|本期目录/Table of Contents|

[1]李秋菊,胡立娟,陈喜娟,等.高脂饮食抑制胰岛素受体和胆囊收缩素受体在小鼠肝脏中的表达[J].天津医科大学学报,2020,26(06):510-513.
　LI Qiu-ju,HU Li-juan,CHEN Xi-juan,et al.High fat diet inhibits the expression of insulin receptor and cholecystokinin receptor in mouse liver[J].Journal of Tianjin Medical University,2020,26(06):510-513.
点击复制

高脂饮食抑制胰岛素受体和胆囊收缩素受体在小鼠肝脏中的表达(PDF)
分享到：

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
26卷
期数:
2020年06期
页码:
510-513
栏目:
基础医学
出版日期:
2020-11-20

文章信息/Info

Title:
High fat diet inhibits the expression of insulin receptor and cholecystokinin receptor in mouse liver
作者:
李秋菊1; 胡立娟2; 陈喜娟1; 邱帅1; 王丰2
1.天津医科大学研究生院，天津300070；2.天津南开医院，天津市急腹症器官损伤与中西医结合修复重点实验室， 天津 300100
Author(s):
LI Qiu-ju1; HU Li-juan2; CHEN Xi-juan1; QIU Shuai1; WANG Feng2
1.Graduate School，Tianjin Medical University，Tianjin 300070，China；2.The Laboratory of Acute Abdomen Disease Associated Organ Injury and Repair，Tianjin Nankai Hospital，Tianjin 300100，China
关键词:
高脂饮食; 肥胖; 胰岛素抵抗; 胰岛素受体; 胆囊收缩素受体
Keywords:
HFD; obesity; insulin resistance; IR; CCKR
分类号:
R363.2＋1
DOI:
-
文献标志码:
A
摘要:
目的：在高脂饮食（HFD）小鼠中研究肝脏中胰岛素受体（IR）、胆囊收缩素（CCK）受体（CCKR）的表达和 胰腺中胰岛素的储量。方法:用含15%和30%脂肪的两种HFD和对照餐（5%脂肪）喂养3组小鼠（15～16只/组）12周，用 蛋白印迹法检测IR和CCKR在肝脏中的表达；用免疫组化和ELISA测定胰腺中β细胞群落和胰岛素含量；测定门静脉和外 周血CCK和胰岛素水平。结果：15%和30% HFD使肝IR、CCKR的表达水平均下降（均P<0.05）。HFD还使胰岛β细胞面积 增加（对照组：54.57±2.72；15%HFD：68.74±1.72；30%HFD：71.61±2.32；均P<0.05）。此外，30%HFD还增加胰腺 胰岛素的储量并提升门静脉血浆中胰岛素的水平。结论：HFD降低小鼠肝脏IR和CCKR的表达，增加胰腺胰岛素储量。
Abstract:
Objective: To study the hepatic expression of insulin receptor（IR） and cholecystokinin （CCK） receptor （CCKR） and pancreatic storage of insulin in high fat diet（HFD） mice. Methods: Mice in three groups（15-16 per group） were fed with two HFDs with 15% and 30% fat and a control diet（5% fat）. After 12 weeks，the expression of IR and CCKR in liver were detected by Western blotting. In the pancreas，β-cell area and insulin content were determined by immunohistochemistry and ELISA. CCK and insulin levels were measured in portal vein and peripheral blood. Results: Hepatic IR and CCKR expression was decreased by 15% and 30% HFDs （both P<0.05）. The β-cell area of the pancreas was increased by both HFDs（control diet：54.57±2.72；15%HFD: 68.74±1.72；30%HFD：71.61±2.32； all P<0.05）. Further，30% HFD increased both pancreatic insulin storage（P<0.05） and portal vein insulin. Conclusion: HFD decreases the expression of hepatic IR and CCKR and increases pancreatic insulin storage in mice.

参考文献/References:

[1] Lancet Gastroenterol Hepatol. Obesity management: gastroenterologists take centre stage[J]. Lancet Gastroenterol Hepatol，2017，2（7）：463
[2] Farmer T D，Jenkins E C，O′brien T P，et al. Comparison of the physiological relevance of systemic vs. portal insulin delivery to evaluate whole body glucose flux during an insulin clamp[J]. Am J Physiol Endocrinol Metab，2015，308（3）：E206
[3] Haeusler R A，Mcgraw T E，Accili D. Biochemical and cellular properties of insulin receptor signalling[J]. Nat Rev Mol Cell Biol，2018,19（1）：31
[4] Irwin N，Montgomery I A，Moffett R C，et al. Chemical cholecystokinin receptor activation protects against obesity-diabetes in high fat fed mice and has sustainable beneficial effects in genetic ob/ob mice[J]. Biochem Pharmacol，2013，85（1）：81
[5] Rehfeld J F. Cholecystokinin-from local gut hormone to ubiquitous messenger[J]. Front Endocrinol （Lausanne），2017,8（8）：47
[6] Rehfeld J F. Cholecystokinin expression in tumors： biogenetic and diagnostic implications[J]. Future Oncol，2016，12（18）：2135
[7] Tucker R D，Ciofoaia V，Nadella S，et al. A cholecystokinin receptor antagonist halts nonalcoholic steatohepatitis and prevents hepatocellular carcinoma[J]. Dig Dis Sci,2020,65（1）：189
[8] Okabayashi Y，Otsuki M，Baba S. Cholecystokinin in the entero-insular axis[J]. Diabetes Res Clin Pract，1989,7：S79
[9] Calanna S，Piro S，Pino A D，et al. Beta and alpha cell function in metabolically healthy but obese subjects：relationship with entero-insular axis[J]. Obesity，2013,21（2）： 320
[10] Linnemann A K，Davis D B. Glucagon-like peptide-1 and cholecystokinin production and signaling in the pancreatic islet as an adaptive response to obesity[J]. J Diabetes Investig，2016，7（Suppl 1）：44
[11] Nishikawa S，Yasoshima A，Doi K，et al. Involvement of sex，strain and age factors in high fat diet-induced obesity in C57BL/6J and BALB/cA mice[J]. Exp Anim，2007，56（4）： 263
[12] Li J L，Wu H S，Liu Y T，et al. High fat diet induced obesity model using four strains of mice： Kunming，C57BL/6，BALB/c and ICR[J].Exp Anim，2020，69（3）：326
[13] 黄金灿,张磊,常永生.KLF9基因腺病毒载体的构建及其脂肪酸氧化功能[J].天津医科大学学报，2020，26（3）： 193
[14] Kaptaner B.Immunohistochemical distribution of insulin-，glucagon- and somatostatin-containing cells in the pancreas of Lake Van fish （Alburnus tarichi Güldenst?覿dt，1814）（Cyprinidae）[J]. Eur J Histochem，2019，63（1）：2999
[15] Tierney A C，Roche H M. The potential role of olive oil-derived MUFA in insulin sensitivity[J]. Mol Nutr Food Res，2007，51（10）：1235
[16] Storlien L H，Baur L A，Kriketos A D，et al. Dietary fats and insulin action[J]. Diabetologia， 1996，39（6）：621
[17] Βoland Β Β，Rhodes C J，Grimsby J S. The dynamic plastiity of insulin production in β-cells [J]. Mol Metab，2017，6（9）：958
[18] Soli A H，Kahn C R，Neville D M，et al. Insulin receptor deficiency in genetic and acquired obesity[J]. J Clin Invest，1975，56（4）：769
[19] Zhan J，Weng J，Hunt B G，et al. Apolipoprotein A-IV enhances cholecystokinnin secretion[J]. Physiol Behav，2018,188： 11
[20] Kohan A B，Wang F，Lo C M，et al. ApoA-IV： current and emerging roles in intestinal lipid metabolism，glucose homeostasis，and satiety[J]. Am J Physiol Gastrointest Liver Physiol，2015，308（6 ）：G472
[21] Moran T H，Bi S. Hyperphagia and obesity in OLETF rats lacking CCK-1 receptors[J]. Philos Trans R Soc Lond B Biol Sci，2006， 361（1471）：1211

相似文献/References:

[1]马晓芳,童静凯,牛文彦.肥胖和缺氧对小鼠原代脂肪细胞分泌TNFα的影响[J].天津医科大学学报,2014,20(01):1.
　MA Xiao-fang,TONG Jing-kai,NIU Wen-yan.Effect of obesity and hypoxia on TNFa secretion by primary adipocytes in mice[J].Journal of Tianjin Medical University,2014,20(06):1.
[2]杨传慧,李 萍,何 庆,等.不同时间持续缺氧对3T3-L1脂肪细胞脂肪因子基因表达的影响[J].天津医科大学学报,2015,21(03):1.
　YANG Chuan-hui,LI Ping,HE Qing,et al.Effect of incubation time of hypoxia on the levels of adipokines in the 3T3-L1 adipocytes[J].Journal of Tianjin Medical University,2015,21(06):1.
[3]张秋平,夏 天,王安凤.吡格列酮对肥胖小鼠血清抵抗素的影响及其对肾脏的作用[J].天津医科大学学报,2015,21(03):223.
　ZHANG Qiu-ping,XIA Tian,WANG An-feng.Effect of pioglitazone on serum resistin and kidney in obesity mice[J].Journal of Tianjin Medical University,2015,21(06):223.
[4]杨传慧,李 萍,何 庆,等.不同时间持续缺氧对3T3-L1脂肪细胞脂肪因子基因表达的影响[J].天津医科大学学报,2015,21(01):1.
　YANG Chuan-hui,LI Ping,HE Qing,et al. Effect of incubation time of hypoxia on the expression of adipokines in the 3T3-L1 adipocytes[J].Journal of Tianjin Medical University,2015,21(06):1.
[5]赵菊兰,夏 天,李 荣,等.单纯性肥胖患者血清脂联素、血清瘦素与肾损伤的相关性分析[J].天津医科大学学报,2016,22(01):34.
　ZHAO Ju-lan,XIA Tian,LI Rong,et al.Correlation analysis of serum adiponectin and serum leptin to renal damage in patients with simply obesity[J].Journal of Tianjin Medical University,2016,22(06):34.
[6]吴 璠,华绍芳,李月琴.孕前肥胖对早发型子痫前期围产结局及新生儿血气的影响[J].天津医科大学学报,2016,22(05):442.
[7]张诗田,张 达,牛文彦.肥胖患者炎性单核细胞Caspase活性的研究[J].天津医科大学学报,2017,23(02):138.
　ZHANG Shi-tian,ZHANG Da,NIU Wen-yan.Caspase activity of pro-inflammatory monocytes in obese patients[J].Journal of Tianjin Medical University,2017,23(06):138.
[8]彭泽椿,郑向前,高 明.甲状腺乳头状癌临床病理学特征与肥胖的相关性研究[J].天津医科大学学报,2018,24(05):429.
　PENG Ze-chun,ZHENG Xiang-qian,GAO Ming.Correlation between body mass index and clinicopathological characteristics of papillary thyroid carcinoma[J].Journal of Tianjin Medical University,2018,24(06):429.
[9]叶舒婷,杜湧瑞,王建梅.维生素D干预对高盐高脂高糖饮食雄性小鼠的子代生育能力的影响[J].天津医科大学学报,2023,29(04):413.
　YE Shu-ting,DU Yong-rui,WANG Jian-mei.Effect of vitamin D intervention on offspring fertility in male mice on a high-salt，high-fat，high-sugar diet[J].Journal of Tianjin Medical University,2023,29(06):413.
[10]任惠珠,马泽军,王珊珊,等.Frohlich综合征伴2型糖尿病1例[J].天津医科大学学报,2023,29(06):666.

备注/Memo

备注/Memo:
文章编号 1006-8147（2020）06-0510-04
基金项目 国家自然科学基金（81572318）
作者简介 李秋菊（1993-），女，硕士在读，研究方向：代谢和癌症；通信作者：王丰，E-mail: fengwangpi@163.com。
更新日期/Last Update: 2020-11-20