|本期目录/Table of Contents|

[1]张 洁,张 飞,冀 为,等. SHP2不同突变体对乳腺癌细胞的迁移和侵袭能力的影响[J].天津医科大学学报,2015,21(02):93-96.
　ZHANG Jie,ZHANG Fei,JI Wei,et al. Effect of different SHP2 mutants on breast cancer cell migration and invasion[J].Journal of Tianjin Medical University,2015,21(02):93-96.
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 SHP2不同突变体对乳腺癌细胞的迁移和侵袭能力的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年02期
页码:
93-96
栏目:
基础医学
出版日期:
2015-03-20

文章信息/Info

Title:
 Effect of different SHP2 mutants on breast cancer cell migration and invasion
文章编号:
1006-8147（2015）02-0093-04
作者:
 张 洁; 张 飞; 冀 为; 赵元元; 牛瑞芳
 （天津医科大学肿瘤医院公共实验室，国家肿瘤临床医学研究中心，天津市“肿瘤防治”重点实验室，天津 300060）
Author(s):
 ZHANG Jie;  ZHANG Fei;  JI Wei;  ZHAO Yuan-yuan;  NIU Rui-fang
 (Public Laboratory, Cancer Institute and Hospital, Tianjin Medical University ，National Clinical Research Center of Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy， Tianjin 300060, China)

关键词:
乳腺癌;  SHP2;  磷酸酶活性; 迁移; 侵袭
Keywords:
 ; breast cancer;  SHP2;  phosphatase activity;  invasion;  migration

分类号:
R737.9
DOI:
-
文献标志码:
A
摘要:
 目的：采用慢病毒感染的方式将SHP2基因和SHP2突变体导入乳腺癌细胞系MDA-MB-231中， 研究SHP2及其各突变体对乳腺癌细胞迁移和侵袭能力的影响。方法：构建SHP2-WT和SHP2-T468M、SHP2-N308D和SHP2-2YF 3个突变体的慢病毒载体，完成慢病毒包装后，感染乳腺癌细胞系MDA-MB-231稳定表达。通过蛋白免疫印记检测高表达情况，免疫荧光观察定位情况，划痕、Transwell实验检测细胞的迁移和侵袭能力。结果：SHP2-WT增强了乳腺癌细胞迁移和侵袭能力，SHP2-T468M，SHP2-N308D与对照相比无显著影响，SHP2-2YF与对照相比降低了乳腺癌细胞的迁移和侵袭能力。结论：SHP2-WT能显著增强乳腺癌细胞迁移和侵袭能力，这可能与其磷酸酶活性无关，而与其542位和580酪氨酸的磷酸化有关。
Abstract:
Objective: To explore the effects of SHP2 and its mutants on migration and invasion of breast cancer cells by transfecting SHP2 and SHP2 mutants into breast cancer cell line MDA-MB-231. Methods: First inglentiviral vector SHP2-WT and three mutants of SHP2-T468M, SHP2-N308D and SHP2-2YF were constructed, and  Then the lentiviral packaging was completed to infect breast cancer cell line MDA-MB-231 for stable expression. The expression, location, cell migration and invasion were detected by western blotting, immunofluorescence, wound-healing and transwell assay respectively. Results: Migration and invasion abilities breast cancer cell of were enhanced by SHP2-WT. SHP2-T468M, SHP2-N308D had no significant effect compared with the control group. The SHP2-2YF lessened the migration and invasion in breast cancer cells. Conclusion: SHP2-WT can significantly increase the invasion and migration of breast cancer cells, which may not be related to the phosphatase activity, but associate with 542 and 580 tyrosine phosphorylation .

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备注/Memo

备注/Memo:
 基金项目 国家自然科学基金资助项目（81372844）；教育部博士点基金资助项目(20131202110002)；天津医科大学科研基金资助项目（2009ky21）

作者简介 张洁（1989-），女，硕士在读，研究方向：生物化学与分子生物学；

通信作者：牛瑞芳，E-mail：niuruifang@tjmuch.com。

更新日期/Last Update: 2015-03-24