|本期目录/Table of Contents|

[1]刘春利,刘晓伟,肖丹娜,等.牵张力对C2C12细胞株MEF2A基因表达的影响[J].天津医科大学学报,2015,21(04):296-298.
　LIU Chun-li,LIU Xiao-wei,XIAO Dan-na,et al.Effects of C2C12 on cell lines MEF2A gene expression[J].Journal of Tianjin Medical University,2015,21(04):296-298.
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牵张力对C2C12细胞株MEF2A基因表达的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年04期
页码:
296-298
栏目:
基础医学
出版日期:
2015-07-15

文章信息/Info

Title:
Effects of C2C12 on cell lines MEF2A gene expression
文章编号:
1006-8147（2015）04-0296-03
作者:
刘春利1; 刘晓伟2; 肖丹娜1; 高 辉1
(1.天津市口腔医院正畸科, 天津300041;2. 武警后勤学院附属医院口腔科, 天津300162)
Author(s):
LIU Chun-li1; LIU Xiao-wei2; XIAO Dan-na1; GAO Hui1
(1. Department of Orthodontics，Tianjin Stomatological Hospital，Tianjin 300041，China；2. Department of Stomatology，Affiliated Hospital of the Armed Police Logistics College，Tianjin 300162,China)
关键词:
 ; C2C12细胞株;  牵张应力;  MEF2A基因
Keywords:
C2C12;  cyclic stretch;  MEF2A
分类号:
R783.5
DOI:
-
文献标志码:
A
摘要:

目的:评价牵张力作用后C2C12细胞MEF2A基因表达变化。方法: 应用四点弯曲加力系统对C2C12细胞施加周期性机械牵张力，加力频率0.5 Hz，形变量2 000μstrain，加力时间分别为1、2、4、8、12 h。采用实时荧光定量PCR检测受力后C2C12细胞MEF2A基因表达变化。结果:对于C2C12细胞在12 h内设定的时间段加0.5 Hz，加力板形变量2 000μstrain的周期性牵张力后，其MEF2A基因表达未因加力明显变化，其表达量的变化未见统计学意义，不同加力时间MEF2A基因表达无明显差异（P>0.05)。结论:短时间加力刺激对骨骼肌的改建影响较小，12 h以内牵张力刺激对颌面部骨骼肌无明显影响。

 

Abstract:
Objective: To evaluate the effect of the cyclic stretch on the expression of MEF2A mRNA. Methods: C2C12 cells were stretched cyclically using a four-point bend device, in different periods of 2000 μstrain periodic mechanical tension. Real-time quantitative PCR were applied to detect the level of MEF2A mRNA. Results：In 12 h within the set periods 0.5 Hz, 2000 μstrain strength added to C2C12 cells, MEF2A gene expression was not obviously affected, and the quantity of expression was not statistically significat(P>0.05). Conclusion: The length of the loading time is one of the most important factors that affect the reconstruction of skeletal muscle, and Pulling tension within 12 h has no significant influence on maxillofacial skeletal muscle reconstruction.

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备注/Memo

备注/Memo:

基金项目 天津市自然科学基金资助项目 ( 06YFJMJC08900 )

作者简介 刘春利（1979-）， 女， 主治医师， 硕士， 研究方向：口腔正畸学；通信作者： 高辉 E-mail ：keyanjiaoxue@gmail.com。

更新日期/Last Update: 2015-07-16