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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年05期

页码:

354-356

栏目:

基础医学

出版日期:

2014-09-20

文章信息/Info

Title:

Effect of Nrf2 in hydrogen-rich saline to reduce the oxidative damage in septic mice

文章编号:

1006-8147(2014)05-0354-03

作者:

高秋文; 谢克亮; 陈红光; 王卫娜; 王国林; 于泳浩

（天津医科大学总医院麻醉科，天津 300052）

Author(s):

GAO Qiu-wen;  XIE Ke-liang;  CHEN Hong-guang;  WANG Wei-na;  WANG Guo-lin;  YU Yong-hao

(Department of Anesthesiology, General Hospital, Tianjin Medical University, Tianjin 300052, China)

关键词:

富氢液; 脓毒症; 氧化损伤; 小鼠; 核因子E2相关因子2

Keywords:

hydrogen-rich saline;  sepsis;  oxidative injury;  mice;  Nrf2

分类号:

R631

DOI:

-

文献标志码:

A

摘要:

目的： 探讨富氢液对小鼠脓毒症氧化损伤的影响:及核因子E2相关因子 2（Nrf2）的具体作用机制。方法：雄性C57BL/6野生型小鼠只，体20～25 g，5周龄，采用随机数字表法，将其随机分为4组(n=16)：假手术组（A组）、盲肠结扎穿孔（CLP）组（B组）、CLP＋富氢液组（C组）、CLP＋富氢液+全反式维甲酸（ATRA）组（D组）。采用CLP制备脓毒症模型。ATRA处理为CLP实验前1周，每天腹腔注射ATRA，10 mg/kg。各组于CLP或假手术后1、6 h时分别给予富氢液（HS）或生理盐水，5 mL/kg。每组取10只小鼠，观察CLP后7 d内生存情况。剩余24只动物，于手术后24 h下腔静脉采血，检测血清丙二醛（MDA）、抑制羟自由基（·OH）能力、超氧化物酶（SOD）和过氧化氢酶（CAT）水平，取肺检测Nrf2的表达。结果: 与A组比较，B组小鼠7d生存率降低，肺组织Nrf2表达增加，血清MDA含量增加，抑制·OH能力、SOD和CAT活性降低（P < 0.05）；与B组比较，C组小鼠7 d生存率升高（P < 0.05），肺组织Nrf2表达进一步增加，血清MDA含量降低，抑制·OH能力、SOD和CAT活性增加（P < 0.05）；与C组比较，D组小鼠7 d生存率显著下降（P < 0.05），肺组织Nrf2表达降低，血清MDA含量增加，抑制·OH能力、SOD和CAT活性降低（P < 0.05）。结论：富氢液对脓毒症氧化损伤具有治疗作用，可能与调节内源性抗氧化系统关键分子Nrf2有关。

Abstract:

 Objective: To investigate the effects of hydrogen-rich saline on sepsis in mice and the associated mechanism. Methods: Sixty four mice aged 5 weeks weighing 20-25 g were randomly divided into four groups (n=16 each): sham operation group (group A), CLP group (group B) , CLP + hydrogen-rich saline group (group C), CLP + hydrogen-rich saline + ATRA group (group D). Severe sepsis was induced by cecal ligation and puncture (CLP) operation. ATRA was given i.p. daily at 10mg/kg for 1 week before CLP. 5ml/kg of hydrogen-rich saline was given for 1 h beginning at 1 and 6 h after CLP or sham operation, respectively. The survival rates of four groups (40 animals) were observed in 7 day. In additional 24 animals, blood was collected to test malondialdehyde (MDA), inhibited ability of hydroxyl radical, activity of superoxide dismutase (SOD) and catalase (CAT) at 24 h after operation, then lung were collected to measure the Nrf2 protein expression. Results: Compared with group A, survival rate of severe septic mice, the inhibited ability of hydroxyl radical, the activity of SOD and CAT were significantly decreased and the MDA was increased, the Nrf2 expression was increased in group B (P < 0.05). Compared with group B, survival rate of severe septic mice, the inhibited ability of hydroxyl radical, SOD and CAT activities were significantly elevated and the MDA was decreased in serum of septic mice, the Nrf2 expression was further increased in group C (P < 0.05). Compared with group C, survival rate of severe septic mice, the inhibited ability of hydroxyl radical, the activity of SOD and CAT were obviously decreased and the MDA was increased, the Nrf2 expression was reduced in group D (P < 0.05). Conclusion: Hydrogen-rich saline can alleviate sever sepsis in mice via regulating Nrf2 protein expression. 
 

参考文献/References:

[1] Cheng B, Xie G, Yao S, et al. Epidemiology of severe sepsis in critically ill surgical patients in ten university hospitals in China. Crit Care Med，2007,35(11): 2538
[2] Reid V L, Webster N R. Role of microparticles in sepsis. Br J Anaesth，2012，109(4):503
[3] Xie K, Yu Y, Pei Y, et al. Protective ffects of hydrogen gas on murine polymicrobial sepsis via reducing oxidative stress and HMGB1 release[J]. Shock，2010, 34: 90
[4] Li G M, Ji M H, Sun X J, et al. Effects of hydrogen-rich saline treatment on polymicrobial sepsis. J Surg Res， 181(2) :279
[5] 陈红光，谢克亮，韩焕芝，等氢气对脓毒症小鼠急性肺损伤的影响。中华麻醉学杂志，2012，32（2）：195
[6] Thimmulappa R K, Lee H, Rangasamy T,et al.Nrf2 is a critical regulator of the innate immune response and survival during experimental sepsis. J Clin Invest，2006, 116(4):984
[7] Wang X J, Hayes J D, Henderson C J,et al. Identification of retinoic acid as an inhibitor of transcription factor Nrf2 through activation of retinoic acid receptor alpha. PNAS，2007, (49):19589

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备注/Memo

备注/Memo:

(基金项目 国家自然科学基金资助项目（81071533, 81101409）；天津市应用基础及前沿技术研究计划资助（11JCYBJC12900, 13JCQNJC11400）；天津市卫生局资助项目（2011KZ108）作者简介 高秋文（1968-），女，本科，研究方向：麻醉护理；通信作者：于泳浩，Email: yuyonghao@126.com。

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更新日期/Last Update: 2014-09-25