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[1]田原,马跃美,刘艳荣,等.炎症因子IL-23对黑色素瘤B16F10细胞增殖和侵袭能力的影响[J].天津医科大学学报,2014,20(01):8-010.
　TIAN Yuan,MA Yue-mei,LIU Yan-rong,et al.Effects of IL-23 on proliferation and invasion of B16 melanoma cells[J].Journal of Tianjin Medical University,2014,20(01):8-010.
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炎症因子IL-23对黑色素瘤B16F10细胞增殖和侵袭能力的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20卷
期数:
2014年01期
页码:
8-010
栏目:
基础医学
出版日期:
2014-01-20

文章信息/Info

Title:
Effects of IL-23 on proliferation and invasion of B16 melanoma cells
文章编号:
1006-8147(2014)01-0008-03
作者:
田原1; 马跃美1; 刘艳荣2; 房东亮1; 赵秀兰2
（天津医科大学1.外科手术学教研室；2.病理学教研室，天津 300070）
Author(s):
TIAN Yuan 1; MA Yue-mei1;  LIU Yan-rong2; FANG Dong-liang1;  ZHAO Xiu-lan2
(1.Department of Operative Surgery ；2.Department of Pathology, Tianjin Medical University, Tianjin 300070, China)
关键词:
IL-23; B16细胞; 黑色素瘤; 基质金属蛋白酶; 侵袭; NF-kB; 小鼠
Keywords:
IL-23;  melanoma B16 cells;  matrix metalloproteinase;  invasion;  NF-B;  mice
分类号:
R730.23+R739.5
DOI:
-
文献标志码:
A
摘要:

目的： 探讨白细胞介素 -23 对黑色素瘤 B16F10 细胞增殖和侵袭能力的影响。方法：体外培养小鼠黑色素瘤 B16F10 细胞，采用 MTT 法检测 IL-23 对 B16 细胞增殖能力的影响； Transwell 侵袭实验检测 IL-23 对 B16 细胞侵袭能力的影响；明胶酶谱法分析 IL-23 处理后肿瘤细胞 B16 细胞分泌 MMP-2 和 MMP-9 的活性表达情况。免疫印迹杂交法 (Western blot) 检测 IL-23 处理组肿瘤细胞核转录因子κ B P65 （ NF- κ B P65 ）的表达水平。结果：与对照组相比， IL-23 处理组 B16F10 细胞穿膜数明显增多（ P <0.01 ） ； 肿瘤细胞 MMP-2 和 MMP-9 的表达活性增强（ P <0.01 ）；核转录因子 NF- κ B P65 的表达水平显著提高（ P <0.01 ）； B16 细胞的增殖能力未见明显变化（ P >0.05 ） 结论： IL-23 对黑色素瘤 B16F10 细胞的增殖能力无影响，可通过上调 MMP-2 和 MMP-9 的活性从而促进肿瘤细胞侵袭，其分子机制可能与 NF- κ B 信号通路的激活相关。


Abstract:
Objective: To investigate the effects of interlukin-23 (IL-23) on the proliferation and invasion of mouse B16F10 melanoma cells. Methods: Mouse B16 melanoma cells were cultured in vitro. Cells in research were treated with and without a certain-concentration IL-23 respectively. The proliferation of B16 cells was determined by MTT. And the invasion of B16 cells was detected by Transwell experiment. The expression activities of MMP-2 and MMP-9 were analyzed by Gelatin Zymography and NF-κB phosphorylated P65 expression was measured by Western blot. Results: The growth rate of B16 cells treated with IL-23 did not show significant change (P>0.05) compared with the control group. Matrigel invasion assay revealed that addition of IL-23 to B16 cells significantly enhanced its invasive properties (P<0.01), and Gelatin Zymography analysis showed that the MMP-9 activity of B16F10 cells was significantly increased by IL-23 (P<0.01). IL-23 induced an increased NF-κB phosphorylated P65 expression in B16 cells. Conclusion: IL-23 brings no effect on proliferation of B16F10 cells but can promote invasion which is associated with the increased MMP-9 activities, and this process is correlated with NF-κB signal pathway activation.

参考文献/References:

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[6] 房东亮, 马跃美, 赵秀兰, 等. 慢性炎症对小鼠黑色素瘤血管生成的影响[J]. 天津医药,2013,03:230

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[8] Li J, Lau G K, Chen L, et al. Interleukin 17A promotes hepatocellular carcinoma metastasis via NF-kB induced matrix metalloproteinases 2 and 9 expression[J]. PloS One,2011,6(7):e21816

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相似文献/References:

[1]文明明,马跃美,赵秀兰,等.炎症因子IL-23 对黑色素瘤细胞增殖侵袭及双向分化的影响[J].天津医科大学学报,2017,23(04):295.
　WEN Ming-ming,MA Yue-mei,ZHAO Xiu-lan,et al.Effects of IL-23 on the herin proliferation，invasion and bi-differentiation? of melanoma cells[J].Journal of Tianjin Medical University,2017,23(01):295.

备注/Memo

备注/Memo:
基金项目 天津市自然科学基金资助项目（10JCZDJC20400）
作者简介 田原（1987-），男，硕士在读，研究方向：慢性炎症与肿瘤生成；
通信作者：马跃美，Email:mindyma@126.com。
更新日期/Last Update: 2014-03-24