|本期目录/Table of Contents|

[1]王金淼,姜 涛,戚 峰,等.抑制microRNA-21表达降低HCT-116细胞侵袭转移能力的实验研究[J].天津医科大学学报,2016,22(01):13-16.
 WANG Jin-miao,JIANG Tao,QI Feng,et al.Experimental research on reducing invasion and metastasis ability of HCT-116 by inhibiting microRNA-21 expression[J].Journal of Tianjin Medical University,2016,22(01):13-16.
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抑制microRNA-21表达降低HCT-116细胞侵袭转移能力的实验研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
22
期数:
2016年01
页码:
13-16
栏目:
基础医学
出版日期:
2016-01-20

文章信息/Info

Title:
Experimental research on reducing invasion and metastasis ability of HCT-116 by inhibiting microRNA-21 expression
文章编号:
1006-8147(2016)01-0013-04
作者:
王金淼姜 涛戚 峰刘 彤王鹏志
(天津医科大学总医院普通外科,天津300052)
Author(s):

WANG Jin-miao JIANG TaoQI FengLIU TongWANG Peng-zhi

(Department of General Surgery,General Hospital,Tianjin Medical University,Tianjin 300052,China)
关键词:
结肠癌侵袭转移microRNA-21质粒
Keywords:
colonic cancer invasion metastasis microRNA-21 plasmid
分类号:
R735.3+5
DOI:
-
文献标志码:
A
摘要:
目的:探讨抑制人结肠癌细胞HCT-116 microRNA-21表达降低结肠癌细胞侵袭转移能力作用机制。方法:应用表达反义microRNA-21的质粒miRZip21转染HCT-116细胞,检测microRNA-21的变化,TargetScan和PicTar等工具筛选与侵袭转移相关的靶基因,实时定量PCR方法检测转染前后靶基因mRNA的变化,Western Blot检测靶基因蛋白表达变化,平皿划痕和Transwell实验观察抑制microRNA-21与否HCT-116细胞转移和侵袭能力。结果:筛选出4种与侵袭转移相关的microRNA-21靶基因:TIMP-3,RECK,BMPRⅡ和PCDH17;miRZip21质粒转染HCT-116后,micro-RNA 21表达水平下降约40%,靶基因TIMP-3和RECK 的mRNA及靶蛋白表达上升明显,有统计学意义(P <0.05);平皿划痕和Transwell实验结果表明抑制microRNA-21后,HCT-116细胞转移侵袭能力明显减弱,差异有统计学意义(P <0.05)。结论:抑制microRNA-21的表达能够减弱HCT-116细胞的侵袭和转移能力。
Abstract:
Objective: To explore the effect on invasion and metastasis of HCT-116 colon cancer cell by downregulating the microRNA-21 expression. Methods: After plasmid miRZip21, miRZipTManti-microRNA-21expression lentivector , was used to transfect HCT-116, the expression of microRNA-21 was assessed. The microRNA-21 target genes associated with invasion and metastasis were predicted by TargetScan and PicTar. The mRNA and protein of taget genes were detected by qRT-PCR and Western blotting respectively. The ability of migration and invasion of HCT-116 after transfection were detected by the Scratch assay and Transwell assay. Results: TIMP-3, RECK, BMPRⅡ and PCDH17 were the gene targets associated with invasion and metastasis; After the cells were transfected with plasmid miRZip21,the microRNA-21 level descended to about 60%,the mRNA and protein level of TIMP-3 and RECK increased significantly (P<0.05); Significant reduction of invasion and migration were observed in anti-microRNA-21-transfected HCT-116 (P <0.05). Conclusion: Inhabiting the microRNA-21 expression can lessen the invasive and metastatic ability of HCT-116 cells.

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备注/Memo

备注/Memo:

基金项目 天津市自然科学基金资助项目(08JCYBJC08100)

作者简介 王金淼(1987-),男,硕士在读,研究方向:普通外科;通信作者:戚峰,E-mail:qf@medmail.com.cn

更新日期/Last Update: 2016-01-25