|本期目录/Table of Contents|

[1]徐晓晴,邱帅,陈喜娟,等.胆囊收缩素对小肠I细胞及胰腺细胞和小鼠胰腺消化酶储备的影响[J].天津医科大学学报,2022,28(02):156-159.
　XU Xiao-qing,QIU Shuai,CHEN Xi-juan,et al.Effects of cholecystokinin on the intestinal I cells，pancreatic cells and pancreatic digestive enzyme reserve in mice[J].Journal of Tianjin Medical University,2022,28(02):156-159.
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胆囊收缩素对小肠I细胞及胰腺细胞和小鼠胰腺消化酶储备的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
28卷
期数:
2022年02期
页码:
156-159
栏目:
基础医学
出版日期:
2022-03-20

文章信息/Info

Title:
Effects of cholecystokinin on the intestinal I cells，pancreatic cells and pancreatic digestive enzyme reserve in mice
文章编号:
1006-8147(2022)02-0156-04
作者:
徐晓晴1; 邱帅1; 陈喜娟1; 胡立娟2; 李秋菊1; 张大鹏2; 王丰2
（1.天津医科大学研究生院，天津 300070；2.天津市南开医院中西医结合急腹症研究所，天津 300100）
Author(s):
XU Xiao-qing1; QIU Shuai1; CHEN Xi-juan1; HU Li-juan2; LI Qiu-ju1; ZHANG Da-peng2;  WANG Feng2
（1.Graduate School，Tianjin Medical University，Tianjin 300070，China；2. The Laboratory of Acute Abdomen Disease Associated Organ Injury and Repair，Nankai Hospital Affiliated to Nankai University，Tianjin 300100，China）
关键词:
胰胆转流术; 小鼠; 胰腺; 胆囊收缩素; 分泌; 酶学; 细胞增殖; I细胞
Keywords:
pancreatobiliary diversion;  mouse;  pancreas; cholecystokinin; secretions; enzymology; cell proliferation; I cells
分类号:
R57
DOI:
-
文献标志码:
A
摘要:
目的：研究胰胆转流（PBD）术造成的高胆囊收缩素（CCK）血症对小鼠小肠I细胞群落及胰腺细胞和胰腺消化酶含量的影响。方法： 40只雄性 Balb/C 裸鼠随机分为PBD组及假手术（SO）组，每组20只，分别实施PBD术和假手术。20 d后称重胰腺重量，并用酶联免疫吸附法测定血浆CCK，用免疫组化法在十二指肠中测定I细胞群落，在胰腺中检测CCK受体和增殖细胞核抗原，以酶联免疫吸附法测定胰腺组织中胰蛋白酶原、脂肪酶、淀粉酶的含量。结果：PBD组血浆CCK水平高于SO组 （t=16.59，P＜0.01），胰腺重量也较SO组增加（t=2.51，P＜0.05）；且PBD组十二指肠内的I细胞多于SO组 （t=5.04，P＜0.01）。进一步的胰腺检测显示，PBD组CCK受体表达增强（t=5.08，P＜0.01）、增殖细胞核抗原增多（t=8.05，P＜0.01）；比较两组胰腺外分泌酶的组成，PBD组胰腺中的胰蛋白酶原 （t=4.31，P<0.01）、脂肪酶（t=2.15，P<0.05）的浓度增加；胰淀粉酶浓度虽然较SO组有增加的趋势，但差异无统计学意义（t=0.60，P=0.56）；PBD组全胰蛋白酶含量（t=2.49，P<0.05）、脂肪酶含量（t=2.02，P<0.05）、淀粉酶含量（t=2.53，P<0.05） 均较SO组增高。结论：CCK可刺激胰腺发育，使小肠I细胞增生，选择性地增加胰腺中的胰蛋白酶原和胰脂酶储备。
Abstract:
Objective：To study the effects of hypercholecystokin（CCK） inemia induced by pancreaticobiliary（PBD） on the proliferation of small intestinal I cell and pancreatic cells and the composition of pancreatic exocrine enzymes in mice. Methods：Forty male mice were randomly divided into PBD group and sham operation（SO） group，with 20 in each group. After 20 days，the weight of pancreas was weighed，

参考文献/References:

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金（81572318）
作者简介 徐晓晴（1994-），女，硕士在读，研究方向：胰腺癌基础研究；通信作者：王丰，E-mail：fengwangpi@163.com。
更新日期/Last Update: 2022-03-20