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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

23卷

期数:

2017年02期

页码:

138-141

栏目:

临床医学

出版日期:

2017-03-20

文章信息/Info

Title:

Caspase activity of pro-inflammatory monocytes in obese patients

文章编号:

1006-8147（2017）02-0138-04

作者:

张诗田¹; 张 达²; 牛文彦¹

（1.天津医科大学免疫系，天津300070；2.天津医院超声科，天津300211）

Author(s):

ZHANG Shi-tian¹;  ZHANG Da²;  NIU Wen-yan¹

(1.Department of Immunology, Tianjin Medical University, Tianjin 300070, China; 2.Department of Ultrasound, Tianjin Hospital, Tianjin 300211, China)

关键词:

肥胖; 空腹血糖; 单核细胞; Caspase

Keywords:

obese;  fast blood glucose;  monocyte;  Caspase

分类号:

R392.1

DOI:

-

文献标志码:

A

摘要:

目的：探讨外周血炎性单核细胞（CD14⁺CD16⁺）的Caspase活性与肥胖相关指标、空腹血糖（FBG）之间的相关性。方法：招募志愿者，测量身高、体质量、腰围，计算体质指数（BMI），血糖仪测定FBG。根据BMI分为3组：正常组、超重组、肥胖组。密度梯度离心提取外周血单个核细胞，流式细胞术检测CD14⁺CD16⁺单核细胞的数量以及细胞内Caspase活性。结果：肥胖组炎性单核细胞Caspase活性显著高于正常组和超重组，炎性单核细胞Caspase活性与BMI、腰围以及空腹血糖正相关。结论：肥胖和高空腹血糖可能在炎性单核细胞Caspase活性升高中发挥作用。

Abstract:

Objective: To explore the correlation between caspase activity of pro-inflammatory monocytes (CD14⁺CD16⁺) and obese parameters, fast blood glucose (FBG). Methods: Volunteers were recruited. Height, weight and waistline were measured and BMI was calculated. FBG was analyzed by glucometer. Volunteers were grouped based on BMI into normal group, overweight group, and obese group. Peripheral blood mononuclear cells were isolated by density gradient centrifugation. The number of CD14⁺CD16⁺ monocytes and the caspase activity in these cells were measured by flow cytometry. Results: Compared with normal and overweight groups, caspase activity of pro-inflammatory monocytes in obese group was significantly higher. Caspase activity of pro-inflammatory monocytes was positively correlated with BMI, waistline and FBG. Conclusion: Obesity and high fast blood glucose may play a role in the increase of caspase activity of pro-inflammatory monocytes.

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备注/Memo

备注/Memo:

基金项目 ?国家自然科学基金资助 （81170740，81161120545）；高等学校博士学科点专项基金资助 （20121202110014）；天津市科委应用基础研究重点项目基金资助（15JCZDJC35500）; 天津市卫计委重点攻关项目基金资助（15KG102）

作者简介 ?张诗田（1990-），女，硕士在读，研究方向：免疫学；通信作者：牛文彦，E-mail:wniu@tmu.edu.cn。.

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更新日期/Last Update: 2017-03-28