|本期目录/Table of Contents|

[1]米合日尼沙·买买提,阿比丹·吐尔汗江,韩莉莉.宫颈腺癌细胞中HPV18-E7与上皮间质转化的关系研究[J].天津医科大学学报,2016,22(06):487-491.
　Miherinisha Maimaiti,Abidan Tuerhanjiang,HAN Li-li.Study on the relationship between HPV18-? E7 and epithelial -mesenchymal transition in cervical? adenocarcinoma cells[J].Journal of Tianjin Medical University,2016,22(06):487-491.
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宫颈腺癌细胞中HPV18-E7与上皮间质转化的关系研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
22卷
期数:
2016年06期
页码:
487-491
栏目:
基础医学
出版日期:
2016-11-20

文章信息/Info

Title:
Study on the relationship between HPV18-? E7 and epithelial -mesenchymal transition in cervical? adenocarcinoma cells
文章编号:
1006-8147（2016）06-0487-5
作者:
米合日尼沙·买买提; 阿比丹·吐尔汗江; 韩莉莉
(新疆维吾尔自治区人民医院妇科,乌鲁木齐 830000)
Author(s):
Miherinisha Maimaiti;  Abidan Tuerhanjiang;  HAN Li-li
(Department of Gynecology, People’s Hospital of Xinjiang Uygur Autonomous Region, Wulumuqi 830000, China)
关键词:
宫颈腺癌' target="_blank" rel="external">">宫颈腺癌; 上皮-间质转化; HPV18-E7
Keywords:
cervical adenocarcinoma; EMT; HPV18-E7
分类号:
R737.33
DOI:
-
文献标志码:
A
摘要:
目 的:探究宫颈腺癌细胞中HPV18-E7与上皮间质化(EMT)之间的关系。方法: 针对HPV18-E7的siRNA转染入人宫颈腺癌HeLa细胞，观察细胞形态变化，并运用Realtime PCR技术、免疫印迹实验和细胞免疫荧光染色实验在mRNA水平和蛋白水平检测E7、EMT相关的标记因子的表达及定位变化；应用细胞划痕愈合实验及Transwell细胞体外侵袭实验检测细胞迁移和侵袭能力。结果: 特异性沉默HPV18-E7表达后HeLa-siE7细胞间连接变得疏松，细胞中E7与间质性标记物的mRNA及蛋白表达水平较对照组细胞显著下降，上皮性标记因子较对照组细胞明显增加（P<0.01）；随着HeLa细胞E7表达的下调，E-cadherin在细胞膜上的表达增加，间质性标记因子在细胞质内的表达随之下降。HeLa-siE7细胞迁移及侵袭能力明显低于对照组（P<0.01）；沉默HPV18-E7表达的HeLa细胞其增殖能力也明显减弱。结论：宫颈腺癌细胞中HPV18-E7能够引起EMT发生，促进肿瘤发生侵袭转移。
Abstract:

Objective: To determine the role of HPV18-E7 oncogene in the EMT-like process. Methods: HPV18-E7 siRNAs were transfected in human cervical cancer HeLa cells. Real-time PCR, western blot and immunofluorescence technique were performed to examine the localization and expression of E7 andE MT markers, respectively. Furthermore, the Wound healing assay and Matrigel invasion assay were used to evaluate the invasion and migration ability. Results: On the mRNA and protein levels, E7 depletion significantly induced the expression of E-caderin. Furthermore, N-caderin, Vimetin, and fibronectin expression were decreased in HeLa-siE7 cells. E-cadherin was up-regulated and mainly observed in contact areas between cells in HeLa/siE7 cells, while the mesenchymal markers such as N-caderin, and fibronectin were reduced and detected mainly in the cytoplasm. Down-regulation of HPV18-E7 by transient transfection of HPV18-E7 siRNAs in HeLa cells caused significant inhibition of cell invasion and migration compared with the siNC transfected cells. Conclusion: In HeLa cells HPV18-E7 can enhance invasion and migration by inducing EMT.

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参考文献/References:

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备注/Memo

备注/Memo:

作者简介 米合日尼沙·买买提（1976-），女，副主任医师，硕士，研究方向：妇科肿

瘤的诊治 ；通信作者：韩莉莉，E-mail：hanliliabcd@163.com。

更新日期/Last Update: 2016-11-25