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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

27卷

期数:

2021年05期

页码:

472-476

栏目:

基础医学

出版日期:

2021-09-10

文章信息/Info

Title:

Effects of miR-133 overexpression on apoptosis，proliferation and tumor formation of colon cancer cells SW480 in nude mice

文章编号:

1006-8147(2021)05-0472-05

作者:

潘琼; 谷见法; 刘松格; 杨晓瑞; 易善永

（郑州市中心医院肿瘤内科，郑州 ⁴⁵0000）

Author(s):

PAN Qiong; GU Jian-fa; LIU Song-Ge; YANG Xiao-rui; YI Shan-yong

（Department of Oncology，Zhengzhou Central Hospital，Zhengzhou，450000）

关键词:

结肠癌; miR-133; 凋亡; 增殖

Keywords:

colon cancer;  miR-133;  apoptosis;  proliferation

分类号:

R735.3+5

DOI:

-

文献标志码:

A

摘要:

目的：探究miR-133过表达对结肠癌细胞SW480凋亡、增殖和裸鼠成瘤的影响。方法：将miR-133 mimic转染至SW480细胞，将细胞分为3组：对照组、Scramble组和miR-133 mimic组。RT-PCR检测miR-133表达水平；流式细胞仪检测细胞凋亡；Western 印迹检测Bcl-2、Bax、Caspase-3、Caspase-9、c-Myc蛋白表达水平；CCK-8法检测细胞增殖倍数；克隆形成实验检测细胞克隆形成率；裸鼠右侧腋窝处皮下注射SW480细胞悬液建立裸鼠移植瘤模型，检测肿瘤重量，RT-PCR检测肿瘤组织miR-133表达水平，免疫组化检测Caspase-3、Ki67阳性细胞数。结果：与对照组相比，miR-133 mimic组miR-133表达升高（F=136.70，P＜0.05），细胞凋亡率升高（F=59.995，P＜0.05），Bax/Bcl-2、cleaved Caspase-3/Caspase-3、cleaved Caspase-9/Caspase-9表达升高（F=726.85、138.76、55.85，均P＜0.05），c-Myc蛋白表达水平降低（F=88.98，P＜0.05），细胞活力降低（F=48.50，P＜0.05），克隆形成率降低（F=42.63，P＜0.05），肿瘤重量降低（t=1.788，P＜0.05），肿瘤组织miR-133表达水平升高（t=1.043，P＜0.05），Caspase-3阳性细胞数升高（t=1.167，P＜0.05），Ki67阳性细胞数降低（t=1.557，P＜0.05）。结论：miR-133过表达诱导SW480细胞凋亡，抑制SW480细胞增殖和裸鼠肿瘤的形成。

Abstract:

Objective：To investigate the effects of miR-133 overexpression on the apoptosis，proliferation and tumor formation of colon cancer cells SW480 in nude mice. Methods：SW480 cells were transfected with miR-133 mimic. The cells were divided into three groups：control group，Scramble group and miR-133 mimic group. The expression of miR-133 was detected by RT-PCR. Flow cytometry was used to detect apoptosis. Western blotting was used to detect the expression of Bcl-2，Bax，Caspase-3，Caspase-9 and c-Myc. Cell proliferation was measured by CCK-8 method. The clone formation rate of cells was detected by clone formation experiment. The xenograft model of nude mice was established by subcutaneous injection of SW480 cell suspension in the right armpit，tumor weight was detected，the expression of miR-133 in tumor tissues was detected by RT-PCR，and the number of Caspase-3 and Ki67 positive cells were detected by immunohistochemistry. Results：Compared with control group，miR-133 mimic group showed increased expression of miR-133（F=136.70，P<0.05），increased apoptosis rate（F=59.995，P<0.05），increased Bax/Bcl-2，cleaved Caspase-3/Caspase-3，and cleaved Caspase-9/Caspase-9 expression（F=726.85，138.76，55.85，all P<0.05），and cell apoptosis rate（F=59.995，P<0.05），decreased C-Myc protein expression levels（F=88.98，P<0.05），decreased cell viability（F=48.50，P<0.05），decreased clone formation rate（F=42.63，P<0.05），reduced tumor weight （t=1.788，P<0.05），increased tumor tissue expression of miR-133 levels（t=1.043，P<0.05），increased Caspase-3 positive cells（t=1.167，P<0.05），reduced number of Ki67 positive cells（t=1.557，P<0.05）. Conclusion：Overexpression of miR-133 induces apoptosis of SW480 cells and inhibits SW480 cells proliferation and tumor formation in nude mice.

参考文献/References:

[1] GOMES S E，SIM?簟ES A E S，PEREIRA D M，et al. Mir-143 or mir-145 overexpression increases cetuximab-mediated antibody-dependent cellular cytotoxicity in human colon cancer cells[J]. Oncotarget，2016，7（8）：9368
[2] QI L，DING Y. Screening of differentiation-specific molecular biomarkers for colon cancer[J]. Cell Physiol Biochem，2018，46（6）：2543
[3] ZENG M，ZHU L，LI L，et al. Mir-378 suppresses the proliferation，migration and invasion of colon cancer cells by inhibiting sdad1[J]. Cell Mol Biol Lett，2017，22：12
[4] VEL?ZQUEZ K T，Enos R T，McClellan J L，et al. Microrna-155 deletion promotes tumorigenesis in the azoxymethane-dextran sulfate sodium model of colon cancer[J]. Am J Physiol Gastrointest Liver Physiol，2016，310（6）：G347
[5] ZHOU Y，WU D，TAO J，et al. Microrna-133 inhibits cell proliferation，migration and invasion by targeting epidermal growth factor receptor and its downstream effector proteins in bladder cancer[J]. Scand J Urol，2013，47（5）：423
[6] LIU S，CHEN J，ZHANG T，et al. Microrna-133 inhibits the growth and metastasis of the human lung cancer cells by targeting epidermal growth factor receptor[J]. J BUON，2019，24（3）：929
[7] CHEN X B，LI W，CHU A X. Micro RNA-133a inhibits gastric cancer cells growth，migration，and epithelial-mesenchymal transition process by targeting presenilin 1[J]. J Cell Biochem，2019，120（1）：470
[8] CHENG Z，LIU F，WANG G，et al. Mir-133 is a key negative regulator of cdc42-pak pathway in gastric cancer[J]. Cell Signal，2014，26（12）：2667
[9] MODI S，KIR D，BANERJEE S，et al. Control of apoptosis in treatment and biology of pancreatic cancer[J]. J Cell Biochem，2016，117（2）：279
[10] SIQUEIRA EC，SOUZA FT，Diniz MG，et al. Hsp27（hspb1） differential expression in normal salivary glands and pleomorphic adenomas and association with an increased bcl2/bax ratio[J]. Tumour Biol，2015，36（1）：213
[11] YANG S，LI X，DOU H，et al. Sesamin induces A549 cell mitophagy and mitochondrial apoptosis via a reactive oxygen species-mediated reduction in mitochondrial membrane potential[J]. Korean J Physiol Pharmacol，2020，24（3）：223
[12] MORISHIMA N，NAKANISHI K，TAKENOUCHI H，et al. An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12[J]. J Biol Chem，2002，277（37）：34287
[13] 孙光源，武亮，张月，等. 结肠癌组织中wwp1、c-myc的表达及临床意义[J]. 中国现代医学杂志，2019，29（10）：21
[14] ZHANG G，WANG J，ZHENG R，et al. Mir-133 targets yes1 and inhibits the growth of triple-negative breast cancer cells[J].Technol Cancer Res Treat，2020，19：1533033820927011
[15] 许荣华，何冬雷，孟冿. Mir-133在胃癌组织中的表达及对胃癌细胞恶性行为的影响[J]. 临床肿瘤学杂志，2015，7：588
[16] NAGAPPAN A，LEE W S，YUN J W，et al. Tetraarsenic hexoxide induces g2/m arrest，apoptosis，and autophagy via PI3k/Akt suppression and p38 mapk activation in sw620 human colon cancer cells[J]. PLoS One，2017，12（3）：e0174591
[17] 王东华，贾喜花，郑淑君，等. 结肠癌组织中capn4和ki-67的表达及临床意义[J]. 临床肿瘤学杂志，2017，22（3）：223
[18] 彭玉平，蒋红钢，陈治横，等. Mir-133靶向jak2抑制胃癌细胞增殖、迁移和侵袭[J]. 世界华人消化杂志，2018，26（35）：2036
[19] 胡东辉，黄橘村，张建军. Mir-133靶向表皮生长因子受体抑制肝癌细胞的侵袭和迁移[J]. 东南大学学报（医学版），2019，38（6）：1044

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备注/Memo

备注/Memo:

作者简介 潘琼（1987-），女，主治医师，硕士，研究方向：食管癌、肺癌、胃癌、结直肠癌；通信作者：易善永，E-amil：yisy2001@126.com。

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更新日期/Last Update: 2021-09-01