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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

27卷

期数:

2021年06期

页码:

561-563,568

栏目:

心血管疾病专题

出版日期:

2021-11-15

文章信息/Info

Title:

Study on specific target genes of heart failure after acute myocardial infarction

文章编号:

1006-8147（2021）06-0561-03

作者:

李树杰¹; 2; 宋昱²

（1.天津医科大学研究生院，天津300070；2.天津泰达国际心血管病医院ICU，天津300457）

Author(s):

LI Shu-jie¹; 2; SONG Yu²

（1.Graduate School，Tianjin Medical University，Tianjin 300070，China；2. ICU of Tianjin TEDA International Cardiovascular Hospital，Tianjin，300457，China）

关键词:

急性心肌梗死; 心力衰竭; 差异性基因比较

Keywords:

acute myocardial infarction; heart failure;  differentially gene expression

分类号:

R543.3

DOI:

-

文献标志码:

A

摘要:

目的：生物信息库系统性比较急性心肌梗死后心力衰竭患者相对于非心力衰竭患者的差异性基因(mRNA和miRNAs)，临床进行重点差异基因验证。方法： 基础研究中，基于GEO数据库(编号GSE59867)中17例急性心肌梗死患者在发病后6个月随访期间内心力衰竭发展的详细情况，分析心力衰竭患者相对于非心力衰竭患者基因表达的差异，构建心力衰竭患者基因表达网络。临床研究中，回顾性收集天津泰达国际心血管病医院2018年1月—2020年12月经病理确诊的急性心肌梗死患者61例，根据心力衰竭与否，分为心力衰竭组和非心力衰竭组。利用ELISA方法比较验证特征基因的表达差异。结果：基础研究发现，与急性心肌梗死后非心力衰竭患者比较，心力衰竭患者共显示703个差异表达基因（mRNAs），包括567个上调基因和136个下调基因。其中SPI1、MCUR1、ZBTB7A、IRF8和PPARG表现出最显著上调。与此同时，心力衰竭患者表现出12个差异miRNAs，其中上调的miRNAs 5个，下调的miRNAs 7个。miR-133、miR-124-3和miR-9-1显著下调，差异均具有统计学意义（P<0.05）。临床研究发现，SPI1、MCUR1、ZBTB7A、IRF8和PPARG蛋白表达浓度在急性心肌梗死后心力衰竭组中有了显著的升高（SPI1：t=4.36，P<0.01；MCUR1：t=4.98，P<0.01；ZBTB7A：t=3.77, P<0.01；IRF8：t=3.12，P<0.01；PPARG：t=5.13，P<0.01)。结论：相对于心肌梗死后非心力衰竭患者，心力衰竭患者表现出若干差异基因（SPI1、MCUR1、ZBTB7A、IRF8和PPARG），可以作为潜在临床标志物进行研究。

Abstract:

Objective： To systematically compare the differential genes（mRNA and miRNAs） between patients with heart failure after acute myocardial infarction and non-heart failure patients in the bioinformation database，and to verify the key differential genes in the clinic. Methods： In the basic study，based on the GEO database（GSE59867），17 patients with acute myocardial infarction were followed up for 6 months after the onset of heart failure.The difference of gene expression between heart failure patients and non-heart failure patients were analyzed，and the gene expression network of heart failure patients was constructed. In the clinical study，61 patients with acute myocardial infarction diagnosed by pathology from January 2018 to December 2020 at TEDA international cardiovascular hospital were retrospectively divided into heart failure group and non-heart failure group according to heart failure. The difference of characteristic gene expression was verified by ELISA method. Results： The basic research showed that 703 differentially expressed genes（mRNAs），including 567 up-regulated genes and 136 down-regulated genes，were displayed in patients with heart failure，compared with patients without heart failure after acute myocardial infarction. SPI1，MCUR1，ZBTB7A，IRF8 and PPARG demonstrated the most significant increase. Meanwhile，patients with heart failure showed 12 different miRNAs，including 5 up-regulated miRNAs and 7 down-regulated miRNAs. MiR-133，miR-124-3 and miR-9-1 decreased significantly，the differences were statistically significant（P<0.05）.Clinical studies showed that the expression levels of SPI1，MCUR1，ZBTB7A，IRF8 and PPARG were significantly increased in patients with heart failure （SPI1： t=4.36，P<0.01；MCUR1：t=4.98，P<0.01；ZBTB7A：t=3.77，P<0.01；IRF8：t=3.12，P<0.01；PPARG：t=5.13，P<0.01）.Conclusion： Some differentially expressed genes（SPI1，MCUR1，ZBTB7A，IRF8 and PPARG） may be used as potential clinical markers in patients with heart failure，compared with patients without heart failure after myocardial infarction.

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备注/Memo

备注/Memo:

基金项目 天津市卫生局科技基金重点项目(2013KZ006) 作者简介 李树杰（1989-），男，医师，硕士在读，研究方向：心血管相关疾病; 通信作者：宋昱，E-mail：773842817@qq.com。

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更新日期/Last Update: 2021-11-15