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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

25卷

期数:

2019年04期

页码:

329-333

栏目:

临床医学

出版日期:

2019-07-20

文章信息/Info

Title:

Expressions of C1QL1 protein inprimaryhepatocellular carcinoma and its clinical significance

文章编号:

1006-8147(2019)04-0329-05

作者:

胡 源¹; 许戈良²; 荚卫东²; 潘婷婷²; 余继海²; 黄 玫³; 周杭城⁴

（1.天津医科大学研究生院，天津300070；2.中国科学技术大学附属第一医院（安徽省立医院）肝脏外科，合肥 230001；3.肝胆胰安徽省重点实验室，合肥230001；4.中国科学技术大学附属第一医院（安徽省立医院）病理科，合肥230001）

Author(s):

HU Yuan¹;  XU Ge-liang²;  JIA Wei-dong²;  PAN Ting-ting²;  YU Ji-hai²;  HUANG Mei³; ZHOU Hang-cheng⁴

(1.Graduate School, Tianjin Medical University, Tianjin 300070, China; 2.Department of Hepatic Surgery, The First Affiliated Hospital, University of Science and Technology of China (Anhui Provincial Hospital),Hefei 230001, China; 3.Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, Hefei 230001, China; 4. Department of Pathology, The First Affiliated Hospital, University of Science and Technology of China (Anhui Provincial Hospital),Hefei 230001, China)

关键词:

肝细胞癌;  C1QL1; 免疫组织化学; Western blot; qRT-PCR

Keywords:

hepatocellular carcinoma; C1QL1; immunohistochemistry; western blot; real-time fluorescent qunatiative PCR

分类号:

R735.7

DOI:

-

文献标志码:

A

摘要:

目的：探讨C1QL1（C1q-like-1）蛋白在原发性肝细胞癌（HCC）中的表达及其临床意义。方法:采用免疫组织化学技术检测80例HCC组织中C1QL1蛋白的表达情况及其临床意义，用qRT-PCR及 Western blot 方法检测20对冷冻HCC组织及癌旁组织中C1QL1蛋白的表达。结果：80例石蜡组织标本的免疫组化结果显示，55例（68.8%）HCC组织中C1QL1蛋白呈高表达；而癌旁组织中8例(10%)C1QL1蛋白呈高表达，两组间表达差异具有统计学意义（P <0.001）；C1QL1在HCC中的表达与有无乙肝病史（P=0.021）、肿瘤的大小（P=0.003）、血管侵犯（P=0.003）、TNM分期（P=0.002）及Edmondson分级（P=0.002）相关，而与年龄（P=0.758）、性别(P=0.672)、甲胎蛋白（AFP）(P=0.191)、肿瘤数目(P=0.877)、Child-Pugh分级（P=0.866）、肿瘤包膜完整程度(P=0.238)和肝硬化程度(P=0.316)均无关。qRT-PCR及Western blot 均显示HCC组织中C1QL1的表达量显著高于对应的癌旁组织，组间差异具有统计学意义( P<0. 01，P<0. 001) 。结论:在HCC组织中，C1QL1蛋白呈高表达，而C1QL1的高表达可能与HCC的发生发展及恶性程度密切相关。

Abstract:

Objective: To investigate the expression and clinical significance of C1QL1 in hepatocellular carcinoma (HCC). Methods:The expression level of C1QL1 in paraffin-embedded HCC tissues and adjacent tissues of HCC patients was measured by 80 cases of hepatectomy and postoperative pathology, and its relationship with clinicopathological was studied. Theq RT-PCR and Western blot were used to detect the expression of C1QL1 in 20 pairs of freshly frozen HCC and adjacent tissues. Results: Immunohistochemical results of paraffin tissue showed: the high expression rate of C1QL1 in HCC tissues was 68.8%(55/80), the high expression rate in adjacent tissues was 10%（8/80）, and the expression difference between the two groups was statistically significant(P< 0.001). The expression of C1QL1 in HCC was correlated with the history of hepatitis B（P=0.021）, tumor size（P=0.003）, vascular invasion（P=0.003）, TNM staging(P=0.002) and Edmondson classification (P=0.002), but was not correlated with age（P=0.758）, sex(P=0.672), alpha fetoprotein(AFP)(P=0.191), the number of tumor(P=0.877), child-pugh classification(P=0.866), tumor envelope integrity(P=0.238) and degree of liver cirrhosis(P=0.316). BothqRT-PCR and Western blot showed that the expression of C1QL1 in HCC tissues was significantly higher than that in the corresponding paracancerous tissues, and the difference between the groups was statistically significant( P <0. 01，P <0. 001) . Conclusion:The expression of C1QL1 protein in HCC tissues is high.The high expression rate of C1QL1 may be closely related to the occurrence, progression and grade malignancy of hepatocellular carcinoma.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81501354）
作者简介 胡源（1993-），男，硕士在读；研究方向：肝肿瘤专题研究；通信作者:许戈良， E-mail:ahsyxgl@163.com。

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更新日期/Last Update: 2019-08-28