|本期目录/Table of Contents|

[1]李 冰,冯 凭.罗格列酮干预糖尿病大鼠脂肪组织CMKLR1及Chemerin基因表达[J].天津医科大学学报,2015,21(06):484-487.
 LI Bing,FENG Ping.Effect of rosiglitazone on the expression of CMKLR1 and Chemerin mRNA in the adipose tissue of diabetic rat[J].Journal of Tianjin Medical University,2015,21(06):484-487.
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罗格列酮干预糖尿病大鼠脂肪组织CMKLR1及Chemerin基因表达(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21
期数:
2015年06期
页码:
484-487
栏目:
基础医学
出版日期:
2015-11-20

文章信息/Info

Title:
Effect of rosiglitazone on the expression of CMKLR1 and Chemerin mRNA in the adipose tissue of diabetic rat
文章编号:
1006-8147(2015)06-0484-04
作者:
李 冰1冯 凭2
(1.天津市儿童医院ICU,天津300134;2.天津医科大学总医院代谢病科,天津300070)
Author(s):
?LI Bing1 FENG Ping2
(1.Department of Intensive Care Unit, Tianjin Children Hospital,Tianjin300074,China;2.Department of Metabolism,General Hospital,Tianjin Medical University,Tianjin 300070,China)
关键词:
罗格列酮' target="_blank" rel="external">">罗格列酮糖尿病大鼠Chemerin-CMKLR1基因表达
Keywords:
rosiglitazone diabetes rat Chemerin-CMKLR1 gene expression
分类号:
R587.1
DOI:
-
文献标志码:
A
摘要:
目的:通过检测罗格列酮干预后CMKLR1及Chemerin在自发性2型糖尿病大鼠脂肪组织中表达水平,探讨Chemerin-CMKLR1通路在肥胖、胰岛素抵抗及2型糖尿病发病机制中的作用。方法:4周龄糖尿病大鼠30只,经相关实验至30周证实造模成功2型糖尿病大鼠20只,再随机分为罗格列酮干预组和模型组各10只,进行相关实验室指标检测,以实时荧光定量法检测脂肪组织CMKLR1及Chemerin基因的表达水平。结果:罗格列酮干预后大鼠各实验室指标明显改善,干预组大鼠网膜组织CMKLR1的表达明显低于模型组(P <0.01),干预后网膜及皮下脂肪组织中Chemerin的表达明显低于模型组(P <0.01)。罗格列酮干预后网膜脂肪细胞平均最大直径明显缩小(P<0.01),而皮下脂肪细胞平均最大直径无明显变化(P >0.05)。结论:Chemerin-CMKLR1通路成为治疗肥胖、胰岛素抵抗和2型糖尿病的新靶点。
Abstract:

Objective: To investigate the expression of CMKLR1 and Chemerin mRNA in subcutaneous and visceral adipose tissue of rats and discuss the intervention of Chemerin-CMKLR1 to involve in the pathogenesis of obesity, insulin resistance and type 2 diabetes. Methods: Thirty male rats of 4 weeks were selected. Twenty rats were proved with diabetes through experimental methods after 30 weeks. Models were separated into rosiglitazone group and diabetes group with ten rats in each group. Experiment dates were detected. The mRNA levels of CMKLR1 and Chemerin in adipose tissue by real-time PCR. Results: Experiments dates were improved after the intervention of rosiglitazone. The mRNA levels of CMKLR1 in visceral adipose tissue of rats in rosiglitazone group were lower than those in diabetes group(P<0.01).The mRNA levels of Chemerin of subcutaneous and visceral adipose tissue of rats in rosiglitazone group were lower than those in diabetes group (P<0.01). Maximum diameter in visceral adipocyte in rosiglitazone group decreased compared with that in diabetes group (p<0.01). Maximum diameter was of no difference in subcutaneous tissue between two groups (P >0.05). Conclusion: The intervention of the expression of CMKLR1 and Chemerin mRNA become new treatment target of obesity and insulin resistance and type 2 diabetes by rosiglitazone.

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参考文献/References:

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备注/Memo

备注/Memo:

作者简介 李冰(1983-),女,医师,硕士,研究方向:儿内科学;通信作者:冯凭,E-mail:duaiyong@sina.cn。



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更新日期/Last Update: 2015-11-27