|本期目录/Table of Contents|

[1]闫晓芳,谢 虹,汤 华.miR-3685通过靶向CTTN抑制宫颈癌细胞的迁移、侵袭及生长[J].天津医科大学学报,2020,26(01):8-12.
 YAN Xiao-fang,XIE Hong,TANG Hua.miR-3685 inhibits migration, invasion and growth of cervical cancer cells by targeting CTTN[J].Journal of Tianjin Medical University,2020,26(01):8-12.
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miR-3685通过靶向CTTN抑制宫颈癌细胞的迁移、侵袭及生长(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
26卷
期数:
2020年01期
页码:
8-12
栏目:
基础医学
出版日期:
2020-04-06

文章信息/Info

Title:
miR-3685 inhibits migration, invasion and growth of cervical cancer cells by targeting CTTN
文章编号:
1006-8147(2020)01-0008-05
作者:
闫晓芳谢 虹汤 华
(天津医科大学基础医学院病原生物学系,天津市生命科学中心实验室,天津市炎症生物学重点实验室,天津 300070)
Author(s):
YAN Xiao-fangXIE HongTANG Hua
(Department of Pathogenic Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin Life Science Research Center, Tianjin Key Laboratory of Inflammation Biology, Tianjin 300070, China )
关键词:
miR-3685宫颈癌CTTN上皮间质转化细胞周期
Keywords:
miR-3685 cervical cancer CTTN EMT cell cycle NA Not available
分类号:
R737.33
DOI:
-
文献标志码:
A
摘要:
目的:微小RNA (microRNA,miRNA)通常通过与其靶基因的3′非翻译区(3′UTR)结合而调控肿瘤细胞的恶性行为,目前miR-3685对肿瘤细胞恶性行为的影响鲜有报道,本文旨在探讨miR-3685对宫颈癌细胞恶性行为及其分子机制的影响。方法:生物信息学软件预测miR-3685的靶基因,用EGFP报告系统验证。实时荧光定量PCR(RT-qPCR)技术检测miR-3685和CTTN(cortactin)在人类宫颈癌细胞中的表达,用transwell迁移/侵袭实验、Western blot实验、集落形成实验、细胞周期进程实验分别检测了miR-3685和CTTN对HeLa细胞和SiHa细胞的迁移、侵袭能力、EMT进程、细胞增殖能力及细胞周期进程的影响。结果:与对照组相比,过表达miR-3685可抑制宫颈癌HeLa和SiHa细胞的迁移、侵袭及生长,封闭miR-3685,得到相反的结果。过表达CTTN可促进宫颈癌HeLa和SiHa细胞的迁移、侵袭及生长,EGFP报告系统验证CTTN是miR-3685的直接靶基因(*P<0.05,**P<0.01,***P<0.001)。结论:miR-3685通过抑制CTTN的表达而抑制宫颈癌HeLa和SiHa细胞的迁移、侵袭及生长。
Abstract:
Objective: MicroRNAs(miRNAs) usually regulate the malignant behavior of tumor cells by binding to the 3′ untranslated region(3′UTR) of their target genes. But he effect of miR-3685 on the malignant behavior of cervical cancer cells has not been reported. This study aims to investigate the effect of miR-3685 on the malignant behavior and molecular mechanism of cervical cancer cells. Methods: The bioinformatics software was used to predict the target gene of miR-3685 and verified it with the EGFP reporter system.Real-time quantitative PCR (RT-qPCR) technique was used to detect the expression of miR-3685 and CTTN (cortactin) in human cervical cancer cells.Transwell migration/invasion assay, Western blot assay, colony formation assay and flow cytometry assay were used to detect migration, invasion, EMT progression, cell proliferation and cell cycle process of miR-3685 and CTTN in HeLa cells and SiHa cells. Results: Compared with the control group, overexpression of miR-3685 inhibited migration, invasion and growth of HeLa cells and SiHa cells, while blocking miR-3685 gave the opposite result. Overexpression of CTTN promoted migration and invasion and growth of cervical cancer HeLa and SiHa cells. The EGFP reporter system verified that CTTN is a direct target gene for miR-3685. Conclusion: miR-3685 inhibits the migration, invasion and growth of HeLa cells and SiHa cells by inhibiting the expression of CTTN.

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金重大研究计划集成项目(91629302)
作者简介 闫晓芳(1993-),女,硕士在读,研究方向:病原生物学;通信作者:汤华,E-mail: htang2002@yahoo.com。
更新日期/Last Update: 2020-04-16