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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

19卷

期数:

2013年06期

页码:

437-440

栏目:

基础医学

出版日期:

2013-11-20

文章信息/Info

Title:

Experimental study of AURKA protein kinase in the formation of vascular mimicry in triple-negative breast cancer stem cells

作者:

刘 营¹; 孙保存 ¹; 2; 刘铁菊¹; 赵秀兰¹; 李岩磊¹

1.天津医科大学病理学教研室，天津300070；2.天津医科大学附属肿瘤医院病理科，天津300060

Author(s):

LIU Ying¹;  SUN Bao-cun¹;  ²;  LIU Tie-ju¹;  ZHAO Xiu-lan¹;  LI Yan-lei¹

1.Department of Pathology, Tianjin Medical University, Tianjin 300070, China; 2.Department of Pathology, Cancer Hospital, Tianjin Medical University, Tianjin 300060, China

关键词:

AURKA; 乳腺癌; 干细胞; 血管拟态

Keywords:

-

分类号:

R737.9

DOI:

-

文献标志码:

A

摘要:

目的：探讨AURKA蛋白激酶在三阴乳腺癌干细胞形成血管拟态中的作用。方法：用无血清悬浮培养法从三阴乳腺癌细胞MDA-MB-231分离获得干细胞，RT-PCR检测干细胞转录因子c-myc、sox-2的表达状态；三维培养观察干细胞形成血管能力；Western blot 检测AURKA蛋白激酶在干细胞和常规培养细胞的表达；利用干细胞进行三维培养并加入AURKA蛋白激酶抑制剂观察对成血管能力的影响。结果：（1）利用干细胞无血清悬浮培养可获得MDA-MB-231干细胞，其干细胞转录因子c-myc、sox-2表达高于常规培养细胞。（2）AURKA蛋白激酶在乳腺癌干细胞球中较常规培养细胞表达升高。（3）干细胞三维培养可形成血管管道，加入AURKA蛋白激酶抑制剂后成血管能力减弱。结论：AURKA蛋白激酶可促进三阴乳腺癌干细胞形成血管拟态，抑制其表达能减弱形成血管拟态能力。

Abstract:

Objective: To investigate the role of AURKA protein kinase in the formation of vascular mimicry in triple-negative breast cancer stem cells. Methods: The serum-free suspension culture of breast spheres from triple-negative breast cancer cells MDA-MB-231 cells were isolated, the expression of stem cell transcription factor status of c-myc and sox-2 were detected by RT-PCR. The formation of vascular stem cells were observed by three-dimensional culture. The expression of AURKA protein kinase from stem and normal cells were detected by western blot. Three-dimensional culture and AURKA protein kinase inhibitor were used to detect the formation ability of blood vessels. Results: (1) The expression of stem cell transcription factor status of c-myc and sox-2 were highter than conventional cell culture in MDA-MB-231 stem cells obtained from the serum-free suspension culture. (2) The expression of AURKA protein kinase were higher than conventional cell culture in MDA-MB-231 stem cells obtained from the serum-free suspension culture.(3) Stem cells contributed to the formation of vascular pipeline, AURKA protein kinase inhibitor diminished the formation of vascular. Conclusion: AURKA plays a key role in the formation of vascular mimicry in triple-negative breast cancer stem cells，inhibiting its expression and weakening the ability of the formation of vascular mimicry.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金重点项目（81230050）；天津市科委项目（125JCYBJC15500）作者简介 刘营（1986-），男，硕士在读，研究方向：肿瘤血管生成；通信作者：孙保存，E-mail:sunbaocun@aliyun.com。

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更新日期/Last Update: 2013-12-20