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[1]王亚霓,刘欣,张颖,等.牛磺酸对大鼠缺血心肌血管新生作用的研究[J].天津医科大学学报,2014,20(01):4-007.
　WANG Ya-ni,LIU Xin,et al. Effects of taurine on ischemic/reperfusion myocardium angiogenesis in rats[J].Journal of Tianjin Medical University,2014,20(01):4-007.
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牛磺酸对大鼠缺血心肌血管新生作用的研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20卷
期数:
2014年01期
页码:
4-007
栏目:
基础医学
出版日期:
2014-01-20

文章信息/Info

Title:

Effects of taurine on ischemic/reperfusion myocardium angiogenesis in rats

文章编号:
1006-8147(2014)01-0004-04
作者:
王亚霓1; 刘欣1; 张颖2; 张曦2; 王意涵3; 徐哲龙1
(1.天津医科大学病理生理学教研室,天津 300070;2. 天津医科大学药理学教研室,天津 300070;3.天津医科大学第二医院泌尿外科,天津 300211)
Author(s):

WANG Ya-ni1; LIU Xin1; ZHANG Ying2; ZHANG Xi2; WANG Yi-han3; XU Zhe-long1

(1.Department of Pathophysiology，Tianjin Medical University,Tianjin 300070,China;2.Department of Pharmacology,Tianjin Medical University, Tianjin 300070,China;?

3. Department of Urology ,the Second Hospital，Tianjin Medical University,Tianjin 300211, China)

关键词:

牛磺酸; 心肌新生血管; 缺血/再灌注; 血小板-内皮细胞粘附分子; 血管内皮生长因子; 大鼠

Keywords:
taurine;  angiogenesis;  ischemia-reperfusion;  CD34;  VEGF;  rat
分类号:
R363.1+3
DOI:
-
文献标志码:
A
摘要:

目的 : 研究牛磺酸对大鼠缺血心肌血管新生的作用并探讨其作用机制。方法：选用健康雄性 Wistar 大鼠 80 只，随机分为 4 组，每组 20 只：缺血再灌注模型组，低、中、高剂量牛磺酸处理组。缺血再灌注模型组口服生理盐水 10 mL/kg, 每日 1 次，连续 7 d 。低、中、高剂量牛磺酸处理组牛磺酸 200 mg/kg 、 400 mg/kg 、 800 mg/kg 每日 1 次，口服给药，连续 7 d 。采用大鼠心脏冠状动脉缺血再灌注的方法，连续监测心电图，观察牛磺酸对大鼠心肌缺血再灌注后心律失常、心肌梗死范围的影响，并观察牛磺酸对血清中血管内皮生长因子（ VEGF ）、低氧诱导因子 - α （ HIF-α ） 、血小板 - 内皮细胞粘附分子（ CD34 ）以及心肌组织中 VEGF 含量的影响。结果 : 与对照组比较，随着剂量的增高 可明显降低动物室性早搏（ VPC ）和室性心动过速（ VT ）的发生率， 心肌梗死范围也显著减小，同时在光镜下观察各组随牛磺酸剂量的递增可促进心肌间的血管新生。牛磺酸高、中、低剂量组大鼠血清中 VEGF 、 HIF-α 、 CD34 与心肌中的 VEGF 活性明显升高（ P <0.01 ）。结论：牛磺酸对大鼠缺血心肌血管新生具有促进作用。

Abstract:
Objective: To study the effects of taurine on ischemic/reperfusion myocardium angiogenesis in rats. Methods: Eighty healthy male Wistar rats were randomly divided into four groups, with n=20 in each group: The ischemia-reperfusion group and the taurine of 3 dosages groups. The ischemia-reperfusion group was directly given normal saline 10ml/kg with oral administration once a day for one week. Each of taurine group was given oral administration 200 mg/kg, 400 mg/kg,800 mg/kg, respectively, once a day for one week. The model of myocardial ischemia-reperfusion injury was established in all of the groups. Blood pressure and ECG were obtained. Myocardial infarct size was examined. Activities of VEGF, HIF-α and CD34 were detected. Results: Compared with the control group, taurine significantly decreased the incidence of ventricular premature contraction (VPC) and ventricular tachycardia (VT) in rats during ischemia，and the myocardial infarction was alleviated in each taurine group in dosage-dependent manner. The levels of VEGF, HIF-α, CD34 and myocardium level of VEGF significantly increased, and infarct size of myocardial was significantly reduced. Conclusion: The myocardial angiogenesis may be involved in the mechanism of taurine in protecting myocardium against ischemic reperfusion injury

参考文献/References:

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（81200534）
作者简介 王亚霓（1989-），女，硕士在读，研究方向：心血管病理生理学；通信作者：刘欣，E-mail:liuxin@tijmu.edu.cn。
更新日期/Last Update: 2014-03-24