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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

21卷

期数:

2015年04期

页码:

299-303

栏目:

基础医学

出版日期:

2015-07-15

文章信息/Info

Title:

Effects of Porphyromonas gingivalis lipopolysaccharide on osteogenic differentiation of human periodontal ligament cells

文章编号:

1006-8147（2015）04-0299-05

作者:

薛 栋; 蒋少云*; 邓嘉胤; 董允允; 张 蕊

(天津医科大学口腔医院牙周科,天津医科大学口腔医学院 ,天津300070)

Author(s):

XUE Dong;  JIANG Shao-yun;  DENG Jia-yin;  DONG Yun-yun;  ZHANG Rui

(Department of Periodontics, Hospital of Stomatology, School of Dentistry, Tianjin Medical University, Tianjin 300070, China)

关键词:

牙龈卟啉单胞菌; 脂多糖; 人牙周膜细胞; 牙周炎; 成骨分化

Keywords:

Porphyromonas gingivalis; lipopolysaccharide; human periodontal ligament cells; periodontitis;  osteogenic differentiation

分类号:

R781.4

DOI:

-

文献标志码:

A

摘要:

目的：观察牙龈卟啉单胞菌（P.g）脂多糖（LPS）对人牙周膜细胞（HPDLCs）成骨分化的影响，探索其作用机制。方法：I型胶原酶+组织块法获得HPDLCs后，分3组培养细胞：α-MEM培养（A组）、成骨诱导液培养（B组）和1 μg/mL P.g LPS+成骨诱导培养（C组）。MTT法检测3组HPDLCs的增殖能力；碱性磷酸酶染色、茜素红染色及其OD值分析，观察HPDLCs的成骨分化能力；采用实时定量PCR检测第3天、第7天以及第14天成骨相关基因的表达。结果：B组与C组细胞增殖无明显差异（P>0.05）；B组和C组ALP染色及茜素红染色均为阳性，两者茜素红OD值与A组相比明显增高（P<0.05），但C组OD值明显低于B组（P<0.05）；B组与C组中成骨相关基因的表达均较A组升高，但与B组相比，C组成骨相关基因水平下降（P<0.05）。结论：P.g LPS通过抑制成骨相关基因的表达，干扰HPDLCs的成骨分化能力，从而影响牙周组织在成骨方面的自我修复。

Abstract:

Objective：To investigate the influence and mechanism of Porphyromonas gingivalis (P.g) lipopolysaccharide (LPS) on osteogenic differentiation of human periodontal ligament cells (HPDLCs). Methods：HPDLCs were obtained from the primary culture of the tissues，and cultured in alpha-MEM (group A) and osteogenic induced medium (group B), as well as osteogenic induced medium containing 1 μg/mL P.g LPS (group C). The proliferation ability of HPDLSCs was analyzed by MTT assay. Alkaline phosphatase (ALP) staining and Alizarin red staining were applied to detect the mineralization ability. The expression levels of osteogenic differentiation related-genes collagen I (COL1), alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and osteocalcin (OCN) were detected by real-time PCR. Results：No obvious difference in cell proliferation was found between group B and group C (P>0.05). ALP staining and alizarin red staining in group B and group C were positive. Compared with group A, OD values in group B and group C were significantly increased (P<0.05), but the OD value in group C was lower than that in group B (P<0.05). Although the expression of osteogenic differentiation related-genes increased significantly in group B and group C, the levels of these genes were higher in group B than that in group C (P<0.05). Conclusion：P.g LPS has negative effect on osteogenic differentiation ability of HPDLCs through inhibiting the expressions of osteogenic differentiation related-genes, resulting in impaired self-repair of periodontal tissue.

 

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备注/Memo

备注/Memo:

作者简介 薛栋（1987-），女，硕士在读，研究方向：牙周炎发病机制；

通信作者：蒋少云，E-mail：jiangshaoyun11@126.com。

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更新日期/Last Update: 2015-07-16