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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年06期

页码:

421-425

栏目:

基础医学

出版日期:

2014-11-20

文章信息/Info

Title:

Up-regulation of Nanog promotes cell proliferation and invasion in breast cancer cells MCF-7

文章编号:

1006-8147（2014）06-0421-05

作者:

孙秀梅; 张 飞; 田 然; 刘 媛; 王智勇; 杨 毅; 魏熙胤; 牛瑞芳

(天津医科大学肿瘤医院公共实验室,国家肿瘤临床医学研究中心,天津市“肿瘤防治”重点实验室,乳腺癌防治教育部重点实验室,天津 300060)

Author(s):

SUN Xiu-mei;  ZHANG Fei;  TIAN Ran;  LIU Yuan;  WANG Zhi-yong;  YANG Yi;  WEI Xi-yin;  NIU Rui-fang

(Department of Public Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin 300060,China)

关键词:

Nanog; 增殖; 迁移; 侵袭; MCF-7; 乳腺癌

Keywords:

Nanog; proliferation; migration; invasion; MCF-7; breast cancer

分类号:

R737.9

DOI:

-

文献标志码:

A

摘要:

目的：观察Nanog高表达对乳腺癌细胞MCF-7生物学特性的影响。方法：构建重组质粒pcDNA3.1(-)-Nanog，利用免疫荧光观察Nanog高表达后，乳腺癌细胞MCF-7中Nanog的定位。利用平皿克隆分离法获得Nanog高表达的乳腺癌细胞系MCF-7。应用克隆形成实验、Transwell迁移和侵袭实验观察Nanog高表达对乳腺癌细胞MCF-7生物学特性的影响。结果：Nanog在MCF-7细胞中定位于核，Nanog高表达后，乳腺癌细胞MCF-7的增殖、迁移和侵袭能力显著增强。结论：Nanog能够促进乳腺癌细胞MCF-7的增殖、迁移和侵袭能力。

Abstract:

Objective: To observe the effects of up-regulation of Nanog on the biological characteristics of breast cancer cells MCF-7. Methods: A recombinant plasmid pcDNA3.1(-)-Nanog was constructed. Using immunofluorescence, Nanog positioning in MCF-7 was observed after transfected with plasmid pcDNA3.1(-)-Nanog. Breast cancer cell line MCF-7 high expression Nanog was obtained by using cloning separation plate. The effects of up-regulation of Nanog on the biological characteristics of MCF-7 breast cancer cells were observed by clonogenic assay, Transwell migration and invasion assay. Results: Nanog was localized in the nucleus in MCF-7 cells. MCF-7 high expression of Nanog had more significant effect on cell proliferation, migration and invasion. Conclusion: Up-regulation of Nanog could promote cell proliferation, migration and invasion of MCF-7

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81372844： 81071731）

作者简介 孙秀梅（1985-），女，硕士在读，研究方向：生物化学与分子生物学；通信作者：牛瑞芳，E-mail:niuruifang@tjmuch.com。

 

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更新日期/Last Update: 2014-12-03