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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

25卷

期数:

2019年01期

页码:

54-58

栏目:

临床医学

出版日期:

2019-01-20

文章信息/Info

Title:

Expression of lncRNA-ANRIL in peripheral blood of patients with coronary heart disease and its clinical significance

文章编号:

1006-8147(2019)01-0054-05

作者:

王 娜¹; 2; 张学军¹

（1.天津医科大学免疫学系, 天津300070；2.天津市滨海新区大港医院检验科, 天津 300100）

Author(s):

WANG Na¹; 2;  ZHANG Xue-jun¹

（1. Department of Immunology, Tianjin Medical University, Tianjin 300070，China； 2. Department of Clinical Laboratory, Tianjin Binhai New Area Dagang Hospital, Tianjin 300100, China）

关键词:

冠心病; lncRNA-ANRIL; 9p21; CDKN2A/B

Keywords:

coronary heart disease (CHD);  lncRNA-ANRIL;  9p21;  CDKN2A/B

分类号:

R541.4

DOI:

-

文献标志码:

A

摘要:

目的：研究冠心病患者外周血中lncRNA-ANRIL的表达水平及其临床意义。方法：选取2016年2月-2018年2月于本院心内科住院的冠心病病人231人作为冠心病组，此外纳入447名健康自愿者作为对照组，取两组病人外周血，RtPCR法测定外周血中lncRNA-ANRIL的表达及9p21基因区域3种单核苷酸：rs1333049、rs564398、rs2811712以及CDKN2A、CDKN2B的相对表达量，分析各变量的相关性，分析ANRIL的表达于冠心病诊断中的敏感性与特异性。结果：ROC曲线示：ANRIL（外显子1-2）、ANRIL（外显子17-18）在诊断冠心病方面均具有统计学意义（均P＜0.01）；lnc RNA-ANRIL（外显子1-2）与CDKN2A、CDKN2B表达均呈正相关（均P＜0.05），lncRNA-ANRIL(外显子17-18)与CDKN2A的表达呈正相关（P＜0.000 1）。冠心病组单核苷酸rs1333049等位基因G显著低于对照组（P＜0.05）；冠心病组rs133049等位基因G与ANRIL(外显子1-2)、ANRIL（外显子17-18）的表达均呈正相关（r=0.340 2/0.312 4，均P＜0.05）；冠心病组ANRIL(外显子1-2)与ANRIL（外显子17-18）的相对表达量均明显小于对照组（均P＜0.01），结论：ANRIL可能通过调控CDKN2A/B的表达影响冠心病的发生发展，ANRIL的表达可能受染色体9p21区域单核苷酸rs1333049表达的影响，外周血ANRIL的表达可以作为早期诊断冠心病的特异性指标。

Abstract:

Objective: To explore the expression level of lncRNA-ANRIL in peripheral blood of patients with coronary heart disease and its clinical significance. Methods: Two hundred and thirty-twocoronary heart disease patients were selected as coronary heart disease group from February. 2016 to February. 2018 in our hospital department of cardiology. In addition, 447 healthy volunteers were included as the control group.The peripheral blood of two groups of patients was extracted. The relative expression of lncRNA-ANRIL and 3 single nucleotides in the region of 9p21 gene:rs1333049, rs564398, rs2811712, and CDKN2A, CDKN2B were measured by RtPCR method. The correlationsbetween variableswereanalyzed, and the sensitivity and specificity of the expression of ANRIL in the diagnosis of coronary heart disease were determined. Results: The ROC curve showed that ANRIL (exon 1-2) and ANRIL (exon 17-18) were statistically significant in the diagnosis of coronary heart disease (P<0.01); The expression of LNC RNA-ANRIL (exon 1-2) was positively correlated with the expression of CDKN2A and CDKN2B (P<0.05). LncRNA-ANRIL (exon 17-18) was positively correlated with the expression of CDKN2A (P<0.0001). The SNPs rs1333049 allele G of the coronary heart disease group was significantly lower than that of the control group(P< 0.05), and the rs133049 allele G was positively correlated with the expression of ANRIL (exon 1-2) and ANRIL (exon 17-18) (r=0.340 2/0.312 4, all P<0.05), and the expression of the relative value of ANRIL (exon 1-2) and ANRIL(exon 17-18) in coronary heart disease group was significantly lower than that of the control group(P<0.01). Conclusion: ANRIL may affect the development of coronary heart disease by regulating the expression of CDKN2A/B. The expression of ANRIL may be influenced by the expression of SNPs rs1333049 of the chromosome 9p21 region. The expression of ANRIL in peripheral blood can be used as a specific marker for early diagnosis of coronary heart disease.

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备注/Memo

备注/Memo:

作者简介 王娜（1979-），女，主管技师，硕士在读，研究方向：医学检验；通信作者：张学军，E-mail: xjzh@tmu.edu.cn。

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更新日期/Last Update: 2019-03-01