|本期目录/Table of Contents|

[1]丁子特,韩继媛,倪春生,等.LMNB1表达对乳腺癌细胞增殖的影响及其临床意义[J].天津医科大学学报,2025,31(04):295-299.[doi:10.20135/j.issn.1006-8147.2025.04.0295]
　DING Zite,HAN Jiyuan,NI Chunsheng,et al.Effect of LMNB1 expression on the proliferation of breast cancer cells and its clinical significance[J].Journal of Tianjin Medical University,2025,31(04):295-299.[doi:10.20135/j.issn.1006-8147.2025.04.0295]
点击复制

LMNB1表达对乳腺癌细胞增殖的影响及其临床意义(PDF)

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
31卷
期数:
2025年04期
页码:
295-299
栏目:
肿瘤疾病专题
出版日期:
2025-07-10

文章信息/Info

Title:
Effect of LMNB1 expression on the proliferation of breast cancer cells and its clinical significance
文章编号:
1006-8147(2025)04-0295-06
作者:
丁子特; 韩继媛; 倪春生; 刘芳; 胡飞云; 王丹; 董学易; 张丹芳
（天津医科大学病理学教研室，天津 300070；天津医科大学总医院病理科，天津 300052）
Author(s):
DING Zite;  HAN Jiyuan;  NI Chunsheng;  LIU Fang;  HU Feiyun;  WANG Dan;  DONG Xueyi;  ZHANG Danfang
（Department of Pathology， Tianjin Medical University， Tianjin 300070， China； Department of Pathology， General Hospital of Tianjin Medical University， Tianjin 300052， China）
关键词:
LMNB1; 乳腺癌; 增殖; 治疗靶点
Keywords:
LMNB1;  breast cancer;  proliferation;  targets for treatment
分类号:
R737.9
DOI:
10.20135/j.issn.1006-8147.2025.04.0295
文献标志码:
A
摘要:
目的：研究核纤层蛋白B1 （LMNB1）的表达对乳腺癌细胞增殖的影响及其临床意义。方法：通过The Human Protein Atlas 数据库中免疫组织化学染色明确LMNB1在乳腺癌组织中的表达部位。利用Breast Cancer Integrative Platform和UALCAN临床数据库分析LMNB1在乳腺癌组织中的表达及与临床病理参数以及乳腺癌患者预后的关系。选取乳腺癌细胞MCF-7，将其分为对照组及LMNB1过表达组，实时荧光定量PCR验证过表达效果，通过CCK-8和MTT实验检测其对乳腺癌细胞增殖能力的影响，通过伤口愈合实验检测其对乳腺癌细胞迁移能力的影响。结果：数据库分析结果显示， LMNB1主要在乳腺癌组织的核膜中表达。其在亚洲人群中表达水平较高；在21～40岁患者中最高（均P＜0.001）；在N2期淋巴结转移患者中显著高于N3期及正常组织（均P＜0.01）；在晚期患者中更为显著（均P＜0.05）；在三阴性乳腺癌患者及TP53突变的乳腺癌中表达更高（均P＜0.05）。总生存期（P＜0.01，HR=1.348）、疾病特异性生存期（P＜0.001，HR=1.875）、无病生存期（P＜0.001，HR=4.696）、无复发生存期（P＜0.001，HR=4.126）、无远处转移生存期（P＜0.01，HR=2.723）与患者较差的预后相关。实验结果显示，与正常乳腺组织相比，LMNB1在乳腺癌组织中的表达水平升高（P<0.000 1）。CCK-8和MTT实验结果显示，与对照组相比，高表达LMNB1增强了乳腺癌细胞的增殖能力（t=4.007、11.09，均P＜0.05）；伤口愈合实验结果也表明，与对照组相比，LMNB1高表达促进乳腺癌细胞的迁移能力。结论：LMNB1能够促进细胞增殖、迁移，可作为乳腺癌预后的生物标志物及治疗靶点。
Abstract:
Objective：To study the effect of lamin B1（LMNB1） expression on the proliferation of breast cancer cells and its clinical significance. Methods： The expression site of LMNB1 in breast cancer tissues was determined by immunohistochemistry staining in The Human Protein Atlas database. The Breast Cancer Integrative Platform and the UALCAN clinical database were utilized to examine LMNB1 expression in breast cancer tissues and its associations with clinicopathological parameters and prognosis of breast cancer patient. Breast cancer cells MCF-7 were selected and divided into the control group and the LMNB1 overexpression group. The overexpression effect was verified by real-time fluorescence quantitative PCR. The effects of MCF-7 on the proliferation ability of breast cancer cells was detected by CCK-8 and MTT experiments， and its influence on the migration ability of breast cancer cells was detected by wound healing experiments. Results： The results of database analysis showed that LMNB1 was mainly expressed in the nuclear membrane of breast cancer tissues. Its expression level was relatively high among the Asian population. It was the highest among patients aged 21 to 40 （all P<0.001）. It was significantly higher in patients with stage N2 lymph node metastasis than in stage N3 and normal tissues（all P<0.05）. It was more significant among patients in the advanced stage（all P<0.05）. The expression was higher in patients with triple-negative breast cancer patients and breast cancer patients with TP53 mutations（all P<0.05）. The overall survival period （P<0.01， HR=1.348）， disease-specific survival period （P<0.001，HR=1.875）， disease-free survival period （P<0.001， HR=4.696）， recurrence-free survival period （P<0.001， HR=4.126）， and distant metastasis-free survival period （P<0.01， HR=2.723）were associated with the poor prognosis of patients. The experimental results showed that， compared with normal breast tissue， the expression level of LMNB1 in breast cancer was increased（P<0.000 1）. Meanwhile， the results of CCK-8 and MTT experiments showed that， compared with the control group， high expression of LMNB1 enhanced the proliferation ability of breast cancer cells （t=4.007， 11.09， both P<0.05）. The results of the wound healing experiment also indicated that， compared with the control group， the high expression of LMNB1 promoted the migration ability of breast cancer cells. Conclusion： LMNB1 can promote the proliferation and migration of breast cancer cells， and can be used as a prognostic biomarker and therapeutic target for breast cancer.

参考文献/References:

[1] HICKEY M， BASU P， SASSARINI J， et al. Managing menopause after cancer[J]. Lancet， 2024， 403（10430）： 984-996.
[2] MOU J，LI C， ZHENG Q， et al. Research progress in tumor angiogenesis and drug resistance in breast cancer [J]. Cancer Biol Med， 2024， 21（7）： 571-585.
[3] CAI Y， LIU Y， SUN Y， et al. Mesenchymehomeobox 2 inhibits ca-ncer in breast carcinoma by affecting the PI3K/AKT/mTOR and ERK1/2 pathways[J]. Biochem Biophys Res Commun， 2022， 593： 20-27.
[4] PANKOTAI-BOD?譫 G， OL?譧H-N?譩METH O， S?譈K?魻SD F， et al. Routine molecular applications and recent advances in breast cancer diagnostics[J]. J Biotechnol， 2024， 380： 20-28.
[5] CREMER T， CEMER M. Chromosometerritories[J]. Cold Spring Harb Perspect Biol， 2010， 2（3）： a003889.
[6] DECHAT T， PFLEGHAAR K， SENGUPTA K， et al. Nuclearla-mins： major factors in the structural organization and function of the nucleus and chromatin[J]. Genes Dev， 2008， 22（7）： 832-853.
[7] ZHAO C L， YU S， WANG S H， et al. Characterization of cluster of differentiation 47 expression and its potential as a therapeutic target in esophageal squamous cell cancer[J]. Oncol Lett， 2018， 15（2）： 2017-2023.
[8] GIGANTE， C M， DIBATTISTA M， et al. Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation[J]. Nat Commun， 2017， 8： 15098.
[9] PADIATH Q S， SAIGOH K， SCHIFFMANN R， et al. Lamin B1 duplications cause autosomal dominant leukodystrophy[J]. Nat Ge-net， 2006， 38（10）： 1114-1123.
[10] HUA Y， HE Z， ZHANG X. A pan-canceranalysis based on weighted gene co-expression network analysis identifies the biomarker utility of lamin B1 in human tumors[J]. Cancer Biomarkers， 2022， 34（1）： 23-39.
[11] VASHISTH M， CHO S， IRIANTO J， et al. Scaling concepts in ′omics： Nuclear lamin-B scales with tumor growth and often predicts poor prognosis， unlike fibrosis[J]. Proc Natl Acad Sci U S A， 2021，118（48）： e2112940118.
[12] HOU X， OUYANG J， TANG L， et al.KCNK1 promotes proliferation and metastasis of breast cancer cells by activating lactate dehydrogenase A （LDHA） and up-regulating H3K18 lactylation[J]. PLoS Biol， 2024， 22（6）： e3002666.
[13] XIA C， DONG X， LI H， et al. Cancer statistics in China and United States， 2022： profiles， trends， and determinants[J]. Chin Med J （Engl）， 2022， 135（5）： 584-590.
[14] SUNG H， FERLAY J， SIEGEL R L， et al. Global Cancer Statistics 2020： GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries[J]. CA Cancer J Clin， 2021， 71（3）： 209-249.
[15] MAOMAO C， HE L， DIANQIN S，et al. Current cancer burden in China： epidemiology， etiology， and prevention[J]. Cancer Biol Med， 2022， 19（8）： 1121-1138.
[16] YANG Y， XIAO W， LIU R， et al. A lamin family-based signature predicts prognosis and immunotherapy response in hepatocellular carcinoma[J]. J Immunol Res， 2022， 2022：4983532.
[17] TANG D， LUO H， XIE A， et al. Silencing LMNB1 contributes to the suppression of lung adenocarcinoma development[J]. Cancer Manag Res， 2021， 13： 2633-2642.
[18] YANG Y， GAO L， CHEN J， et al. Lamin B1 is a potential therapeutic target and prognostic biomarker for hepatocellular carcinoma[J]. Bioengineered， 2022， 13（4）： 9211-9231.
[19] L?魧MMERHIRT L， KAPPELMANN-FENZL M， FISCHER S， et al.Knockdown of lamin B1 and the corresponding lamin B receptor leads to changes in heterochromatin state and senescence induction in malignant melanoma[J]. Cells， 2022， 11（14）： 2154.
[20] WEISZ A， ABADI U， MAUSBACH L， et al. Nuclear αvβ3 integrin expression， post translational modifications and regulation in hematological malignancies[J]. Hematol Oncol， 2022， 40（1）： 72-81.

相似文献/References:

[1]朱悦,张诗武,张丹芳,等.TA2小鼠自发乳腺癌血清蛋白质组学研究[J].天津医科大学学报,2013,19(05):373.
[2]刘 营,孙保存,刘铁菊,等.AURKA蛋白激酶在三阴乳腺癌干细胞形成血管拟态中的实验研究[J].天津医科大学学报,2013,19(06):437.
　LIU Ying,SUN Bao-cun,LIU Tie-ju,et al.Experimental study of AURKA protein kinase in the formation of vascular mimicry in triple-negative breast cancer stem cells[J].Journal of Tianjin Medical University,2013,19(04):437.
[3]伦淑敏.HOXA5基因真核表达质粒的构建及在乳腺癌细胞中的功能研究[J].天津医科大学学报,2014,20(05):337.
　LUN Shu-min. Construction of HOXA5 eukaryotic expression plasmid of and its biological significance in breast cancer cells[J].Journal of Tianjin Medical University,2014,20(04):337.
[4]伦淑敏.肌细胞增强因子2A基因真核表达质粒的构建及对乳腺癌细胞MCF-7增殖能力的影响[J].天津医科大学学报,2014,20(06):429.
　LUN Shu-min.Construction of myocyte enhancer factor 2A eukaryotic expression plasmid and effects on cell proliferation in breast cancer cell line MCF7[J].Journal of Tianjin Medical University,2014,20(04):429.
[5]孙秀梅,张 飞,田 然,等.Nanog表达上调促进乳腺癌细胞MCF-7的增殖和侵袭[J].天津医科大学学报,2014,20(06):421.
　SUN Xiu-mei,ZHANG Fei,TIAN Ran,et al.Up-regulation of Nanog promotes cell proliferation and invasion in breast cancer cells MCF-7[J].Journal of Tianjin Medical University,2014,20(04):421.
[6]张 洁,张 飞,冀 为,等. SHP2不同突变体对乳腺癌细胞的迁移和侵袭能力的影响[J].天津医科大学学报,2015,21(02):93.
　ZHANG Jie,ZHANG Fei,JI Wei,et al. Effect of different SHP2 mutants on breast cancer cell migration and invasion[J].Journal of Tianjin Medical University,2015,21(04):93.
[7]蔡 隽. FOXQ1稳定表达乳腺癌细胞系的建立及鉴定[J].天津医科大学学报,2015,21(04):292.
　CAI Jun.Establishment and identification of cell lines with stable expression of FOXQ1 in MDA-MB-231-luc[J].Journal of Tianjin Medical University,2015,21(04):292.
[8]蔡 隽 综述,冯玉梅 审校.叉头框转录因子调控乳腺癌生物学特性的研究进展[J].天津医科大学学报,2015,21(05):455.
[9]任宗娜.沉默Notch4基因对乳腺癌细胞系MDA-MB-231增殖和迁移侵袭能力的影响[J].天津医科大学学报,2015,21(06):469.
　REN Zong-na.Inhibition effect of?silencing?? Notch4 gene on the proliferation and migration and invasion activity of? breast cancer cell line?MDA-MB-231[J].Journal of Tianjin Medical University,2015,21(04):469.
[10]周岩,宋伟杰,张飞,等.人附睾蛋白4在乳腺癌发生发展中的机制研究[J].天津医科大学学报,2015,21(06):466.
　ZHOU Yan,SONG Wei-jie,ZHANG Fei,et al.Mechanism of human epididymis protein 4 in development and progression of breast cancer[J].Journal of Tianjin Medical University,2015,21(04):466.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金（82373278）；天津医科大学基础医学院青年教师科研孵育基金（2023FY08）
作者简介:丁子特（1998-），女，硕士在读，研究方向：病理学；通信作者：张丹芳，E-mail： zhangdf@tmu.edu.cn。
更新日期/Last Update: 2025-07-10