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[1]王小东,马博昭,戚 峰.MiR-148a-3p通过靶向SRPK2抑制结肠癌细胞转移[J].天津医科大学学报,2019,25(02):99-104109.
 WANG Xiao-dong,MA Bo-zhao,QI Feng.MiR-148a-3p inhibits colon cancer cell metastasis by targeting SRPK2[J].Journal of Tianjin Medical University,2019,25(02):99-104109.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25
期数:
2019年02期
页码:
99-104109
栏目:
基础医学
出版日期:
2019-03-20

文章信息/Info

Title:
MiR-148a-3p inhibits colon cancer cell metastasis by targeting SRPK2
文章编号:
1006-8147(2019)02-0099-06
作者:
王小东马博昭戚 峰
(天津医科大学总医院普通外科,天津 300052)
Author(s):
WANG Xiao-dong MA Bo-zhao QI Feng
(Department of General Surgery, General Hospital , Tianjin Medical University ,Tianjin 300052,China)
关键词:
miRNA结肠癌转移上皮-间质转化
Keywords:
miRNA colon cancer metastasis epithelial-mesenchymaltransition
分类号:
R735.3+5
DOI:
-
文献标志码:
A
摘要:
目的:MicroRNA (miRNA/miR)参与结肠癌各种生物学过程。目前,miR-148a-3p在结肠癌中的作用还不完全清楚。本研究旨在探讨miR-148a-3p对结肠癌细胞转移及侵袭能力的影响及机制。方法:实时定量PCR (qRT-PCR)检测结肠癌细胞系中miR-148a-3p及SRPK2 mRNA的表达,Western blot检测SRPK2 蛋白的表达。使用miR-148a-3p mimic, miR-148a-3p inhibitor调节HCT-116及SW480细胞中miR-148a-3p的水平。通过划痕及transwell实验检测miR-148a-3p对结肠癌细胞迁移及侵袭能力的影响。生物信息学分析预测miR-148a-3p的靶点,荧光素酶报告实验验证。Western blot及qRT-PCR检测miR-148a-3p对结肠癌细胞上皮-间质转化及SRPK2表达的影响。结果:与正常结直肠粘膜细胞FHC相比,结肠癌细胞系中miR-148a-3p水平降低(P<0.05)。结肠癌细胞中miR-148a-3p过表达可以明显抑制结肠癌细胞转移及侵袭能力(P<0.05)。相反的,敲低miR-148a-3p结肠癌细胞转移及侵袭能力增强(P<0.05)。荧光素酶报告系统结果提示SRPK2是miR-148a-3p的直接作用靶点。过表达miR-148a-3p可以抑制SRPK2在结肠癌中的表达(P<0.05),但是敲低miR-148a-3p时SRPK2表达明显增高(P<0.05)。结论:MiR-148a-3p可能通过靶向SRPK2抑制结肠癌细胞的转移及侵袭能力。MiR-148a-3p可能成为诊断和治疗结肠癌的靶点之一。
Abstract:
Objective: To investigate the effects of miR-148a-3p on colon cancer cell metastasis and invasion. Methods: The expressions of miR-148a-3p and SRPK2 mRNA in colon cancer cell lines were detected by quantitative real-time PCR (qRT-PCR). The expression of SRPK2 protein was detected by Western blot. MiR-148a-3p mimic, miR-148a-3p inhibitor was used to regulate the level of miR-148a-3p in HCT-116 and SW480 cells. The effects of miR-148a-3p on the migration and invasion of colon cancer cells were examined by wound-healing and transwellassay. The target of miR-148a-3p was predicted by bioinformatics analysis and was verified by luciferase reporter assay. The effects of miR-148a-3p on epithelial-mesenchymal transition and SRPK2 expression in colon cancer cells were detected by Western blot and qRT-PCR. Results: MiR-148a-3p levels were reduced in CRC cell lines as compared to normal colon cell FHC (P<0.05). Overexpression of miR-148a-3p in colon cancer cells significantly inhibited the metastasis and invasion of colon cancer cells (P<0.05). In contrast, knockdown of miR-148a-3p showed an increased metastasis and invasion of colon cancer cells (P<0.05). SRPK2 is a direct target of miR-148a-3p. Overexpression of miR-148a-3p inhibited the expression of SRPK2 in colon cancer (P<0.05), while the expression of SRPK2 was significantly increased in miR-148a-3p CRC cells (P<0.05). Conclusion: MiR-148a-3p can inhibit the metastasis and invasion of colon cancer cells by targeting SRPK2. MiR-148a-3pmight be one of the targets for the diagnosis and treatment of colon cancer.

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目(81570375) 作者简介 王小东(1990-),男,博士在读,研究方向:普通外科;通信作者:戚峰,E-mail:qf@medmail.com.cn,qifengtmu2017@163.com。
更新日期/Last Update: 2019-04-25