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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

27卷

期数:

2021年05期

页码:

477-481

栏目:

基础医学

出版日期:

2021-09-10

文章信息/Info

Title:

Effects of NFATc1 on the proliferation, migration and epithelial-mesenchymal transition of non-small cell lung cancer

文章编号:

1006-8147(2021)05-0477-05

作者:

李智慧; 姚菲菲; 王富霞

（郑州人民医院呼吸内科，郑州⁴⁵0000）

Author(s):

LI Zhi-hui; YAO Fei-fei; WANG Fu-xia

（Department of Respiratory Medicine，Zhengzhou People′s Hospital，Zhengzhou 450000，China）

关键词:

活化T细胞c1的核因子; 增殖; 上皮间质转化

Keywords:

nuclear factor of activated T cell c1;  proliferation;  epithelial-mesenchymal transition

分类号:

R734.2

DOI:

-

文献标志码:

A

摘要:

目的：检测活化T细胞c1核因子（NFATc1）对非小细胞肺癌（NSCLC）细胞增殖、迁移和上皮间充质转化（EMT）的影响。方法：qRT-PCR检测NSCLC细胞H1650、H460、H1299及正常人支气管上皮细胞BEAS-2B中NFATc1表达水平，将si-NFATc质粒转染至H1650细胞中，通过CCK-8增殖及集落形成实验、划痕和Western 印迹实验分别检测转染后H1650细胞增殖、迁移及EMT的影响。结果：NFATc1 mRNA在H1650、H460、H1299细胞中的相对表达水平高于BEAS-2B细胞（t=10.732，P<0.001；t=4.705，P=0.009；t=4.792，P=0.009），且H1650细胞中NFATc1 mRNA表达水平高于H460、H1299细胞（t=3.593，P=0.023；t=7.505，P=0.002）；转染si-NFATc1组NFATc1 mRNA（t=9.325，P<0.001），NFATc1蛋白（t=10.254，P<0.001），细胞的OD值（t=7.954，P<0.001）均低于si-NC组，细胞集落形成数（t=12.210，P<0.001），迁移率（t=8.951，P<0.001）低于si-NC组；si-NFATc1组E-cadherin蛋白表达水平高于si-NC组（t=6.352，P<0.001），Vimentin和N-cadherin蛋白的表达（t=6.012，P<0.001；t=10.241，P<0.001）低于si-NC组。结论：NFATc1在H1650细胞中表达水平增加，敲低其表达水平后可减弱细胞增殖、迁移和EMT。

Abstract:

Objective： To detect the effect of activated T cell c1 nuclear factor（NFATc1） on the proliferation，migration and epithelial-mesenchymal transition（EMT） of non-small cell lung cancer（NSCLC） cells. Methods: qRT-PCR was used to detect the expression level of NFATc1 in NSCLC cells H1650，H460，H1299 and normal human bronchial epithelial cells BEAS-2B. The si-NFATc plasmid was transfected into H1650 cells，and the effects of H1650 cell proliferation，migration and epithelial-mesenchymal transition（EMT）were detected by CCK-8 proliferation，colony formation experiments，scratch and Western blotting. Results: The relative expression level of NFATc1 mRNA in H1650，H460，and H1299 cells were higher than those in BEAS-2B cells（t=10.732，P<0.001；t=4.705，P=0.009；t=4.792，P=0.009），and the expression level of NFATc1 mRNA of H1650 cells was higher than that of H460 and H1299 cells（t=3.593，P=0.023；t=7.505，P=0.002）. The NFATc1 mRNA（t=9.325，P<0.001），and the gray value of NFATc1 protein（t=10.254，P<0.001），the OD value of cells （t=7.954，P<0.001）in the si-NFATc1 group were lower than those of the si-NC group，the number of colony formation （t=12.210，P<0.001），and the migration rate in the si-NFATc1 group were lower than those of the si-NC group（t=8.951，P<0.001）. The expression of E-cadherin protein in the si-NFATc1 group was higher（t=6.352，P<0.001），and the expression of Vimentin and N-cadherin protein were lower than those in the si-NC group（t=6.012，P<0.001； t=10.241，P<0.001）. Conclusion: The expression level of NFATc1 increases in H1650 cells，knocking down its expression level can attenuate cell proliferation，migration and EMT.

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备注/Memo

备注/Memo:

作者简介 李智慧（1987-），女，主治医师，硕士，研究方向：肺癌、肺部感染。

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更新日期/Last Update: 2021-09-01