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[1]林高阳,徐 克.氯化锂调控人肺腺癌A549细胞增殖侵袭转移的体外研究[J].天津医科大学学报,2015,21(01):9-13.
 LIN Gao-yang,XU Ke.Investigation of the effect of lithium chloride on proliferation and metastasis potential of human lung adenocarcinoma A549 cells in vitro[J].Journal of Tianjin Medical University,2015,21(01):9-13.
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氯化锂调控人肺腺癌A549细胞增殖侵袭转移的体外研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21
期数:
2015年01期
页码:
9-13
栏目:
基础医学
出版日期:
2015-01-20

文章信息/Info

Title:
Investigation of the effect of  lithium chloride on proliferation and  metastasis potential of human lung adenocarcinoma A549  cells  in vitro
文章编号:
1006-8147(2015)0-0009-05
作者:
林高阳徐 克
(天津医科大学总医院肺部肿瘤外科,天津市肺癌转移与肿瘤微环境重点实验室,天津 300052)
Author(s):
LIN Gao-yang XU Ke
(Department of Lung Cancer Surgery,General Hospital,Tianjin Medical University,Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment,Tianjin 300052,China)
关键词:
肺腺癌氯化锂迁移侵袭  GSK-3B/B-catenin通路
Keywords:
lung adenocarcinoma lithium chloride metastasis/invasion GSK-3B/B-catenin signaling pathway
分类号:
R734.2
DOI:
-
文献标志码:
A
摘要:
目的:探讨氯化锂对人肺腺癌A549细胞增殖和侵袭转移能力的影响及其调控机制。方法:应用MTT法、平板克隆增殖实验评价细胞生长活力和增殖的生物学特征变化;划痕实验、Transwell小室细胞体外侵袭实验检测肿瘤细胞迁移、侵袭能力;Western-blotting法检测肿瘤细胞GSK-3b、P-GSK-3bB和B-catenin的表达水平变化。结果MTT法显示细胞分化增殖活力随氯化锂浓度的增加受到明显抑制,具有浓度依赖性变化。同时,随着检测时间的延长,在低浓度药物作用下(<10 mmol/L)细胞出现增殖强化现象,可能存在药物时间耐受;平板克隆增殖实验显示细胞增殖受到不同浓度氯化锂的抑制,增殖能力的变化亦具有氯化锂浓度依赖性划痕实验、Transwell实验证实:氯化锂处理细胞后,细胞迁移、侵袭能力下降;Western-blotting法显示:氯化锂处理肺腺癌A549细胞后,GSK-3B总蛋白表达下降、P-GSK-3B表达增加,b-catenin总蛋白表达增多。结论:高浓度的氯化锂能够抑制肺腺癌A549细胞的增殖侵袭和迁移,氯化锂抑制肺腺癌A549细胞增殖侵袭和迁移可能是通过GSK-3B/B-catenin信号通路实现的。 
Abstract:
 Objective:To investigate the impact of lithium chloride on the proliferation and invasion abilities of human lung cancer cell A549. Methods:Cell growth and proliferation were determined by MTT method and Colony Proliferation Assay. Migration and invasion abilities of human lung cancer cell A549 were detected by wound scratch assay and Transwell assay. The expression of GSK-3b (Glycogen synthase kinase-3b), P-GSK-3b (Phosphorylation-GSK-3b) and b-catenin of human lung cancer cell A549 were detected by western-blotting. Results The proliferation of A549 was significantly decreased after treated with lithium chloride indicating a lithium chloride concentration-dependent manner. Meanwhile, with the extension of the detection time, enhanced cell proliferation phenomenon was observed at low concentrations (<10 mmol/L), indicating a time-tolerance of the drug. The proliferation of A549 could also be inhibited by lithium chloride with a dose-dependent manner. The migration and invasion capabilities of A549 decreased after treated with lithium chloride. And It was also found that the expression of GSK-3b was decreased while the expression of P-GSK-3b and b-catenin were increased after lithium chloride treatment. Conclusion:High concentration of lithium choride could inhibit the proliferation and invasion of A549, and this may be acheived by GSK-3b/b-catenin signaling pathway.

参考文献/References:

is[1]Jemal A, Bray F, Center M M, et al. Global Cancer statistics[J]. CA Cancer J Clin, 2011,61(2):69

[2]Nguyen D X, Massague J. Genetic determinants of Cancer metastasis[J]. Nat Rev Genet, 2007,8(5):341

[3]D’souza R. Rajji TK,mulsant BH.pollock BG use of Lithium in the treatment of bipolar disorder in late-life[J]. Curr Psychiatry Rep J, 2011,13(6):488

[4]Filyak Y, Filyak O, Stoika R. Transforming growth factor beta-1 enhances cytotoxic effect of doxorubicin in human lung adenocarcinoma cells of A549 line[J]. Cell Biol Int, 2007,31(8):851

[5]Lan Y, Liu X, Zhang R, et al. Lithium enhances TRAIL-induced apoptosis in human lung carcinoma A549 cells[J]. Biometals, 2013,26(2):241

[6]Hager E D, Dziambor H, Höhmann D, et al. Effects of Lithium on thrombopoiesis in patients with low platelet cell counts following chemotherapy or radiotherapy[J]. Biol Trace Elem Res, 2001,83(2):139

[7]Hager E D, Dziambor H, Winkler P, et al. Effects of Lithium carbonate on hematopoietic cells in patients with persistent neutropenia following chemotherapy or radiotherapy[J]. Journal of Trace Elements in Medicine and Biology, 2002,16(2):91

[8]Gupta A, Schulze T G, Nagarajan V, et al. Interaction networks of Lithium tas

[9]Beurel E, Blivet-Van Eggelpoël M J, Kornprobst M, et al. Glycogen synthase kinase-3 inhibitors augment TRAIL-induced apoptotic death in human hepatoma cells[J]. Biochem Pharmacol, 2009,77(1):54

[10]Liao X, Zhang L, Thrasher J B, et al. Glycogen synthase kinase-3beta suppression eliminates tumor necrosis factor-related apoptosis-inducing ligand resistance in prostate Cancer[J]. Mol Cancer Ther, 2003,2(11):1215

[11]Mego M, Mani S A, Cristofanilli M. Molecular mechanisms of metastasis in breast cancer--clinical applications[J]. Nat Rev Clin Oncol, 2010,7(12):693

[12]Yamamoto H, Yoo S K, Nishita M, et al. Wnt5a modulates glycogen synthase kinase 3 to induce phosphorylation of receptor tyrosine kinase Ror2[J]. Genes Cells, 2007,12(11):1215

[13]Zhan G F, Phiel C J, Spece L, et al. Inhibitory phosphorylation of glycogen synthase kinase-3(GSK-3)[J]. J Biol Chem, 2003,278(35):33067

[14]Gould T D, Gray N A, Manji H K. Effects of a glycogen synthase kinase-3 inhibitor, Lithium, in adenomatous polyposis coli mutant mice[J]. Pharmacol Res, 2003,48(1):49

[15]Beurel E, Kornprobst M, Blivet-Van Eggelpoël M J, et al. GSK-3beta inhibition by Lithium confers resistance to chemotherapy-induced apoptosis through the repression of CD95 (Fas/APO-1) expression[J]. Exp Cell Res, 2004,300(2):354

相似文献/References:

[1]林高阳,徐 克.氯化锂调控人肺腺癌A549细胞增殖侵袭转移的体外研究[J].天津医科大学学报,2015,21(03):9.
 LIN Gao-yang,XU Ke.Proliferation and invasion potentials of lung adenocarcinoma cell A549 regulated by lithium chloride in vitro[J].Journal of Tianjin Medical University,2015,21(01):9.
[2]赵青春,韦 森,张洪兵,等.分析第6版和第7版TNM分期在肺腺癌患者中的差异[J].天津医科大学学报,2015,21(05):432.

备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目(30873035);天津市教委重点资助项目(ZD200714);天津市科委重点资助项目(10JCZDJC20800)

作者简介 林高阳(1988-),男,硕士在读,研究方向:肺癌的侵袭转移;

通信作者:徐克,E-mail:lingaoyang1988@126.com。

更新日期/Last Update: 2015-06-10