|本期目录/Table of Contents|

[1]刘新兰,李淑璟,张海霞.候选抑癌基因RASSF6在胃癌组织中的表达及其与临床病理特征的关系[J].天津医科大学学报,2015,21(04):287-290.
 LIU Xin -lan,LI Shu -jing,ZHANG Hai -xia.Expressions of RASSF6 in gastric cancer and its association with clinicopathologic feature[J].Journal of Tianjin Medical University,2015,21(04):287-290.
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候选抑癌基因RASSF6在胃癌组织中的表达及其与临床病理特征的关系(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年04期
页码:
287-290
栏目:
基础医学
出版日期:
2015-07-15

文章信息/Info

Title:
Expressions of RASSF6 in gastric cancer and its association with clinicopathologic feature
文章编号:
1006-8147(2015)04-0287-04
作者:
刘新兰1 李淑璟2 张海霞2
  (1.宁夏医科大学总医院肿瘤医院肿瘤内科,银川750004;2.宁夏医科大学研究生院, 银川 750004)
Author(s):

LIU Xin -lan1 LI Shu -jing2 ZHANG Hai -xia2

 

( 1.Department of Oncology, Cancer Hospital,General Hospital of Ningxia Medical University, Yinchuan 750004,China; 2. Graduate School, Ningxia Medical University, Yinchuan 750004,China)
关键词:
胃癌RASSF6 临床病理特征
Keywords:
gastric cancer RASSF6   clinicopathologic feature
分类号:
R735.2
DOI:
-
文献标志码:
A
摘要:
 目的 :探讨候选抑癌基因RASSF6在胃癌组织中的表达及其与临床病理特征的关系。方法 :采用组织微阵列技术,通过免疫组化SP法检测100例胃癌组织和癌旁正常组织中RASSF6的表达,实时荧光定量RT-PCR和Western blot法检测20例新鲜胃癌组织和癌旁正常组织中RASSF6mRNA及蛋白的表达情况。结果: (1)胃癌组织中RASSF6阳性表达率明显低于癌旁正常组织( 31.0% vs 76.0%,P<0.05),胃癌组织中RASSF6mRNA和蛋白表达水平明显低于癌旁正常组织(3.949±0.816 vs 4.522±0.685和1.589±2.237 vs 5.723±1.939 ,P均<0.05)。(2)RASSF6在胃癌组织中的表达与胃癌分化程度、浸润深度、淋巴结转移及胃癌分期差异均有统计学意义(P均<0.05)。结论: RASSF6可能是胃癌中新的抑癌基因,其表达缺失与胃癌的发生和进展有关。
Abstract:
 Objective: To detect the expression of RASSF6 in gastric cancer, and to explore its association with clinicopathologic feature. Methods:Using tissue microarray technology, the expressions of RASSF6 in the gastric cancers and the adjacent normal tissue were analyzed by immunohistochemistry(n=100). Using real-time fluorescence quantitative PCR and Western blot ,the expressions of RASSF6 mRNA and protein levels in the gastric cancers and the adjacent normal tissues were analyzed(n=20) . Results:(1) RASSF6 was decreased in gastric cancer tissues as compared to the adjacent normal tissues(31.0% vs 76.0%,p<0.05). RASSF6 mRNA and protein were decreased in gastric cancer tissues as compared to the adjacent normal tissues (3.949±0.816 vs 4.522±0.685 and 1.589±2.237 vs 5.723±1.939,all of p<0.05). (2)The expression of RASSF6 was associated with the differentiation degree, infiltration depth, lymph node metastasis and gastric cancer points , and significant differences were found (p<0.05). Conclusion RASSF6 may be a new tumor suppressor gene in gastric cancer tissue. The loss expression of RASSF6 is associated with occurrence and development of gastric cancer.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 宁夏自然科学基金项目资助(NZ11271)

作者简介 刘新兰(1968-),女,主任医师,教授,硕士,研究方向:实体瘤的化疗和基础研究; E-mail:liuxinlan@csco.org.com。

更新日期/Last Update: 2015-07-16