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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

26卷

期数:

2020年03期

页码:

244-248

栏目:

临床医学

出版日期:

2020-06-10

文章信息/Info

Title:

Effect of CFL1 expression on prostate cancer progression

文章编号:

1006-8147(2020)03-0244-05

作者:

肖龙飞; 彭华红; 杨阔; 王玉琢

（天津医科大学第二医院泌尿外科，天津市泌尿外科研究所，天津 300211）

Author(s):

XIAO Long-fei;  PENG Hua-hong;  YANG Kuo;  WANG Yu-zhuo

（Department of Urology, The Second Hospital, Tianjin Medical University, Tianjin Institute of Urology, Tianjin 300211, China）

关键词:

前列腺癌; CFL1; 细胞迁移; 侵袭; 增殖; 凋亡

Keywords:

prostate cancer; CFL1; cell migration; invasion;  proliferation;  apoptosis

分类号:

R697+.3

DOI:

-

文献标志码:

A

摘要:

目的：探讨CFL1在前列腺癌中的表达情况，并明确其对前列腺癌进展的影响。方法：采用免疫组化、qPCR及Western blot检测CFL1的表达情况，采用Transwell实验检测细胞的迁移及侵袭能力，CCK-8实验检测细胞的增殖能力。结果：在恶性程度高的前列腺癌中，CFL1的蛋白及mRNA表达水平最高，恶性程度低的次之，良性前列腺增生最低（P＜0.05）；Transwell实验表明si-CFL1组较si-NC组，其迁移和侵袭能力均降低（均P＜0.05）；CCK-8实验显示敲低CFL1后，前列腺癌细胞的增殖能力降低（P＜0.05）。结论：CFL1在前列腺癌中高表达，而减低其表达水平可以抑制前列腺癌的进展。

Abstract:

Objective: To investigate the expression of CFL1 in prostate cancer and to determine its effect on the progression of prostate cancer. Methods: The immunohistochemistry, qPCR and Western blot were used to detect the expression of CFL1. Transwell assay was used to detect the migration and invasion ability of cells. CCK-8 assay was used to detect the proliferation of cells. Results: In prostate cancer with high malignancy, CFL1 had the highest protein and mRNA expression levels, followed by low malignancy and benign prostatic hyperplasia（all P＜0.05）. Transwell experiments showed that the migration and invasion ability were decreased in si-CFL1 group compared with si-NC group(P＜0.05). The CCK-8 experiment showed that the proliferation of prostate cancer cells was decreased after CFL1 knockdown(P＜0.05). Conclusion: CFL1 is highly expressed in prostate cancer, and reducing its expression can inhibit the progression of prostate cancer.

参考文献/References:

[1] Attard G, Parker C, Eeles R A, et al. Prostate cancer[J]. Lancet, 2016, 387(10013):70
[2] Van Troys M, Huyck L, Leyman S, et al. Ins and outs of ADF/cofilin activity and regulation[J]. Eur J Cell Biol, 2008, 87(8/9):649
[3] Estornes Y, Gay F, Gevrey J C, et al. Differential involvement of destrin and cofilin-1 in the control of invasive properties of Isrecol human colon cancer cells[J]. Int J Cancer, 2007,121(10):2162
[4] Zhang Y, Tong X. Expression of the actin-binding proteins indicates that cofilin and fascin are related to breast tumour size[J]. J Int Med Res, 2010, 38 (3):1042
[5] Ono S. Regulation of actin filament dynamics by actin depolymerizing factor/cofilin and actin-interacting protein 1: new blades for twisted filament[J]. Biochemistry, 2003, 42(46): 13363
[6] Abe H, Obinata T, Minamide L S, et al. Xenopus laevis actin-depolymerizing factor/cofilin: a phosphorylation-regulated protein essential for development[J]. J Cell Biol, 1996, 132(5): 871
[7] Zhu B, Fukada K, Zhu H, et al. Prohibitin and cofilin are intracellular effectors of transforming growth factor beta signaling in human prostate cancer cells[J]. Cancer Res, 2006, 66(17): 8640
[8] Posadas I, Perez-Martinez F C, Guerra J, et al. Cofilin activation mediates Bax translocation to mitochondria during excitotoxic neuronal death[J]. J Neurochem, 2012, 120(4):515
[9] Wang W, Eddy R, Condeelis J. The cofilin pathway in breast cancer invasion and metastasis[J]. Nat Rev Cancer, 2007, 7(6):429
[10] Yang Z L, Miao X Y, Xiong L, et al. CFL1 and Arp3 are biomarkers for metastasis and poor prognosis of squamous cell/adenosquamous carcinomas and adenocarcinomas of gallbladder[J]. Cancer Invest, 2013, 31(2):132
[11] Perez-Martinez F C, Carrion B, Lucio M I, et al. Enhanced docetaxel-mediated cytotoxicity in human prostate cancer cells through knockdown of cofilin-1 by Carbon nanohorn delivered siRNA[J].Biomaterials, 2012, 33(32):8152
[12] Xiao P, Ma T J, Zhou C W, et al. Anticancer effect of docetaxel induces apoptosis of prostate cancer via the cofilin-1 and paxillin signaling pathway[J]. Mol Med Rep, 2016, 13(5):4079
[13] Lu L, Fu N, Luo X, et al. Overexpression of cofilin 1 in prostate cancer and the corresponding clinical implications[J]. Oncol Lett, 2015, 9(6):2757
[14] Tang G L, Du R Y, Tang Z B, et al. MiRNALet-7a mediates prostate cancer PC-3 cell invasion, migration by inducing epithelial-mesenchymal transition through CCR7/MAPK pathway[J]. J Cell Biochem, 2018, 119(4): 3725
[15] Monet M, Lehen’kyi V, Gackiere F, et al. Role of cationic channel TRPV2 in promoting prostate cancer migration and progression to androgen resistance[J]. Cancer Res, 2010, 70(3): 1225
[16] Leong S, Mckay M J, Christopherson R I. Biomarkers of breast cancer apoptosis induced by chemotherapy and TRAIL[J]. J Proteome Res, 2012, 11(2):1240
[17] Tahtamouni L H, Shaw A E, Hasan M H, et al. Non-overlapping activities of ADF and cofilin-1 during the migration of metastatic breast tumor cells[J]. BMC Cell Biol, 2013, 14(3):45
[18] Maimaiti Y, Jie T, Jing Z, et al. Aurora kinase A induces papillary thyroid cancer lymph node metastasis by promoting cofilin-1 activity[J]. Biochem Biophys Res Commun , 2016, 473(1):212
[19] Wang F, Wu D, Xu Z, Chen J, et al. miR-182-5p affects human bladder cancer cell proliferation, migration and invasion through regulating Cofilin 1[J]. Cancer Cell Int, 2019, 19:42
[20] Zhu B, Fukada K, Zhu H, et al. Prohibitin and cofilin are intracellular effectors of transforming growth factor beta signaling in human prostate cancer cells[J]. Cancer Res, 2006, 66(17): 8640
[21] Perez-Martinez F C, Carrion B, Lucio M I, et al. Enhanced docetaxel-mediated cytotoxicity in human prostate cancer cells through knockdown of cofilin-1 by Carbon nanohorn delivered siRNA[J].Biomaterials, 2012, 33(32):8152

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备注/Memo

备注/Memo:

作者简介 肖龙飞（1993-），男，硕士在读，研究方向：泌尿外科；通信作者：王玉琢，E-mail：460576427@qq.com。

常用功能

更新日期/Last Update: 2020-06-13