«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

27卷

期数:

2021年06期

页码:

595-602

栏目:

基础医学

出版日期:

2021-11-15

文章信息/Info

Title:

Based on network pharmacology，molecular docking，and experiments to explore the mechanism of Yinchenhao Decoction in regulating oxidative stress of obstructive jaundice

文章编号:

1006-8147（2021）06-0595-08

作者:

陈帅¹; 刘军舰²; 尚海涛²; 张井虹¹; 李忠廉²

（1.天津医科大学研究生院，天津 300070；2.天津市中西医结合医院肝胆胰第二外科，天津 300100）

Author(s):

CHEN Shuai¹; LIU Jun-jian²; SHANG Hai-tao²; ZHANG Jing-hong¹; LI Zhong-lian²

（1.Graduate School of Tianjin Medical University，Tianjin 300070，China;2.Department of The Second Hepatobiliary and Pancreatic Surgery，Tianjin Hospital of Integrated Traditional Chinese and Western Medicine，Tianjin 300100，China）

关键词:

茵陈蒿汤; 阻塞性黄疸; 网络药理学; 分子对接; eNOS; iNOS

Keywords:

Yinchenhao Decoction; obstructive jaundice; network pharmacology; molecular docking; eNOS; iNOS

分类号:

R285

DOI:

-

文献标志码:

A

摘要:

目的：运用网络药理学探讨茵陈蒿汤调节阻塞性黄疸氧化应激的作用机制，并用分子对接及免疫组化进行验证。方法：首先利用中药系统药理学数据库和分析平台（TCMSP）和UniProt数据库检索并筛选茵陈蒿汤有效成分及作用靶点；通过Gene Cards数据库检索阻塞性黄疸的作用靶点；利用R语言将疾病与药物的有效靶点进行分析，确定交集靶点；通过STRING数据库和Cytoscape软件构建蛋白互作网络；利用R语言进行基因本体论（GO）生物学过程富集分析和京都基因与基因组百科全书（KEGG）通路富集分析；利用Autodock_vina软件对内皮型一氧化氮合成酶（eNOS）、诱导型一氧化氮合成酶（iNOS）和茵陈蒿汤主要化学成分进行分子对接验证；最后通过免疫组织化学方法（IHC）验证茵陈蒿汤对阻塞性黄疸大鼠肝组织中eNOS和iNOS的影响。结果：研究共筛选获得茵陈蒿汤中29个有效成分及177个作用靶点，阻塞性黄疸的2 183个疾病靶点，茵陈蒿汤和阻塞性黄疸的交集靶点123个；经GO和KEGG富集分析得到133个GO生物过程和127个相关信号通路，主要涉及Hepatitis B、流体剪应力与动脉粥样硬化、丝裂原活化蛋白激酶（MAPK）、磷脂酰肌醇3激酶（PI3K）-蛋白激酶B（Akt）、肝细胞癌、低氧诱导因子（HIF）-1等信号通路；拓扑属性分析发现槲皮素、山奈酚、异鼠李素等主要成分和Akt1、TP53、白细胞介素（IL）6、血管内皮生长因子（VEGF）A、eNOS和iNOS等关键靶点。分子对接验证表明，eNOS和iNOS蛋白与槲皮素、山奈酚、异鼠李素有较好的亲和能力；IHC实验证明与假手术组相比，模型组肝组织中eNOS表达水平降低，而iNOS表达水平升高（P＜0.05）；与模型组相比，茵陈蒿汤组肝组织中eNOS表达水平升高，iNOS表达水平降低（P＜0.01）。结论：茵陈蒿汤对阻塞性黄疸的作用具有多成分、多靶点、多通路的特点，茵陈蒿汤对阻塞性黄疸氧化应激的调节可能与改变eNOS、iNOS蛋白的表达有关。

Abstract:

Objective: To explore the mechanism of Yinchenhao Decoction in regulating oxidative stress of obstructive jaundice by network pharmacology，and to verify it by molecular docking and immunohistochemistry. Methods: TCMSP and UniProt database were used to search and screen the effective components and targets of Yinchenhao Decoction. Gene Cards database was used to search the targets of obstructive jaundice. R language was used to analyze the effective targets of diseases and drugs to determine the intersection targets. STRING database and Cytoscape software were used to construct Protein Interaction network. R language was used to analyze the biological process of Gene Ontology（GO） and Kyoto Encyclopedia of Genes and Genomes（KEGG） pathway enrichment analysis. Autodock_Vina software was used to verify the molecular docking of endothelial nitric oxide synthase（eNOS），inducible nitric oxide synthase（iNOS），and main chemical components in Yinchenhao Decoction. Finally，the effect of Yinchenhao Decoction on eNOS and iNOS in liver tissue of rats with obstructive jaundice was verified by immunohistochemistry（IHC）. Results: A total of 29 active components and 177 targets in Yinchenhao Decoction，2 183 disease targets in obstructive jaundice and 123 intersection targets in Yinchenhao Decoction and obstructive jaundice were screened. Through GO and KEGG enrichment analysis，133 GO biological processes and 127 related signaling pathways were obtained，mainly involving the signaling pathways of hepatotis B，fluid shear stress and atherosclerosis，MAPK，PI3K-Akt，hepatocellular carcinoma，HIF-1 and so on. The main components of the content of Quercetin，Kaempferol and Isorhamnetin and the key targets of Akt1，TP53，IL6，VEGFA，eNOS and iNOS were found by Topological Analysis. Molecular docking showed that eNOS and iNOS proteins had good affinity with Quercetin，Kaempferol and Isorhamnetin. IHC experiment showed that compared with sham operation group，the expression level of eNOS in model group was lower （P<0.05）；compared with model group，the expression level of eNOS and iNOS in Yinchenhao Decoction group were increased （P<0.01）. Conclusion: The effect of Yinchenhao Decoction on obstructive jaundice has the characteristics of multi-component，multi-target，and multi-pathway. The regulation of Yinchenhao Decoction on oxidative stress of obstructive jaundice may be related to the change of eNOS and iNOS protein expression.

参考文献/References:

[1] 金龙，邹英华. 梗阻性黄疸经皮肝穿刺胆道引流及支架植入术专家共识（2018）[J]. 中国介入影像与治疗学，2019，16（1）：2
[2] ATALAY E，OZDEMIR M T，TUR B K，et al. The effect of alpha lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice[J]. Bratisl Lek Listy，2019，120（11）：843
[3] UNAL Y，TUNCAL S，KOSMAZ K，et al. The effect of calcium dobesilate on liver damage in experimental obstructive jaundice[J]. J Invest Surg，2019，32（3）：238
[4] 陈秋源，钟小生，谭志健. 梗阻性黄疸的中西医治疗进展[J]. 现代中西医结合杂志，2016，25（2）：221
[5] 张华敏，刘思鸿，高宏杰，等. 复方中药网络药理学方法研究进展[J].中国医院用药评价与分析，2019，19（10）：1270
[6] 李鑫，王向莹，王诗源. 《伤寒杂病论》与《中医内科学》互参探讨黄疸的分型辨治[J]. 山东中医药大学学报，2020，44（1）：19
[7] DOUSTIMOTLAGH A H，KOKHDAN E P，VAKILPOUR H，et al. Protective effect of Nasturtium officinale R. Br and quercetin against cyclophosphamide-induced hepatotoxicity in rats[J]. Mol Biol Rep，2020，47（7）：5001
[8] WU L，ZHANG Q，MO W，et al. Quercetin prevents hepatic fibrosis by inhibiting hepatic stellate cell activation and reducing autophagy via the TGF-β1/Smads and PI3K/Akt pathways[J]. Sci Rep，2017，7（1）：9289
[9] XU T，HUANG S，HUANG Q，et al. Kaempferol attenuates liver fibrosis by inhibiting activin receptor-like kinase 5[J]. J Cell Mol Med，2019，23（9）： 6403
[10] DU Y C，LAI L，ZHANG H，et al. Kaempferol from Penthorum chinense Pursh suppresses HMGB1/TLR4/NF-κB signaling and NLRP3 inflammasome activation in acetaminophen-induced hepatotoxicity[J].Food Funct，2020，11（9）：7925
[11] YIN Y，LIU X，LIU J，et al. Beta-sitosterol and its derivatives repress lipopolysaccharide/d-galactosamine-induced acute hepatic injury by inhibiting the oxidation and inflammation in mice[J]. Bioorg Med Chem Lett，2018，28（9）：1525
[12] 华圆，冯健，李范珠.茵陈蒿汤利胆退黄物质基础的研究进展[J].中华中医药学刊，2011，29（7）：1520
[13] REYES-GORDILLO K，SHAH R，Arellanes-Robledo J，et al. Akt1 and Akt2 isoforms play distinct roles in regulating the development of inflammation and fibrosis associated with alcoholic liver disease[J]. Cells，2019，8（11）：1337
[14] 曾怡，潘青波，沈妍希，等. 中和白细胞介素-6减轻小鼠急性肝损伤[J]. 中华肝脏病杂志，2020，28（6）：509
[15] TEMEL Y，KUCUKLER S，YILDIRIM S，et al. Protective effect of chrysin on cyclophosphamide-induced hepatotoxicity and nephrotoxicity via the inhibition of oxidative stress，inflammation，and apoptosis[J]. Naunyn Schmiedebergs Arch Pharmacol，2020，393（3）：325
[16] JIN X，AIMAITI Y，CHEN Z，et al. Hepatic stellate cells promote angiogenesis via the TGF-β1-Jagged1/VEGFA axis[J]. Exp Cell Res，2018，373（1/2）：34
[17] WANG A L，JIANG H，LIU Y，et al. Rhein induces liver cancer cells apoptosis via activating ROS-dependent JNK/Jun/caspase-3 signaling pathway[J]. J Cancer，2020，11（2）：500
[18] CHENG M H，REN H Z ，WANG J，et al. Targeting PI3K/Akt/Nrf2 pathway by glabridin alleviates acetaminophen-induced hepatic injury in rats[J]. Arab J Chem，2020，14（prepublish）
[19] 陈晔，刘丹，李灵芝，等. 蕨麻多酚对糖氧剥夺损伤血管内皮细胞一氧化氮、一氧化氮合酶、内皮素及缺氧诱导因子的影响[J]. 中国药师，2019，22（3）：389
[20] 张西波，段启龙，李忠廉. 一氧化氮在阻塞性黄疸肝细胞损伤中的作用研究进展[J]. 山东医药，2014，54（40）：102

相似文献/References:

[1]韩树旺,尚海涛,张德林,等.茵陈五苓散对湿重于热型阻塞性黄疸胆道术后T细胞亚群的影响[J].天津医科大学学报,2024,30(01):1.[doi:10.20135/j.issn.1006-8147.2024.01.0001]
　HAN Shuwang,SHANG Haitao,ZHANG Delin,et al.Effect of Yinchen Wuling Powder on T cell subsets in patients with obstructive jaundice after biliary tract surgery[J].Journal of Tianjin Medical University,2024,30(06):1.[doi:10.20135/j.issn.1006-8147.2024.01.0001]
[2]缪道玉,于方海 综述,张西波 审校.调节性细胞死亡方式参与阻塞性黄疸引起肝细胞损伤的机制研究[J].天津医科大学学报,2024,30(04):377.[doi:10.20135/j.issn.1006-8147.2024.04.0377]
[3]孙一萌,刘浩,刘军舰,等.茵陈蒿汤对阻塞性黄疸大鼠肝细胞ATF6/GRP78/CHOP凋亡信号通路的影响[J].天津医科大学学报,2023,29(03):252.
　SUN Yi-meng,LIU Hao,LIU Jun-jian,et al.Influence of Yinchenhao decoction on the expression of ATF6/GRP78/CHOP apoptotic signaling pathway in hepatocyte of rats with obstructive jaundice[J].Journal of Tianjin Medical University,2023,29(06):252.

备注/Memo

备注/Memo:

基金项目 国家自然科学基金面上项目（81273952）；天津市中医药重点领域科技项目(2019003) 作者简介 陈帅（1993-），男，医师，硕士在读，研究方向：中西医结合治疗肝胆胰外科疾病；通信作者：李忠廉，E-mail：nkyylzl@163.com。

常用功能

更新日期/Last Update: 2021-11-15