[1]马晓芳,童静凯,牛文彦.肥胖和缺氧对小鼠原代脂肪细胞分泌TNFα的影响[J].天津医科大学学报,2014,20(01):1-003.
　MA Xiao-fang,TONG Jing-kai,NIU Wen-yan.Effect of obesity and hypoxia on TNFa secretion by primary adipocytes in mice[J].Journal of Tianjin Medical University,2014,20(01):1-003.

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肥胖和缺氧对小鼠原代脂肪细胞分泌TNFα的影响(PDF)

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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年01期

页码:

1-003

栏目:

基础医学

出版日期:

2014-01-20

文章信息/Info

Title:

Effect of obesity and hypoxia on TNFa secretion by primary adipocytes in mice

文章编号:

1006-8147(2014)01-0001-03

作者:

马晓芳; 童静凯; 牛文彦

(天津医科大学免疫学系, 天津 300070)

Author(s):

MA Xiao-fang;  TONG Jing-kai;  NIU Wen-yan

(Department of Immunology, Tianjin Medical University , Tianjin 300070, China)

关键词:

肥胖; 脂肪细胞; 缺氧; 肿瘤坏死因子a; 炎症; 胰岛素抵抗; 小鼠

Keywords:

obesity;  adipocyte;  hypoxia;  TNFa;  inflammation;  insulin resistance;  mouse

分类号:

R398

DOI:

-

文献标志码:

A

摘要:

目的：常氧和缺氧培养正常和肥胖小鼠的原代脂肪细胞，比较其分泌的肿瘤坏死因子α（TNFα）水平，探讨缺氧在脂肪组织慢性炎症中的作用。方法：高脂及普通饮食喂养4周龄C57BL/6雄性小鼠16周，测量体质量、葡萄糖耐量、胰岛素耐量；处死小鼠后取睾周脂肪组织，提取原代脂肪细胞，常氧或缺氧培养24 h后取上清，ELISA法测定培养基中TNFα的浓度。结果：高脂饮食喂养造成小鼠肥胖和胰岛素抵抗。常氧培养的肥胖小鼠脂肪细胞较正常小鼠的脂肪细胞分泌更多的TNFα；缺氧孵育比常氧孵育的正常小鼠脂肪细胞分泌更多的TNFα；但缺氧不进一步增加肥胖小鼠脂肪细胞分泌TNFα。结论：缺氧和肥胖均上调小鼠原代脂肪细胞TNFα的分泌，但缺氧不影响肥胖小鼠脂肪细胞分泌TNFα，提示肥胖小鼠脂肪细胞缺氧与其慢性炎症及胰岛素抵抗有关。

Abstract:

Objective: To explore the effect of hypoxia on adipose tissue inflammation by culturing primary adipocytes from lean and obese mice under normoxia or hypoxia condition and measuring the TNFα levels in the culture medium (conditioned dedium, CM). Methods: 4-week old male C57BL/6 male mice were fed with high fat diet (HFD) or chow diet (LFD) for 16 weeks, then body weight，intraperitoneal glucose tolerance test (ipGTT) and insulin tolerance test (ITT) were performed. The epididymal fat pads were taken and primary adipocytes were isolated and cultured under normoxic or hypoxic condition for 24 h. The levels of tumor necrosis factor-α ( TNFα) in CMs were determined by ELISA. Results: HFD increased body weight and caused insulin resistance. Adipocytes in obese mice secreted higher level of TNFα than normal mice. Hypoxia treatment increased level of TNFα secreted by adipocytes in normal mice. However, hypoxia did not affect TNFα secretion ?in obese mice. Conclusion: Both hypoxia and obese up-regulate TNFα secretion from murine primary adipocytes, but hypoxia does not further increase TNFα secretion from adipocytes of obese mice.

参考文献/References:

[1].Saquib N, Khanam M A, Saquib J, et al. High prevalence of type 2 diabetes among the urban middle class in Bangladesh[J]. BMC Public Health, 2013, 13(1):1032
[2].Sears D D, Miles P D, Chapman J, et al. 12/15-lipoxygenase is required for the early onset of high fat diet-induced adipose tissue inflammation and insulin resistance in mice[J]. PLoS One, 2009,4(9): e7250

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备注/Memo

备注/Memo:

 (基金项目 国家自然科学基金面上项目（81170740）；国家自然科学基金委国际合作项目（81161120545）；高等学校博士学科点专项基金（20121202110014）

作者简介 马晓芳（1987-），女，硕士在读；通信作者：牛文彦，E-mail:wniu@tijmu.edu.cn.

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