|本期目录/Table of Contents|

[1]刘 营,孙保存,刘铁菊,等.AURKA蛋白激酶在三阴乳腺癌干细胞形成血管拟态中的实验研究[J].天津医科大学学报,2013,19(06):437-440.
 LIU Ying,SUN Bao-cun,LIU Tie-ju,et al.Experimental study of AURKA protein kinase in the formation of vascular mimicry in triple-negative breast cancer stem cells[J].Journal of Tianjin Medical University,2013,19(06):437-440.
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AURKA蛋白激酶在三阴乳腺癌干细胞形成血管拟态中的实验研究
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
19
期数:
2013年06期
页码:
437-440
栏目:
基础医学
出版日期:
2013-11-20

文章信息/Info

Title:
Experimental study of AURKA protein kinase in the formation of vascular mimicry in triple-negative breast cancer stem cells
作者:
刘 营1孙保存 12刘铁菊1赵秀兰1李岩磊1
1.天津医科大学病理学教研室,天津300070;2.天津医科大学附属肿瘤医院病理科,天津300060
Author(s):
LIU Ying1 SUN Bao-cun1 2 LIU Tie-ju1 ZHAO Xiu-lan1 LI Yan-lei1
1.Department of Pathology, Tianjin Medical University, Tianjin 300070, China; 2.Department of Pathology, Cancer Hospital, Tianjin Medical University, Tianjin 300060, China
关键词:
AURKA乳腺癌干细胞血管拟态
Keywords:
-
分类号:
R737.9
DOI:
-
文献标志码:
A
摘要:
目的:探讨AURKA蛋白激酶在三阴乳腺癌干细胞形成血管拟态中的作用。方法:用无血清悬浮培养法从三阴乳腺癌细胞MDA-MB-231分离获得干细胞,RT-PCR检测干细胞转录因子c-myc、sox-2的表达状态;三维培养观察干细胞形成血管能力;Western blot 检测AURKA蛋白激酶在干细胞和常规培养细胞的表达;利用干细胞进行三维培养并加入AURKA蛋白激酶抑制剂观察对成血管能力的影响。结果:(1)利用干细胞无血清悬浮培养可获得MDA-MB-231干细胞,其干细胞转录因子c-myc、sox-2表达高于常规培养细胞。(2)AURKA蛋白激酶在乳腺癌干细胞球中较常规培养细胞表达升高。(3)干细胞三维培养可形成血管管道,加入AURKA蛋白激酶抑制剂后成血管能力减弱。结论:AURKA蛋白激酶可促进三阴乳腺癌干细胞形成血管拟态,抑制其表达能减弱形成血管拟态能力。
Abstract:
Objective: To investigate the role of AURKA protein kinase in the formation of vascular mimicry in triple-negative breast cancer stem cells. Methods: The serum-free suspension culture of breast spheres from triple-negative breast cancer cells MDA-MB-231 cells were isolated, the expression of stem cell transcription factor status of c-myc and sox-2 were detected by RT-PCR. The formation of vascular stem cells were observed by three-dimensional culture. The expression of AURKA protein kinase from stem and normal cells were detected by western blot. Three-dimensional culture and AURKA protein kinase inhibitor were used to detect the formation ability of blood vessels. Results: (1) The expression of stem cell transcription factor status of c-myc and sox-2 were highter than conventional cell culture in MDA-MB-231 stem cells obtained from the serum-free suspension culture. (2) The expression of AURKA protein kinase were higher than conventional cell culture in MDA-MB-231 stem cells obtained from the serum-free suspension culture.(3) Stem cells contributed to the formation of vascular pipeline, AURKA protein kinase inhibitor diminished the formation of vascular. Conclusion: AURKA plays a key role in the formation of vascular mimicry in triple-negative breast cancer stem cells,inhibiting its expression and weakening the ability of the formation of vascular mimicry.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金重点项目(81230050);天津市科委项目(125JCYBJC15500)作者简介 刘营(1986-),男,硕士在读,研究方向:肿瘤血管生成;通信作者:孙保存,E-mail:sunbaocun@aliyun.com。
更新日期/Last Update: 2013-12-20