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[1]叶舒婷,杜湧瑞,王建梅.维生素D干预对高盐高脂高糖饮食雄性小鼠的子代生育能力的影响[J].天津医科大学学报,2023,29(04):413-418.
　YE Shu-ting,DU Yong-rui,WANG Jian-mei.Effect of vitamin D intervention on offspring fertility in male mice on a high-salt，high-fat，high-sugar diet[J].Journal of Tianjin Medical University,2023,29(04):413-418.
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维生素D干预对高盐高脂高糖饮食雄性小鼠的子代生育能力的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
29卷
期数:
2023年04期
页码:
413-418
栏目:
基础医学
出版日期:
2023-07-10

文章信息/Info

Title:
Effect of vitamin D intervention on offspring fertility in male mice on a high-salt，high-fat，high-sugar diet
文章编号:
1006-8147（2023）04-0413-05
作者:
叶舒婷; 杜湧瑞; 王建梅
天津医科大学第二医院计划生育科，天津 300211
Author(s):
YE Shu-ting; DU Yong-rui; WANG Jian-mei
Department of Family Planning，The Second Hospital，Tianjin Medical University，Tianjin 300211，China
关键词:
维生素D; 高盐高脂高盐饮食; 肥胖; 雄性生殖
Keywords:
vitamin D; high-salt; high-fat; high-sugar diet; obesity; male reproduction
分类号:
R711.6
DOI:
-
文献标志码:
A
摘要:
目的：探讨维生素D干预对高盐高脂高糖饮食雄鼠子代生育能力的作用。方法：C57BL/6j雄鼠随机分为3组：对照饮食组（CD）、高盐高脂高糖饮食组（HFSSD）、高盐高脂高糖饮食+维生素D组（HFSSD+D），喂养9周。不同饮食组雄鼠分别与对照饮食雌鼠交配，得到子代雄鼠，对照饮食喂养至8周龄，分别评估父系和子代雄鼠体重、生育能力、精子参数和睾丸形态学变化，统计并分析结果。结果：父系雄鼠HFSSD组4、5周龄体重与CD组相比差异无统计学意义，其他周龄雄鼠的体重均高于CD组（均P<0.05），HFSSD组4、6、7、8周龄体重高于HFSSD+D组（均P<0.05），其他周龄雄鼠的体重与HFSSD+D组相比无统计学意义。HFSSD组的子代雄鼠5～8周龄体重均高于CD组（均P<0.05），与HFSSD+D组相比差异无统计学意义。父系和子代HFSSD组雄鼠精子总数、精子活力均低于CD组和HFSSD+D组（均P<0.05），精子畸形率高于CD组（P<0.05），但与HFSSD+D组相比差异无统计学意义。父系HFSSD组雌鼠受孕率和每窝产仔数均低于CD组和HFSSD+D组（均P<0.05）。子代HFSSD组雌鼠受孕率和每窝产仔数均低于CD组（均P<0.05），但与HFSSD+D组相比差异无统计学意义。与CD组相比，父系和子代HFSSD组雄鼠睾丸生精小管管壁变薄，细胞间排列松散、紊乱；HFSSD+D组雄鼠睾丸生精小管管壁比HFSSD组细胞层数多，父系HFSSD+D组细胞间排列紊乱减少，子代HFSSD+D组未见紊乱。结论：维生素D干预可提高高盐高脂高糖饮食雄鼠子代精子质量，对子代生育能力具有潜在的改善作用，对优生优育具有指导意义。
Abstract:
Objective：To investigate the effect of vitamin D intervention on the fertility of male rat offspring on a high-salt，high-fat， high-sugar diet. Methods：Male C57BL/6j rats were randomly divided into three groups：control diet group（ CD），high salt，high fat and high sugar diet group（ HFSSD），and high salt，high fat and high sugar diet+vitamin D group（ HFSSD+D），and fed for 9 weeks. Male rats in the different diet groups were mated with females on the control diet to obtain offspring males，which were fed on the control diet until 8 weeks of age. Body weight，fertility，sperm parameters and testicular morphological changes were assessed in the sire and offspring males， respectively，and the results were counted and analyzed. Results：Body weights at 4 and 5 weeks of age were not statistically significant in the HFSSD group compared with the CD group for sire males，but were higher in all other weeks of age（ all P<0.05），and were higher in the HFSSD group at 4，6，7 and 8 weeks of age compared with the HFSSD+D group（ all P<0.05），but were not statistically significant in other weeks of age compared with the HFSSD+D group for offspring. The body weights of male rats from 5 to 8 weeks of age were higher in the HFSSD group than in the CD group （ all P<0.05），but not statistically significant compared with the HFSSD+D group. The total sperm count and sperm motility of male rats in both the sire and offspring HFSSD groups were lower than those in the CD and HFSSD+D groups （ both P<0.05），and the sperm malformation rate was higher than that in the CD group（ P<0.05），but not statistically significant compared with the HFSSD+D group. The conception rate and litter size of females in the paternal HFSSD group were lower than those in the CD and HFSSD+D groups（ both P<0.05）. The conception rate and litter size of females in the offspring HFSSD group were lower than those in the CD group（ both P<0.05），but not statistically significant compared with the HFSSD+D group. Compared with the CD group，the male testicular spermatogenic tubules in the sire and offspring HFSSD groups had a thinner wall and a looser and more disorganized intercellular arrangement；the male testicular spermatogenic tubules in the HFSSD+D group had more cell layers than those in the HFSSD group，and the intercellular arrangement in the sire HFSSD+D group was less disorganized than that in the offspring HFSSD+D group. Conclusion：Vitamin D intervention can improve the sperm quality of the offspring of male rats on a high-salt，high-fat，high-sugar diet，and has potential to improve offspring fertility，which has implications for eugenic reproduction.

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备注/Memo

备注/Memo:
作者简介叶舒婷（1995-），女，硕士在读，研究方向：生殖医学中的子代安全性，辅助生殖；
通信作者：王建梅，E-mail：wangjianmei@tmu.edu. cn。
更新日期/Last Update: 2023-07-10