|本期目录/Table of Contents|

[1]苟 芸,黄国伟,陈 爽,等. 同型半胱氨酸对小鼠成神经瘤细胞毒性作用研究[J].天津医科大学学报,2015,21(01):6-8.
　GOU Yun,HUANG Guo-wei,CHEN Shuang,et al.Neurotoxicity of homocysteine on mouse N2a neuroblastoma cells[J].Journal of Tianjin Medical University,2015,21(01):6-8.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年01期
页码:
6-8
栏目:
基础医学
出版日期:
2015-01-20

文章信息/Info

Title:
Neurotoxicity of homocysteine on mouse N2a neuroblastoma cells
文章编号:
1006-8147（2015）0-0006-03
作者:
苟 芸;  黄国伟;  陈 爽;  张绪梅
 （天津医科大学公共卫生学院营养与食品卫生学系，天津 300070）
Author(s):
GOU Yun;  HUANG Guo-wei;  CHEN Shuang;  ZHANG Xu-mei
(Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin 300070, China)
关键词:
 ; 同型半胱氨酸; 小鼠; 成神经瘤细胞N2a; 细胞凋亡; pERK1/2
Keywords:
homocysteine;  mouse;  N2a;  cell apoptosis;  pERK1/2
分类号:
R15
DOI:
-
文献标志码:
A
摘要:
 目的：研究同型半胱氨酸（Hcy）对小鼠成神经瘤细胞（N2a）毒性作用并探讨其对pERK1/2蛋白表达的影响。 方法： 培养的N2a细胞加入不同浓度Hcy，随机分为4组：分别为正常对照组及Hcy低、Hcy中、Hcy高浓度组，根据预实验结果确定以上4组Hcy干预浓度分别为 0、 30、300和 1 000 μmol/L。作用72 h后，用酶标仪测定细胞培养液中乳酸脱氢酶（LDH）活性，MTT法检测细胞增殖情况，蛋白质免疫印迹法检测N2a细胞pERK1/2蛋白表达水平。 结果： 与正常对照组相比，Hcy低、中、高浓度组细胞培养液中LDH活性均显著升高，差异具有统计学意义（P值分别为0.013、0.008和0.011， P<0.05）。低、中、高浓度Hcy作用后，MTT检测细胞增殖活力下降，OD值降低，与对照组比较，差异具有统计学意义（P值分别为0.01、0.006和0.009， P<0.05）。各Hcy干预组pERK1/2蛋白表达量降低，且中、高浓度Hcy组pERK1/2蛋白表达量与对照组相比差异具有统计学意义（P值分别为0.038、0.007， P<0.05）。 结论： Hcy能抑制磷酸化的ERK1/2蛋白表达，从而对N2a产生毒性作用，进而抑制N2a增殖，提示降低血清中Hcy水平可以对神经细胞产生保护作用。
Abstract:
Objective: To explore the neurotoxicity of homocysteine on mouse neuroblastoma cells (N2a) and its effect on pERK1/2 protein level. Methods: Cultured N2a cells were divided into four groups according to the concentrations of homocysteine: control group (0 μmol/L), low-Hcy (30 μmol/L), moderate-Hcy (300 μmol/L), high-Hcy (1 000 μmol/L) groups. After 72 h Hcy treatment, the toxicity of N2a cells was detected by LDH (lacate dehydrogenase) assay kit, cell proliferation ability was detected by MTT methods, and the protein expression of pERK1/2 was detected by western blot. Results: The LDH activity in Hcy-treated groups was increased compared with control group (P<0.05). The cells proliferation ability was decreased after Hcy treatment, and OD values were reduced compared with control group (P<0.05). The protein expression of pERK1/2 decreased after Hcy treatment, and significant differences among the moderate, high Hcy dose groups and control group were found (P<0.05). Conclusion: The toxic effect of Hcy on N2a cells may be caused by reduced ERK1/2 protein phosphorylation expression. It suggests that a low serum Hcy level may have a protective effect on neural cells.

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（81373003）； 中国博士后科学基金（2014M550148 )

作者简介 苟芸（1990-），女， 硕士在读，研究方向：营养与神经退行性疾病；通信作者：张绪梅，E-mail: zhangxumei@tijmu.edu.cn 。

更新日期/Last Update: 2015-06-10