：CtBP2在前列腺癌中高表达,而减低其表达水平可显著抑制前列腺癌细胞增殖能力。
Abstract:
Objective: To determine the expression of the CtBP2 in prostate cancer, and to explore the effect of the abnormal expressed CtBP2 on the proliferation of PC3 cells. Methods: The immunohistochemistry, qPCR and Western blot were used to assess the level of CtBP2.And MTT, cell number counting and cell clony formation assay were used to investigate the proliferation of PC3 cells. Results: CtBP2 was expressed in 97.5% prostate cancer tissues, and it was highly expressed compared with the adjacent normal prostate tissues (P＜0.05＝. MTT and cell number counting showed that cell proliferation was significantly inhibited by 75% and 70% in CtBP2-silenced cells compared with control, respectively. The colony formation assay showed that the numbers of clone in Blank control group, Vector control group and Silencer group were（136±11）、（128±12）and （56±9）（P＜0.05＝，and the areas of clone were（342±22）mm2、（322±23） mm2 and （122±16）mm2（P＜0.05＝. Conclusion: CtBP2 may be overexpressed in prostate cancers, and decreased expression of CtBP2 could inhibit the proliferation of PC3 cells.

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（81402124,81472416,21577097）；中国科学院战略性先导科技专项

资助项目（XDB14040401,XDB14010300）；天津市应用基础与前沿技术研究计划（14JCQNJC10800）；

天津市高等学校科技发展基金计划专项（20130124）

作者简介 薄志强（1990-），硕士在读，研究方向：泌尿外科；通信作者：张志宏，E-mail:zhihongzhang19667@163.com。



更新日期/Last Update: 2017-03-28