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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

26卷

期数:

2020年02期

页码:

118-121,157

栏目:

基础医学

出版日期:

2020-04-30

文章信息/Info

Title:

Effect of renal sympathetic denervation on hypothalamus AngⅡ and AT1R of spontaneously hypertensive rats

文章编号:

1006-8147(2020)02-0118-05

作者:

安玉楠¹; 马艺杰¹; 王丽¹; 2; 李竹青¹; 卢成志¹; 2

（1 天津医科大学研究生院，天津300070 ；2 天津市第一中心医院心内科，天津 300192 ）

Author(s):

AN Yu-nan¹;  MA Yi-jie¹;  WANG-Li¹; 2;  LI Zhu-qing¹;  LU Cheng-zhi¹; 2

（1.Graduate School,Tianjin Medical University,Tianjin 300070,China; 2.Department of Cardiology, Tianjin First Central Hospital,Tianjin 300192,China）

关键词:

高血压; 去肾交感神经术; 神经元特异性烯醇化酶

Keywords:

hypertension; renal sympathetic denervation; neuronspecific enolase

分类号:

R544.1

DOI:

-

文献标志码:

A

摘要:

目的：为了进一步明确去肾交感神经术（RDN）作用于高血压大鼠的降压机制，探究RDN对下丘脑AngII、AT1R含量表达的影响。方法：36只自发性高血压大鼠（SHR）及12只同周龄Wistar-Kyoto 大鼠（WKY），采用随机数字表的方法将SHR分为对照组（DC组，n=12）、RDN手术组（术后1周为DO1组，n=6；术后6周为DO6，n=6）、假手术组（术后1周为DS1组，n=6；术后6周为DS6， n=6）；WKY组为对照组（NC组，n=12）。分别于基线时、术后1、6周测量大鼠血压，检测血浆S100B蛋白（S100B）、神经元特异性烯醇化酶（NSE）水平，肾脏去甲肾上腺素（NE）及下丘脑血管紧张素II（AngII）、血管紧张素II的1型受体（AT1R）mRNA含量的变化。结果：与DC组比较，DO1组大鼠的收缩压下降39.8 mmHg,舒张压下降30.8 mmHg、肾脏NE、下丘脑AngII、下丘脑AT1RmRNA含量分别降低19.5 pg/mL、6.32 pg/mL 、0.29 pg/mL（均P<0.05），且较DS1组大鼠的收缩压下降38.8 mmHg,舒张压下降29.1 mmHg、肾脏NE、下丘脑AngII、下丘脑AT1RmRNA含量分别降低39.4 pg/mL、10.2 pg/mL 、0.43 pg/mL（均P<0.05）；DO1组大鼠较DC组大鼠血浆NSE及S100B明显升高，分别升高4.6 pg/mL、0.46 pg/mL （均P<0.05），DS1组与DC组相比，NSE、S100B变化不明显（均P＞0.05）。结论：RDN可能是通过抑制中枢血管紧张素的释放及其受体合成，从而降低高血压大鼠的血压，NSE、S100B可能成为评估去肾交感神经术操作成功与否的标志物。

Abstract:

Objective: To explore the roles of renal sympathetic denervation (RDN) in the action of antihypertension，the change of hypothalamus AngII and AT1R mRNA expression in spontaneously hypertensive rats was conducted before and after renal sympathetic nerve surgery. Methods：Thirty-six spontaneously hypertensive rats (SHR) and 12 Wistar-Kyoto rats (WKY) were randomly assigned to DC group(n=12), RDN group [divided into DO1(n=6)and DO6(n=6) subgroup], sham-RDN group [divided into DS1(n=6)and DS6(n=6)subgroup], and control group(NC grounp, n=12) respectively. Blood pressure, the level of plasma S100B protein, neuronspecific enolase(NSE), norepinephrine (NE), angiotensin (Ang II) level, and angiotensin II type 1 (AT1R) mRNA of hypothalamus were detected in 1 and 6 week after RDN. Results: Compared with the DC group, the systolic blood pressure of the DO1 group decreased by 39.8 mmHg, the diastolic blood pressure decreased by 30.8 mmHg, the level of renal NE, hypothalamic Ang II and hypothalamic AT1R mRNA decreased by 19.5 pg/mL, 6.32 pg/mL, and 0.29 pg/mL, respectively(all P<0.05). The systolic blood pressure decreased by 38.8 mmHg and the diastolic blood pressure decreased by 29.1 mmHg compared with the DS1 group, the expression of NE, Ang II and AT1R mRNA decreased by 39.4 pg/mL, 10.2 pg/mL and 0.43 pg/mL, respectively(all P<0.05). The level of plasma NSE and S100B in the DO1 group were significantly higher than those in the DC group, which increased by 4.6 pg/mL and 0.46 pg/mL, respectively (both P<0.05). The chang of NSE and S100B in the DS1 group had no significant change compared with the DC group(both P>0.05). Conclusion: RDN may reduce the blood pressure of hypertensive rats by inhibiting the release of central angiotensin and its receptor synthesis. NSE and S100B may be used as markers to evaluate the success of renal sympathetic denervation.

参考文献/References:

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备注/Memo

备注/Memo:

作者简介 安玉楠（1991-），女，硕士在读，研究方向：冠心病；
通信作者:卢成志，E-mail：30817025@nankai.edu.cn。

常用功能

更新日期/Last Update: 2020-06-02