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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

26卷

期数:

2020年05期

页码:

412-417，433

栏目:

基础医学

出版日期:

2020-09-20

文章信息/Info

Title:

Effect of HP1α on the migration, invasion and proliferation of prostate cancer cells

文章编号:

1006-8147(2020)05-0412-07

作者:

张泗扬; 徐勇

（天津医科大学第二医院泌尿外科，天津市泌尿外科研究所，天津 300211）

Author(s):

ZHANG Si-yang;  XU Yong

（Department of Urology，The Second Hospital，Tianjin Medical University， Tianjin Institute of Urology，Tianjin 300211，China）

关键词:

前列腺癌; HP1α; 细胞迁移; 侵袭; 增殖; EMT

Keywords:

prostate cancer;  HP1α; cell migration; invasion; proliferation; EMT

分类号:

R737.25

DOI:

-

文献标志码:

A

摘要:

目的：探讨HP1α在前列腺癌细胞LNCaP和PC3中的表达情况，并研究HP1α的表达对LNCaP和PC3的影响。方法：采用qPCR及Western 印迹检测HP1α的表达情况，通过Transwell检测细胞的迁移和 侵袭能力，CCK-8和集落形成检测细胞的增殖能力。结果：HP1α在前列腺癌细胞C4-2和LNCaP中表达水平最高，PC3和DU145次之，良性前列腺增生的最低（P <0.05）；Transwell显示，与si-NC组相比， si-HP1α组前列腺癌细胞LNCaP和PC3的迁移和侵袭能力均降低（P <0.05）；CCK-8和集落形成实验显示敲低HP1α后，前列腺癌细胞LNCaP和PC3的增殖能力下降（P <0.05）；Western 印迹发现EMT分子标 志物E-cadherin的表达增加，而N-cadherin、Vimentin等的表达均降低（均P <0.05）。结论：HP1α在前列腺癌细胞LNCaP、C4-2、PC3和DU145中高表达，而降低其表达可以抑制前列腺癌细胞LNCaP和PC3 的迁移、侵袭、增殖以及上皮-间充质转化（EMT）能力。

Abstract:

Objective: To investigate the expression of HP1α in prostate cancer cells LNCaP and PC3,and the effect of the expression of HP1α on LNCaP and PC3. Methods: qPCR and Western blot were used to detect the expression of HP1α, cell migration and invasion were detected by Transwell, and cell proliferation was detected by CCK-8 and colony formation. Results: The expression of HP1α were the highest in C4-2 and LNCaP, followed by PC3 and DU145, and the lowest in BPH（P<0.05）. Transwell showed that compared with si-NC group, the migration and invasion ability of cells were reduced in si-HP1α group（P <0.05）. CCK-8 and colony formation experiments showed that compared with si-NC group the proliferation of cells decreased（P<0.05）. Western blot showed that the expression of E-cadherin increased, while the expression of N-cadherin and Vimentin decreased （all P <0.05）. Conclusion: HP1α is highly expressed in prostate cancer cells, and decreased expression can inhibit the migration, invasion, proliferation and EMT of prostate cancer cells.

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备注/Memo

备注/Memo:

基金项目 天津市教委科研计划项目资助（2017KJ208）
作者简介 张泗扬（1993-）,男，硕士在读，研究方向：泌尿外科；
通信作者：徐勇，E-mail：drxuyong@tmu.edu.cn。

常用功能

更新日期/Last Update: 2020-09-18