|本期目录/Table of Contents|

[1]刘超,韩雅婷,杨 冰,等.基础医学模式生物酿酒酵母接合型的鉴定—PCR法[J].天津医科大学学报,2016,22(03):256-258.
　LIU Chao,HAN Ya-ting,YANG Bing,et al.Identification of the mating-type of model organism S. cerevisiae in basic medical science by PCR[J].Journal of Tianjin Medical University,2016,22(03):256-258.
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基础医学模式生物酿酒酵母接合型的鉴定—PCR法(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
22卷
期数:
2016年03期
页码:
256-258
栏目:
技术与方法
出版日期:
2016-05-20

文章信息/Info

Title:
Identification of the mating-type of model organism S. cerevisiae in basic medical science by PCR
文章编号:
1006-8147（2016）03-0256-03
作者:
刘超; 韩雅婷; 杨 冰; 孙 琰; 王 玺
（天津医科大学基础医学院细胞生物学系天津 300070 ）
Author(s):
?LIU Chao; HAN Ya-ting; YANG Bing; SUN Yan; WANG Xi
(Department of Cell Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China)
关键词:
酿酒酵母' target="_blank" rel="external">">酿酒酵母; 接合型; MAT基因座; PCR鉴定; 单菌落PCR
Keywords:
S. cerevisiae;  mating-type;  MAT locus;  PCR identification;  colony PCR
分类号:
Q2
DOI:
-
文献标志码:
A
摘要:
目的：利用PCR法鉴定酿酒酵母a、α单倍体或a/α双倍体三种接合型。 方法：根据酿酒酵母a、α或a/α三种接合型MAT基因座及附近序列信息，分别设计特异的寡核苷酸引物。提取酿酒酵母DNA作为模版和利用单菌落PCR法，进行特异性PCR扩增。 结果：MATa基因座特异性引物能在a和a/α接合型中扩增出特异性条带；MATα基因座特异性引物则能在α和a/α接合型中扩增出特异性条带。 结论：利用PCR鉴定酿酒酵母接合型是一种操作简单，准确灵敏的方法，为基础医学单细胞真核模式生物的研究提供方便。
Abstract:
?Objective: To identify S. Cerevisiae mating-type to be be a, α haploid or a/α diploid by PCR. Methods: Specific PCR primers were designed according to the MAT locus and its nearby sequence of the three mating-types, and applied in specific PCR reaction using genome DNA as template or the method of colony PCR. Results: A specific band was obtained in a or a/α mating-type when specific primers were used on MATa, while a specific band was obtained in α or a/α mating-type by using specific primers on MATα. Conclusion: With specific primers, PCR may be a convenient, fast and precise method to identify S. cerevisiae mating-type, which could facilitate the study of monocellular eukaryotes model organism in basic medical science.

参考文献/References:

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备注/Memo

备注/Memo:

?作者简介 刘超（1990—），男，硕士在读，研究方向：肿瘤表观遗传学；通信作者：王玺，E-mail：wangxi@tijmu.edu.cn。



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更新日期/Last Update: 2016-05-25