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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

22卷

期数:

2016年03期

页码:

185-190

栏目:

基础医学

出版日期:

2016-05-20

文章信息/Info

Title:

Cyclooxygenase-2 promotes gastric cancer invasion and metastasis by inducing epithelial to mesenchymal transition

文章编号:

1006-8147（2016）03-0185-06

作者:

李 静¹; 孙保存¹; 2;  孙 冉³;  赵秀兰¹; 2;  董学易¹; 2; 赵 楠¹; 2 ; 古 强¹; 2 ; 张丹芳¹; 2;  刘铁菊¹; 2

（1.天津医科大学病理学教研室，天津 300070；2.天津医科大学总医院病理科，天津 300052；3.天津市南开医院病理科，天津 300100）

Author(s):

LI Jing¹;  SUN Bao-cun¹; 2;  SUN Ran³;  ZHAO Xiu-lan¹; 2;  DONG Xue-yi¹; 2;  ZHAO Nan¹; 2;  GU Qiang¹; 2;  ZHANG Dan-fang¹; 2;  LIU Tie-ju¹; 2

(1.Department of Pathology, Tianjin Medical University, Tianjin 300070, China; 2.Department of Pathology, Tianjin Medical University General Hospital, Tianjin 300052, China; 3. Department of Pathology, Tianjin NanKai Hospital, Tianjin 300100, China)

关键词:

胃癌; 环氧合酶2; 上皮间质转换; 细胞迁移; 细胞侵袭

Keywords:

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gastric cancer' target="_blank" rel="external">">gastric cancer;  COX-2;  epithelial-mesenchymal transiton;  migration;  invasion

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分类号:

R735.2

DOI:

-

文献标志码:

A

摘要:

目的：探[1]讨环氧合酶-2（COX-2）及Snail在胃癌组织中的表达及临床意义，研究COX-2转染对人胃癌细胞侵袭迁移能力的影响，并探讨其机制。方法：免疫组化检测COX-2及Snail在100例胃癌组织中的表达情况，分析其与临床资料的关系；筛选构建COX-2稳定表达的MKN74、MKN45细胞系；观察转染前后细胞的形态、功能及相关蛋白的改变。结果: COX-2的表达与远处转移、淋巴结转移、不良预后密切相关（p＜0.05）；Snail的表达与分期、淋巴结转移、远处转移相关（P＜0.05）；COX-2的表达与Snail之间存在相关性（P＜0.01）。与对照组相比，MKN74/COX-2细胞形态呈现EMT形态学改变，E-cadherin表达下调、vimentin表达上调，迁移侵袭能力明显增强；MKN45/shCOX-2细胞形态由长梭形变为铺路石样，E-cadherin表达上调、vimentin表达下调，迁移侵袭能力减弱。结论：COX-2可以通过EMT促进胃癌细胞的侵袭和转移。

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Abstract:

Objective: To determine the expressions of Cyclooxxygenase-2(COX-2) and Snail in gastric cancer, investigate the effect of COX-2 transduction on invasion and migration of gastric cancer cell lines of MKN74 and MKN45, and explore its related mechanism. Methods: The expressions of COX-2 and Snail were examined by immunohistochemical technique. Lentiviruses carrying COX-2 gene were constructed and transducted into MKN74 and MKN45. The changes in morphology, migration , invasion and protein were detected. Results: The expression of COX-2 was positively correlated with metastasis in lymph node and metastasis in distance（P＜0.05). Snail expression was related to stage, lymph node metastasis and distant metastasis in human gastric cancer（P＜0.05）.There was a positive correlation between COX-2 and Snail expressions（P＜0.01）. The MKN74/COX-2 cells showed EMT changes. The migration and invasion abilities were significantly enhanced. Knockdown of COX-2 resulted in inhibited EMT in MKN45 cells. Migration and invasion abilities were significantly receded in MKN45/shCOX-2 cells. Conclusion: COX-2 plays an important role in the origin and development of gastric cancer, COX-2 through EMT promotes the migration and invasion abilities in gastric cancer cells.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金重点项目（81230050）；国家自然科学基金面上项目（81572872）
?作者简介 李静（1986-），女，硕士在读，研究方向：肿瘤血管生成；

通信作者： 孙保存，E-mail：Sunbaocun@aliyun.com。

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更新日期/Last Update: 2016-05-24