|本期目录/Table of Contents|

[1]徐惠君,贾勇圣,陈 悦,等.小白菊内酯对人乳腺癌细胞株增殖敏感性的影响[J].天津医科大学学报,2014,20(01):11-013.
 XU Hui-jun,JIA Yong-sheng,CHEN Yue,et al. Inhibition effect of parthenolide on the human breast cancer BT549 cells[J].Journal of Tianjin Medical University,2014,20(01):11-013.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20
期数:
2014年01期
页码:
11-013
栏目:
基础医学
出版日期:
2014-01-20

文章信息/Info

Title:
 Inhibition effect of parthenolide on  the human breast cancer BT549 cells
文章编号:
1006-8147(2014)01-0011-03
作者:
 徐惠君1贾勇圣1陈 悦2佟仲生1
 (1. 天津医科大学肿瘤医院乳腺内科,国家肿瘤临床医学研究中心,乳腺癌防治教育部重点实验室,天津市肿瘤防治重点实验室,天津 300060; 2. 南开大学药学院,天津 300071)
Author(s):
 XU Hui-jun1 JIA Yong-sheng1 CHEN Yue2 TONG Zhong-sheng1
 (1. Department of Breast Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China; 2. College of Pharmacy, Nankai University, Tianjin 300071, China )
关键词:
小白菊内酯顺铂乳腺癌细胞细胞周期
Keywords:
 parthenolide cis-platinum breast cancer cells cell cycle
分类号:
R696
DOI:
-
文献标志码:
A
摘要:
 目的:观察小白菊内酯 ( PN )单独及联合顺铂(DDP)对人乳腺癌细胞株BT549生长的影响,并探讨其作用机制。方法:体外培养人乳腺癌BT549细胞株,分别加入不同浓度的PN、DDP及PN+DDP至细胞中,作用48 h后,分别采用CCK8细胞增殖实验检测细胞存活率,流式细胞仪检测细胞周期。结果:随着PN工作液浓度的升高,BT549细胞48 h的细胞存活率逐渐降低。同样,随着DDP工作浓度的升高,BT549细胞48 h的细胞存活率也逐渐下降。而相应浓度的PN和DDP联合作用于BT549细胞48 h的存活率降低更加明显,且差异具有统计学意义(P<0.05)。流式测细胞周期实验发现,这两种药物主要通过阻滞细胞周期G1/S期的转化。结论:PN单用及联合DDP作用均可抑制BT549细胞的增殖,且呈浓度-剂量依赖性,两药联合应用对细胞增殖的抑制作用更加明显,表明这两种药物具有协同作用。
Abstract:
 Objective: To observe the effect of parthenolide (PN) alone and in combination with cis-platinum (DDP) on the growth of human breast cancer cells BT549 and its mechanism. Methods:Human breast cancer BT549 cells were cultured in vitro. Different concentrations of PN, DDP and PN combined with DDP were added to BT549 cells. After 48 h, the cell viability rate was detected by CCK8 assay. Cell cycle distribution was tested by flow cell cycle experiment. ResultsThe cell viability rate declined after different concentrations of PN were added to BT549 cells in 48 h. And the cell viability rate decreased after different concentrations of DDP were added to BT549 cells in 48 h, and the difference was statistically significant(P<0.05). The cell viability rate sharply reduced after corresponding concentrations of PN combined with DDP were added to BT549 cells in 48 h. Cell cycle G1/S transition was mainly blocked by the two drugs. Conclusion:PN alone and in combination with DDP can both inhibit the proliferation of BTB49 cells in concentration-dose dependant. And the drugs also showed synergistic effect.

参考文献/References:

 [1] Kishida Y, Yoshikawa H, Myoui A. Parthenolide, a natural inhibitor of Nuclear Factor-kappaB, inhibits lung colonization of murine osteosarcoma cells[J]. Clin Cancer Res, 2007, 13 (1): 59

[2] 王秀英, 冷水龙. 小白菊内酯增强肝癌细胞对顺铂敏感性的实验研究[J]. 实用医学杂志, 2011, (272): 177

[3] 余南荣, 李昌宾, 纪术峰, 等. 小白菊内酯及顺铂对人结肠癌细胞SW620凋亡和增殖的作用[J]. 肿瘤学杂志, 2013, 19(4): 277

[4] Gopal Y N, Arora T S, Van Dyke M W. Parthenolide specifically depletes histone deacetylase 1 protein and induces cell death through ataxia telangiectasia mutated[J]. Chem Biol, 2007, 14 (7): 813

[5] Kim J H, Liu L, Lee S O, et al. Susceptibility of cholangiocarcinoma cells to parthenolide-induced apoptosis[J]. Cancer Res, 2005, 65 (14): 6312

[6] Pajak B, Gajkowska B, Orzechowski A. Molecular basis of parthenolide-dependent proapoptotic activity in cancer cells[J]. Folia Histochem Cytobiol, 2008, 46 (2): 129

[7] Zhang D, Qiu L, Jin X, et al. Nuclear factor-kappaB inhibition by parthenolide potentiates the efficacy of Taxol in non-small cell lung cancer in vitro and in vivo[J]. Mol Cancer Res, 2009, 7 (7): 1139

[8] Allday M J, Inman G J, Crawford D H, et al. DNA damage in human B cells can induce apoptosis, proceeding from G1/S when p53 is transactivation competent and G2/M when it is transactivation defective[J]. EMBO J, 1995, 14 (20): 4994

[9] Levy D T, Ross H, Powell L, et al. The role of public policies in reducing smoking prevalence and deaths caused by smoking in Arizona: results from the Arizona tobacco policy simulation model[J]. J Public Health Manag Pract, 2007, 13 (1): 59

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备注/Memo

备注/Memo:
基金项目 天津市重大课题专项基金资助项目(12ZCDZSY16200)
作者简介 徐惠君(1987-),女,硕士在读,研究方向:乳腺癌的综合治疗;通信作者:佟仲生, E-mail:Tonghang@medmail.com.cn。
更新日期/Last Update: 2014-03-25