|本期目录/Table of Contents|

[1]李丽敏,孙卫星.TRIM22 抑制PDGF-BB诱导的气道平滑肌细胞的炎症、增殖和迁移[J].天津医科大学学报,2025,31(04):358-363.[doi:10.20135/j.issn.1006-8147.2025.04.0358]
　LI Limin,SUN Weixing.TRIM22 inhibits inflammation， proliferation， and migration of PDGF-induced airway smooth muscle cells[J].Journal of Tianjin Medical University,2025,31(04):358-363.[doi:10.20135/j.issn.1006-8147.2025.04.0358]
点击复制

TRIM22 抑制PDGF-BB诱导的气道平滑肌细胞的炎症、增殖和迁移(PDF)

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
31卷
期数:
2025年04期
页码:
358-363
栏目:
基础医学
出版日期:
2025-07-10

文章信息/Info

Title:
TRIM22 inhibits inflammation， proliferation， and migration of PDGF-induced airway smooth muscle cells
文章编号:
1006-8147(2025)04-0358-06
作者:
李丽敏1; 孙卫星2
（义马煤业集团股份有限公司总医院1.呼吸与危重症医学科；2.儿科，义马 472300）
Author(s):
LI Limin1;  SUN Weixing2
（1.Department of Respiratory and Critical Care Medicine，Yima Coal Industry Group Company General Hospital，Yima 472300，China；2.Department of Pediatrics，Yima Coal Industry Group Company General Hospital，Yima 472300，China）
关键词:
哮喘; TRIM22; PDGF-BB; 气道平滑肌细胞; PI3K/Akt
Keywords:
asthma;  TRIM22;  PDGF-BB;  airway smooth muscle cells;  PI3K/Akt
分类号:
R56
DOI:
10.20135/j.issn.1006-8147.2025.04.0358
文献标志码:
A
摘要:
目的：探讨 TRIM22 在气道平滑肌细胞（ASMCs）功能中的作用。方法：以血小板衍生生长因子（PDGF）-BB诱导的ASMCs为哮喘细胞模型，采用qPCR和免疫印迹检测TRIM22表达，利用CCK-8、Transwell及伤口愈合实验评估细胞增殖与迁移能力，采用ELISA和免疫印迹法检测炎症，并通过免疫印迹验证其作用机制。结果：TRIM22在PDGF-BB诱导的ASMCs中表达水平降低（t=19.57，P<0.001）；PDGF-BB处理组ASMCs的细胞活力显著提高（P<0.01），而TRIM22过表达显著抑制了PDGF-BB引起的细胞生长能力增强（t=7.93，P<0.01），同时显著抑制PDGF-BB引起的迁移能力提升（t=7.95，P<0.001）。TRIM22过表达能够显著抑制PDGF-BB诱导的炎症因子[肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、IL-6]分泌（TNF-α：t=5.57，P<0.01；IL-1β：t=5.53，P<0.01；IL-6：t=7.42，P<0.01）。此外，免疫印迹实验结果表明，PDGF-BB诱导的ASMCs中核因子（NF）-κB的磷酸化水平显著升高，而TRIM22过表达显著降低了NF-κB的磷酸化水平（t=9.51，P<0.01）。同时，TRIM22抑制了PDGF-BB诱导的ASMCs中磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（Akt）信号通路的激活，表现为抑制PDGF-BB诱导的PI3K和Akt的磷酸化水平（PI3K：t=5.61，P<0.01；Akt：t=3.56，P<0.01）。结论：TRIM22通过PI3K/Akt信号通路抑制PDGF-BB引起的ASMCs的炎症、增殖及迁移。
Abstract:
Objective： To investigate the role of TRIM22 in the function of airway smooth muscle cells（ASMCs）. Methods： Platelet-derived growth factor （PDGF）-BB induced ASMCs were used as a cellular model of asthma. The expression of TRIM22 was detected using qPCR and immunoblotting. Cell proliferation and migration were assessed using CCK-8， Transwell， and wound-healing assays. Inflammatory responses were evaluated by ELISA and Western blotting. The mechanism of action was further validated through Western blotting. Results： The expression level of TRIM22 in PDGF-BB was induced ASMCs decreased （t=19.57，P<0.001）. In the PDGF-BB treatment group， the cell viability of ASMCs was significantly increased （P<0.01）， while the overexpression of TRIM22 significantly inhibited the enhancement of cell growth induced by PDGF-BB（t=7.93，P<0.01） and the enhancement of migration induced by PDGF-BB （t=7.95，P<0.001）. Overexpression of TRIM22 could significantly inhibit the secretion of PDGF-BB induced inflammatory cytokines including tumor necrosis factor （TNF）-α， interleukin （IL）-1 β， and IL-6 （TNF-α：t=5.57，P<0.01；IL-1β：t=5.53，P<0.01；IL-6：t=7.42，P<0.01）. In addition， Western blotting showed that the phosphorylation level of nuclear factor （NF）-κB in ASMCs induced by PDGF-BB was significantly increased， while overexpression of TRIM22 significantly decreased the phosphorylation level of NF-κB（t=9.51，P<0.01）. Meanwhile， TRIM22 inhibited the activation of phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） signaling pathway in ASMCs induced by PDGF-BB， and inhibited the phosphorylation levels of PI3K and Akt induced by PDGF-BB（PI3K：t=5.61，P<0.01； Akt：t=3.56，P<0.01）. Conclusion： TRIM22 suppresses inflammation， proliferation， and migration of PDGF-BB induced ASMCs through the PI3K/Akt pathway.

参考文献/References:

[1] TREMBLEY J H， BARACH P， TOM?譧?譒KA J M， et al. Current understanding of the impact of United States military airborne hazards and burn pit exposures on respiratory health[J]. Part Fibre Toxicol， 2024，21（1）：43.
[2] DIN K M， ALAM F， SARFRAZ M， et al. Pharmacognostic characterization of primulamacrophylla by light microscopy， scanning electron microscopy and analytical techniques[J]. Microsc Res Tech， 2025， 88（4）：1087-1101.
[3] HOSSEINI Z， GHOLAMI M， BONYADI Z. Impact of climate change on human health [J]. Iran Biomed J， 2024， 28（7）： 78.
[4] CHEN G， ZHENG Y， WU N， et al. Human beta defensin 3 knockdown inhibits the proliferation and migration of airway smooth muscle cells through regulating the PI3K/AKT signaling pathway[J]. Mol Immunol， 2024，168：38-46.
[5] LU J， WANG M， ZHOU Y， et al. Tripartite motif 22 interacts with protein phosphatase magnesium-dependent 1 A to aggravate radiation-induced epithelial-mesenchymal transition and fibrogenesis in lung epithelial cells [J]. Toxicol In Vitro， 2025， 103： 105972.
[6] ZHANG N， ZHANG H， BAO X， et al. Transactivation DNA-binding protein-related genes were associated with salivary gland injury in primary Sj?觟gren syndrome[J]. Medicine， 2024， 103（39）： e39827.
[7] YANG Y， HAO X， ZHANG J， et al. The E3 ligase TRIM22 functions as a tumor suppressor in breast cancer by targeting CCS for proteasomal degradation to inhibit STAT3 signaling[J]. Cancer Lett， 2024， 600： 217157.
[8] OGAWA M， KITAMOTO J， TAKEDA T， et al. Bisphenol A prevents MCF-7 breast cell apoptosis via the inhibition of progesterone receptor transactivation[J]. J Biochem Mol Toxicol， 2023， 37（7）： e23367.
[9] WEI Y， HUANG X， MA Y， et al. FOXC1-mediated TRIM22 regulates the excessive proliferation and inflammation of fibroblast-like synoviocytes in rheumatoid arthritis via NF-κB signaling pathway [J]. Mol Med Rep， 2022， 26（4）： 304.
[10] SINGH P， KUMARI S， CHAKRAVORTTY H， et al. In vivo， in vitro， and in silico approaches in the detailed study of di-butyl phthalate （DBP）， a plasticizer-induced lung fibrosis via Nrf-2/Keap-1/HO-1 pathway and its regulation[J]. Bioorg Chem， 2024， 154： 107970.
[11] LIU Y， LI J， CHEN R， et al. Airway epithelial cells promote in vitro airway smooth muscle cell proliferation by activating the Wnt/β-catenin pathway [J]. Respir Physiol Neurobiol， 2024， 331： 104368.
[12] AMBHORE N S， BALRAJ P， KUMAR A， et al. Kiss1 receptor knockout exacerbates airway hyperresponsiveness and remodeling in a mouse model of allergic asthma [J]. Respir Res， 2024， 25（1）： 387.
[13] NI K， CHE B， GU R， et al. Single-cell hypertrophy promotes contractile function of cultured human airway smooth muscle cells via piezo1 and YAP auto-regulation [J]. Cells， 2024， 13（20）： 1697.
[14] LIU Y， YIN Q， LIU B， et al. Fisetin reduces ovalbumin-triggered airway remodeling by preventing phenotypic switching of airway smooth muscle cells [J]. Respir Res， 2024， 25（1）： 370.
[15] LI S， QU L， ZHOU L， et al. Biomass fuels related-PM（2.5） promotes lung fibroblast-myofibroblast transition through PI3K/AKT/TRPC1 pathway [J]. Ecotoxicol Environ Saf， 2024， 276： 116309.

相似文献/References:

[1]马利锋,刘长山,王雪艳,等.呼出气一氧化氮测定在儿童哮喘控制评估中的价值[J].天津医科大学学报,2013,19(05):407.
[2]杨晓丽,蒋 萍.超重对哮喘患者肺功能及呼出气一氧化氮的影响[J].天津医科大学学报,2013,19(06):493.
　YANG Xiao-li,JIANG Ping.Effect of overweight on lung function and fractional exhaled nitricoxide of asthma patients[J].Journal of Tianjin Medical University,2013,19(04):493.
[3]周秋梅,刘长山.中短程低剂量吸入糖皮质激素对哮喘儿童生长的影响[J].天津医科大学学报,2016,22(04):322.
　ZHOU Qiu-mei,LIU Chang-shan.Efficacy of short-medium term and low dose inhalation of corticosteroids in treatment of asthma and its effect on growth in children[J].Journal of Tianjin Medical University,2016,22(04):322.
[4]刘梦昭,刘长山.粉尘螨滴剂舌下免疫治疗哮喘伴过敏性鼻炎患儿的临床疗效及安全性[J].天津医科大学学报,2018,24(04):344.
　LIU Meng-zhao,LIU Chang-shan.Efficacy and safety of sublingual immunotherapy with dermatophagoides farinae drops for children with allergic rhinitis and asthma[J].Journal of Tianjin Medical University,2018,24(04):344.
[5]熊蕾蕾,张松林,张向峰,等.R848对哮喘患儿外周血中IFN-γ、IL-33和IgE表达的影响及临床意义[J].天津医科大学学报,2021,27(06):623.
　XIONG Lei-lei,ZHANG Song-lin,ZHANG Xiang-feng,et al.The effect of R848 on the expression of IFN-γ, IL-33 and IgE in the peripheral blood of children with asthma and its clinical significance[J].Journal of Tianjin Medical University,2021,27(04):623.
[6]李昱剑,阚璇.哮喘中miRNA、lncRNA、circRNA、转录因子和靶基因的调控网络[J].天津医科大学学报,2023,29(03):235.
　LI Yu-jian,KAN Xuan.Regulatory networks of miRNA，lncRNA，circRNA，transcription factors and target genes in asthma[J].Journal of Tianjin Medical University,2023,29(04):235.
[7]茹仙古丽·吾买尔,索睿 综述,赵晓赟 审校.数字疗法在哮喘疾病管理中的应用[J].天津医科大学学报,2023,29(03):344.
[8]索睿,郝天旭,茹仙古丽·吾买尔 综述,等.间充质干细胞来源的外泌体治疗哮喘的研究进展[J].天津医科大学学报,2023,29(06):673.

备注/Memo

备注/Memo:
作者简介 李丽敏（1981-），女，学士，副主任医师，研究方向：呼吸与危重症；通信作者：孙卫星，E-mail：15239886278@163.com。
更新日期/Last Update: 2025-07-10