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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

22卷

期数:

2016年05期

页码:

406-408

栏目:

基础医学

出版日期:

2016-09-20

文章信息/Info

Title:

Effect of vitamin D supplementation on the changes of serum inflammatory cytokines in severely scalded mice

文章编号:

1006-8147（2016）05-0406-03

作者:

韩夏¹; 张明谏²; 李小兵² ; 刘红杰² ; 王玉亮³; 刘子健²

(1.天津医科大学一中心临床学院整形与烧伤外科,天津300192; 2.天津市第一中心医院整形与烧伤外科,天津300192;3.卫生部危重病急救医学重点实验室,天津 300384)

Author(s):

HAN Xia¹; ZHANG Ming-jian²; LI Xiao-bing²; LIU Hong-jie²; WANG Yu-liang³; LIU Zi-jian²

（1. Department of Burns and Plastic Surgery ，The First Central Clinical College ， Tianjin Medical University，Tianjin 300192，China；2. Department of Burns and Plastic Surgery ，The First Central Hospital of Tianjin，Tianjin 300192，China；3. The Key Laboratory of Critical Care of Medical Ministry of Health，Tianjin 300384，China）

关键词:

维生素D; 烫伤; 炎性因子; 小鼠

Keywords:

vitamin D; scald; inflammatory cytokines; mice

分类号:

R644

DOI:

-

文献标志码:

A

摘要:

目的：观察补充维生素D对严重烫伤小鼠血清炎性因子浓度变化的影响。方法：(1)随机将88只小鼠分为实验组和对照组各40只，空白对照组8只。(2)实验组小鼠烫伤后即刻给予1,25(OH)₂D₃ 4 μg/kg+0.6 mL花生油灌胃，每日1次，至处死前；对照组烫伤小鼠0.6 mL花生油灌胃，每日1次，至处死前。(3)于伤后6、12、24、48、96 h各组分别随机选择8只小鼠，用ELISA法检测血清TNF-α、IL-1β、IL-4、IL-10含量。(4)于伤后96 h取两组小鼠肠系膜、肝和脾淋巴结组织分别制成匀浆，做48 h细菌培养计算菌落形成单位数量。结果：(1)烫伤后12、24、48、96 h，实验组和对照组小鼠血清TNF-α、IL-1β、IL-4、IL-10浓度分别高于空白对照组水平（P<0.05）。(2)烫伤后24、48、96 h，实验组小鼠血清TNF-α和IL-1β浓度分别低于对照组水平（P<0.05）；烫伤后12、24、48、96 h，实验组小鼠血清IL-4和IL-10浓度分别高于对照组水平（P<0.05）。(3)伤后96 h实验组小鼠肠系膜、肝和脾淋巴结组织菌落形成单位量分别低于对照组(P<0.05)。结论：严重烫伤小鼠伤后补充维生素D能够降低TNF-α和IL-1β浓度、提高IL-4和IL-10浓度，并减少肠系膜、肝和脾淋巴结组织菌落形成单位数量

Abstract:

Objective ：To explore the effect of vitamin D supplementation on the changes of serum inflammatory cytokines in severely scalded mice. Methods：(1) Eighty-eight mice were assigned into 3 groups randomly: treatment group(40), control group(40) and blank group(8). (2)Mice in treatment group were immediately fed 0.6 mL peanut oil with1,25(OH)₂D₃(4 μg/kg) once a day post-burn, and mice in control group received only 0.6mL peanut oil without 1,25(OH)₂D₃. (3)The levels of serum TNF-α, IL-1β, IL-4 and IL-10 were determined respectively by ELISA at the time of 6, 12, 24, 48 h and 96h post-burn. (4)The colony-forming units of liver, spleen and mesenteric lymph nodes of mice in two groups were calculated respectively at 96 h post-burn. Results：(1)The serum TNF-α, IL-1β, IL-4 and IL-10 levels in treatment and control group were significantly higher than those in blank group at the time of 12, 24, 48 h and 96h post-burn(P＜0.05). (2)The serum TNF-α and IL-1β levels in treatment group were lower than those in control group at the time of 24, 48 and 96 h post-burn(P＜0.05), and the serum IL-4 and IL-10 levels in mice in treatment group were higher than those in control group at the time of 12, 24, 48 h and 96 h post-burn(P＜0.05). (3)The numbers of colony-forming units of liver, spleen and mesenteric lymph nodes of mice in treatment group were lower than those in control group at 96 h post-burn(P＜0.05). Conclusion：Supplementing vitamin D could reduce the levels of serum TNF-α and IL-1β, and increase the level of serum IL-4 and IL-10 in severely scalded mice. And it could reduce the numbers of colony-forming units of liver, spleen and mesenteric lymph nodes of mice post-burn.

参考文献/References:

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备注/Memo

备注/Memo:

作者简介 韩夏（1990-），男，硕士在读，研究方向：外科学（烧伤）；

通信作者：张明谏，E-mail:zhangmingjian@hotmail.com。

常用功能

更新日期/Last Update: 2016-09-19